C57BL/6JCya-Hpgdsem1flox/Cya
Common Name:
Hpgds-flox
Product ID:
S-CKO-11773
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hpgds-flox
Strain ID
CKOCMP-54486-Hpgds-B6J-VA
Gene Name
Product ID
S-CKO-11773
Gene Alias
H-PGDS; Ptgds2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hpgdsem1flox/Cya mice (Catalog S-CKO-11773) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031982
NCBI RefSeq
NM_019455
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Hpgds, short for hematopoietic prostaglandin D synthase, is responsible for the production of prostaglandin D2 (PGD2), an inflammatory mediator. It is involved in the arachidonic acid metabolism pathway, which is of great biological importance as lipid metabolism is crucial for various physiological processes [1,2,3,4]. Genetic models, such as gene knockout (KO) mouse models, can be valuable for studying Hpgds functions.
Hpgds deficiency in type 2 diabetic mice delays normal wound healing, while overexpressing Hpgds in adipose-derived mesenchymal stem cells accelerates diabetic wound healing by reducing neutrophil and CD8T cell recruitment, promoting M2 macrophage polarization and increasing growth factor production [1]. In A549 cell lines, knockdown of Hpgds promotes lipid synthesis and cell migration, and the knockdown promotes migration response by upregulating the expression of lipid metabolism key enzymes ACSL1 and ACC [2].
In conclusion, Hpgds plays a significant role in wound healing and lipid metabolism-related processes. The findings from KO-like models, such as in diabetic mice and cell lines, contribute to understanding its functions in diabetes-related wound healing and lung adenocarcinoma development, providing potential therapeutic targets for these disease areas.
References:
1. Ouyang, Long, Qiu, Daojing, Fu, Xin, Yan, Li, Xiao, Ran. 2022. Overexpressing HPGDS in adipose-derived mesenchymal stem cells reduces inflammatory state and improves wound healing in type 2 diabetic mice. In Stem cell research & therapy, 13, 395. doi:10.1186/s13287-022-03082-w. https://pubmed.ncbi.nlm.nih.gov/35922870/
2. Shao, Fengling, Mao, Huajie, Luo, Tengling, Xu, Lei, Xie, Yajun. 2022. HPGDS is a novel prognostic marker associated with lipid metabolism and aggressiveness in lung adenocarcinoma. In Frontiers in oncology, 12, 894485. doi:10.3389/fonc.2022.894485. https://pubmed.ncbi.nlm.nih.gov/36324576/
3. Chiba, Yoshihiko, Suto, Wataru, Sakai, Hiroyasu. 2018. Augmented Pla2g4c/Ptgs2/Hpgds axis in bronchial smooth muscle tissues of experimental asthma. In PloS one, 13, e0202623. doi:10.1371/journal.pone.0202623. https://pubmed.ncbi.nlm.nih.gov/30161143/
4. Liu, Yong, Liang, Youcheng, Su, Yongjian, Zheng, Mingbin, Huang, Zunnan. 2023. Exploring the potential mechanisms of Yi-Yi-Fu-Zi-Bai-Jiang-San therapy on the immune-inflamed phenotype of colorectal cancer via combined network pharmacology and bioinformatics analyses. In Computers in biology and medicine, 166, 107432. doi:10.1016/j.compbiomed.2023.107432. https://pubmed.ncbi.nlm.nih.gov/37729701/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen