C57BL/6JCya-Map3k7em1flox/Cya
Common Name:
Map3k7-flox
Product ID:
S-CKO-09570
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Map3k7-flox
Strain ID
CKOCMP-26409-Map3k7-B6J-VA
Gene Name
Product ID
S-CKO-09570
Gene Alias
B430101B05; Tak1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Map3k7em1flox/Cya mice (Catalog S-CKO-09570) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037607
NCBI RefSeq
NM_009316
Target Region
Exon 4~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Map3k7, also known as TGF-β -activated kinase 1 (TAK1), is a member of the MAPK kinase kinase (MAPKKK) family. It plays a crucial role in regulating a wide range of physiological and pathological processes, participating in pathways such as NF -κB and MAPKs activation. It functions through assembling with TAK1 -binding proteins (TAB1, TAB2, and TAB3) and can be activated by stimuli like TNFα, IL -1β, and toll -like receptor ligands [3].
In prostate cancer, Map3k7 knockout increased AR protein levels and activity in the mouse prostate, and MAP3K7 and AR protein levels were inversely correlated in patient specimens. This indicates that the inflammation -activated MAP3K7-IKKβ axis has a tumor-suppressive function in degrading AR protein [1].
In hypertensive nephropathy, TRIM31 knockout mice showed aggravated hypertension-induced renal dysfunction, fibrosis, and inflammation, as TRIM31 promotes the proteasomal degradation of MAP3K7, negatively regulating TGF -β1-mediated Smad and MAPK/NF -κB signaling pathways [2].
In mouse embryonic stem cells, inhibiting Map3k7 with a specific inhibitor dramatically decreased endoderm differentiation, suggesting that Map3k7 governs the formation or proliferation of cardiogenic endoderm [4].
In conclusion, Map3k7 is essential in multiple biological processes and disease conditions. Gene knockout models in mice have revealed its significant role in prostate cancer, hypertensive nephropathy, and endoderm differentiation. Understanding Map3k7 functions can provide insights into disease mechanisms and potential therapeutic strategies for related diseases.
References:
1. Huang, Zhenlin, Tang, Bo, Yang, Yinhui, Jia, Zhankui, Huang, Haojie. 2021. MAP3K7-IKK Inflammatory Signaling Modulates AR Protein Degradation and Prostate Cancer Progression. In Cancer research, 81, 4471-4484. doi:10.1158/0008-5472.CAN-20-4194. https://pubmed.ncbi.nlm.nih.gov/34158377/
2. Zhang, Jie, Cao, Lei, Wang, Xiaohong, Zhang, Meng, Zhang, Yun. 2021. The E3 ubiquitin ligase TRIM31 plays a critical role in hypertensive nephropathy by promoting proteasomal degradation of MAP3K7 in the TGF-β1 signaling pathway. In Cell death and differentiation, 29, 556-567. doi:10.1038/s41418-021-00874-0. https://pubmed.ncbi.nlm.nih.gov/34584221/
3. Xu, Yan-Ran, Lei, Cao-Qi. 2021. TAK1-TABs Complex: A Central Signalosome in Inflammatory Responses. In Frontiers in immunology, 11, 608976. doi:10.3389/fimmu.2020.608976. https://pubmed.ncbi.nlm.nih.gov/33469458/
4. Hunter, Andrew, Dai, Yunkai, Brown, Kemar J, Muise-Helmericks, Robin C, Foley, Ann C. 2019. TAK1/Map3k7 enhances differentiation of cardiogenic endoderm from mouse embryonic stem cells. In Journal of molecular and cellular cardiology, 137, 132-142. doi:10.1016/j.yjmcc.2019.10.002. https://pubmed.ncbi.nlm.nih.gov/31668971/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen