C57BL/6JCya-Txnipem1flox/Cya
Common Name:
Txnip-flox
Product ID:
S-CKO-12038
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Txnip-flox
Strain ID
CKOCMP-56338-Txnip-B6J-VA
Gene Name
Product ID
S-CKO-12038
Gene Alias
1200008J08Rik; Hyplip1; THIF; Tbp-2; VDUP1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Txnipem1flox/Cya mice (Catalog S-CKO-12038) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000074519
NCBI RefSeq
NM_001009935
Target Region
Exon 2~8
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Txnip, also known as thioredoxin interacting protein or VDUP1 (vitamin D3 upregulated protein-1), is an alpha-arrestin protein crucial for redox homeostasis in the human body. It binds to thioredoxin (TRX) to inhibit TRX function and expression, and is involved in multiple pathways such as those related to glucose and lipid metabolism, cell cycle arrest, and inflammation [3,4].
In non-alcoholic steatohepatitis (NASH), deletion of the Txnip gene in mice enhanced hepatic steatosis, inflammation, and fibrosis, accompanied by impaired autophagy and fatty acid oxidation (FAO) [1]. In atherosclerotic calcification, Txnip-/-mice presented increased calcification and collagen deposition, with an expanded osteochondrogenic cluster derived from VSMCs [2]. In diabetic kidney disease, Txnip knockout suppressed renal fibrosis and mTORC1 activation, while restoring TFEB and autophagy activation in diabetic kidneys [5].
In conclusion, Txnip plays essential roles in maintaining redox balance and regulating various biological processes. Gene knockout mouse models have revealed its significance in diseases like NASH, atherosclerotic calcification, and diabetic kidney disease, providing valuable insights into potential therapeutic strategies targeting Txnip for these conditions.
References:
1. Park, Hee-Seon, Song, Ji-Won, Park, Jin-Ho, Won, Young-Suk, Kwon, Hyo-Jung. 2020. TXNIP/VDUP1 attenuates steatohepatitis via autophagy and fatty acid oxidation. In Autophagy, 17, 2549-2564. doi:10.1080/15548627.2020.1834711. https://pubmed.ncbi.nlm.nih.gov/33190588/
2. Woo, Sang-Ho, Kyung, Dongsoo, Lee, Seung Hyun, Choi, Jae-Hoon, Kim, Dae-Yong. 2022. TXNIP Suppresses the Osteochondrogenic Switch of Vascular Smooth Muscle Cells in Atherosclerosis. In Circulation research, 132, 52-71. doi:10.1161/CIRCRESAHA.122.321538. https://pubmed.ncbi.nlm.nih.gov/36448450/
3. Pan, Min, Zhang, Fengping, Qu, Kai, Liu, Chang, Zhang, Jingyao. 2022. TXNIP: A Double-Edged Sword in Disease and Therapeutic Outlook. In Oxidative medicine and cellular longevity, 2022, 7805115. doi:10.1155/2022/7805115. https://pubmed.ncbi.nlm.nih.gov/35450411/
4. Deng, Jinhai, Pan, Teng, Liu, Zaoqu, Beatson, Richard, Ng, Tony. 2023. The role of TXNIP in cancer: a fine balance between redox, metabolic, and immunological tumor control. In British journal of cancer, 129, 1877-1892. doi:10.1038/s41416-023-02442-4. https://pubmed.ncbi.nlm.nih.gov/37794178/
5. Du, Yunxia, Wu, Ming, Song, Shan, Bian, Yawei, Shi, Yonghong. 2024. TXNIP deficiency attenuates renal fibrosis by modulating mTORC1/TFEB-mediated autophagy in diabetic kidney disease. In Renal failure, 46, 2338933. doi:10.1080/0886022X.2024.2338933. https://pubmed.ncbi.nlm.nih.gov/38616177/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen