C57BL/6JCya-Fmn2em1flox/Cya
Common Name:
Fmn2-flox
Product ID:
S-CKO-11755
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fmn2-flox
Strain ID
CKOCMP-54418-Fmn2-B6J-VA
Gene Name
Product ID
S-CKO-11755
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fmn2em1flox/Cya mice (Catalog S-CKO-11755) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030039
NCBI RefSeq
NM_019445
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Fmn2, a member of the formin family, is crucial for various cellular processes. It plays a significant role in brain morphogenesis by regulating growth cone motility, which is essential for axonal outgrowth and accurate synaptic targeting. It also participates in axonal collateral branching, a key process in the development of functional neural circuits [1,2].
In growth cone motility, Fmn2 functions as a clutch molecule, mediating the coupling of the actin cytoskeleton to the growth substrate via point contact adhesion complexes, thereby generating traction stresses necessary for growth cone translocation [1]. In axonal collateral branching, Fmn2 localizes to axonal F-actin patches, regulating their lifetime and size, and protecting them from disassembly by the actin-depolymerizing factor ADF, which is important for the initiation of axonal protrusions that form collateral branches [2].
In conclusion, Fmn2 is a key regulator in neuronal development, especially in growth cone motility and axonal branching. Its malfunction may be associated with neurodevelopmental and neurodegenerative disorders, as well as other diseases such as premature ovarian insufficiency, small cell lung cancer, and colorectal cancer [1,2,3,4,5]. Studies on Fmn2 contribute to a better understanding of the underlying mechanisms of these diseases and may offer potential therapeutic targets.
References:
1. Ghate, Ketakee, Mutalik, Sampada P, Sthanam, Lakshmi Kavitha, Sen, Shamik, Ghose, Aurnab. 2020. Fmn2 Regulates Growth Cone Motility by Mediating a Molecular Clutch to Generate Traction Forces. In Neuroscience, 448, 160-171. doi:10.1016/j.neuroscience.2020.09.046. https://pubmed.ncbi.nlm.nih.gov/33002558/
2. Kundu, Tanushree, Siva Das, Sooraj, Sewatkar, Lisas K, Nagar, Dhriti, Ghose, Aurnab. 2022. Antagonistic Activities of Fmn2 and ADF Regulate Axonal F-Actin Patch Dynamics and the Initiation of Collateral Branching. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 42, 7355-7369. doi:10.1523/JNEUROSCI.3107-20.2022. https://pubmed.ncbi.nlm.nih.gov/36481742/
3. Li, Jie, Peng, Tianliu, Wang, Le, Xiao, Hongmei, Shi, Xiaobo. 2022. Heterozygous FMN2 missense variant found in a family case of premature ovarian insufficiency. In Journal of ovarian research, 15, 31. doi:10.1186/s13048-022-00960-y. https://pubmed.ncbi.nlm.nih.gov/35227295/
4. Yu, Qiyao, Zhang, Yi, Tian, Yanming, Ju, Yingchao, Gao, Chao. 2023. Exosomal Circ_FMN2 Derived from the Serum of Colorectal Cancer Patients Promotes Cancer Progression by miR-338-3p/MSI1 Axis. In Applied biochemistry and biotechnology, 195, 7322-7337. doi:10.1007/s12010-023-04456-3. https://pubmed.ncbi.nlm.nih.gov/36995659/
5. George, Julie, Maas, Lukas, Abedpour, Nima, Peifer, Martin, Thomas, Roman K. 2024. Evolutionary trajectories of small cell lung cancer under therapy. In Nature, 627, 880-889. doi:10.1038/s41586-024-07177-7. https://pubmed.ncbi.nlm.nih.gov/38480884/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen