C57BL/6NCya-Ahcyem1flox/Cya
Common Name:
Ahcy-flox
Product ID:
S-CKO-09788
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ahcy-flox
Strain ID
CKOCMP-269378-Ahcy-B6N-VA
Gene Name
Product ID
S-CKO-09788
Gene Alias
CuBP; SAHH
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ahcyem1flox/Cya mice (Catalog S-CKO-09788) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054607
NCBI RefSeq
NM_016661
Target Region
Exon 4~8
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Ahcy, also known as adenosylhomocysteinase, is a highly conserved enzyme. It catalyzes the reversible breakdown of S-adenosylhomocysteine (SAH), a by-product and potent inhibitor of methyltransferases activity. This places Ahcy in the methionine cycle, which is crucial for cellular methylation levels. By removing excess SAH, Ahcy facilitates local transmethylation reactions, playing an essential role in DNA, RNA, and histone methylation during processes like replication and transcription [1].
In mouse and human adipocyte progenitor cells, inhibition of Ahcy using adenosine dialdehyde (AdOx) or siRNA-mediated knockdown reduces cell proliferation and number, as well as adipocyte differentiation and the expression of adipogenic markers. This indicates that Ahcy is necessary for the maintenance of these processes [2]. In colorectal cancer, loss of Apc in mouse intestine drives the expression of Ahcy, and targeting Ahcy function impairs the growth of APC-deficient organoids in vitro and prevents the characteristic hyperproliferative phenotype in vivo. Pharmacological inhibition of Ahcy also reduces intestinal tumour burden in ApcMin/+ mice, suggesting its potential as a metabolic drug target in CRC [3].
In conclusion, Ahcy is vital for maintaining normal cellular methylation levels and is essential for processes such as cell proliferation and differentiation. Mouse models, especially those with genetic alterations relevant to diseases like colorectal cancer, have revealed its potential as a therapeutic target in certain disease conditions, highlighting the importance of Ahcy in both normal biological functions and disease-related processes.
References:
1. Vizán, Pedro, Di Croce, Luciano, Aranda, Sergi. 2021. Functional and Pathological Roles of AHCY. In Frontiers in cell and developmental biology, 9, 654344. doi:10.3389/fcell.2021.654344. https://pubmed.ncbi.nlm.nih.gov/33869213/
2. Boczki, Paula, Colombo, Marco, Weiner, Juliane, Körner, Antje, Landgraf, Kathrin. 2023. Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells. In Adipocyte, 13, 2290218. doi:10.1080/21623945.2023.2290218. https://pubmed.ncbi.nlm.nih.gov/38064408/
3. Vande Voorde, Johan, Steven, Rory T, Najumudeen, Arafath K, Bunch, Josephine, Sansom, Owen J. 2023. Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target. In Nature metabolism, 5, 1303-1318. doi:10.1038/s42255-023-00857-0. https://pubmed.ncbi.nlm.nih.gov/37580540/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen