C57BL/6JCya-Txlnbem1flox/Cya
Common Name:
Txlnb-flox
Product ID:
S-CKO-10838
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Txlnb-flox
Strain ID
CKOCMP-378431-Txlnb-B6J-VA
Gene Name
Product ID
S-CKO-10838
Gene Alias
2310001N14Rik; Mdp77
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Txlnbem1flox/Cya mice (Catalog S-CKO-10838) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037964
NCBI RefSeq
NM_138628
Target Region
Exon 3
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Txlnb, also known as β -taxilin, is a cardiomyocyte-enriched protein belonging to a family of centrosome adapter proteins involved in protein quality control. It is associated with the ubiquitin-proteasome system (UPS) and centrosome-related processes, playing a potential role in maintaining proteostasis. Genetic models, such as gene knockout mouse models, are valuable for studying its functions [1,2].
In Txlnb-knockout (KO) mouse models, there is proteasomal insufficiency and altered cardiac growth. Ubiquitinated protein accumulates, 26Sβ5 proteasome activity decreases, and cardiac mass lowers with age. In mouse models of cardiac proteotoxicity (CryAB-R120G mice) and pressure overload (transverse aortic constriction, TAC), loss of Txlnb worsens heart phenotypes, including lower ejection fractions, increased heart mass, and elevated ubiquitinated protein accumulation. Txlnb-KO also leads to up-regulation of Txlna and several centrosomal proteins, suggesting altered centrosomal dynamics [1,2].
In conclusion, Txlnb is a key regulator of cardiac proteostasis, revealed through KO mouse models. Its role in maintaining proteostasis in cardiomyocytes is crucial, and understanding its functions provides insights into cardiac-related disease mechanisms, such as heart failure and cardiac proteotoxicity.
References:
1. McLendon, Jared M, Zhang, Xiaoming, Stein, Colleen S, Bodine, Sue C, Boudreau, Ryan L. 2024. A Specialized Centrosome-Proteasome Axis Mediates Proteostasis and Influences Cardiac Stress through Txlnb. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.02.12.580020. https://pubmed.ncbi.nlm.nih.gov/38405715/
2. McLendon, Jared M, Zhang, Xiaoming, Stein, Colleen S, Bodine, Sue C, Boudreau, Ryan L. 2025. Gain and loss of the centrosomal protein taxilin-beta influences cardiac proteostasis and stress. In Journal of molecular and cellular cardiology, 201, 56-69. doi:10.1016/j.yjmcc.2025.02.008. https://pubmed.ncbi.nlm.nih.gov/40010430/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen