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C57BL/6JCya-P3h1em1flox/Cya
Common Name:
P3h1-flox
Product ID:
S-CKO-12074
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
P3h1-flox
Strain ID
CKOCMP-56401-P3h1-B6J-VA
Gene Name
P3h1
Product ID
S-CKO-12074
Gene Alias
2410024C15Rik; Gros1; Lepre1
Background
C57BL/6JCya
NCBI ID
56401
Modification
Conditional knockout
Chromosome
4
Phenotype
MGI:1888921
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-P3h1em1flox/Cya mice (Catalog S-CKO-12074) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030393
NCBI RefSeq
NM_001286148
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Click here to download >>
Overview of Gene Research
P3h1, also known as prolyl 3-hydroxylase 1, is crucial for collagen posttranslational processing. It forms a ternary complex with CRTAP and PPIB, playing a key role in the proper assembly and function of collagen [1,9]. The hydroxylation of proline residues in collagen by P3h1 is an important step in collagen biosynthesis, and this process is related to the normal development and structure of tissues [3,7].

In zebrafish, knock out of p3h1 led to phenotypes similar to osteogenesis imperfecta (OI) patients, including reduced size, body disproportion, altered mineralization, vertebral body fusions, deformities, and fractures. Intracellularly, collagen type I was overmodified and partially retained, causing enlarged ER cisternae, and in the extracellular matrix, abnormal collagen type I assembled in disorganized fibers [8]. In humans, mutations in P3H1 are associated with autosomal recessive OI type VIII, which can cause severe skeletal fragility, multiple fractures, and dental anomalies [2,3,5,7].

P3H1 may also be involved in tumor-related processes. In bladder urothelial carcinoma, its high expression was associated with poor prognosis, and high-expression samples had higher immune cell infiltration and enhanced sensitivity to several drugs [4]. Pan-cancer analysis showed that high P3H1 expression was significantly associated with low overall survival in multiple types of cancer [6].

In conclusion, P3h1 is essential for collagen processing, and its malfunction is closely related to OI. Additionally, its role in tumor development, as revealed by various research models, indicates its importance in understanding both skeletal and cancer-related diseases. The study of P3h1 knockout models in zebrafish and human genetic studies of P3H1 mutations have provided valuable insights into its functions in normal biological processes and disease conditions.

References:
1. Li, Wenguo, Peng, Junjiang, Yao, Deqiang, Zhao, Jie, Cao, Yu. 2024. The structural basis for the collagen processing by human P3H1/CRTAP/PPIB ternary complex. In Nature communications, 15, 7844. doi:10.1038/s41467-024-52321-6. https://pubmed.ncbi.nlm.nih.gov/39245686/
2. Kantaputra, Piranit Nik, Dejkhamron, Prapai, Intachai, Worrachet, Tongsima, Sissades, Angkurawaranon, Salita. 2021. A novel P3H1 mutation is associated with osteogenesis imperfecta type VIII and dental anomalies. In Oral surgery, oral medicine, oral pathology and oral radiology, 132, e198-e207. doi:10.1016/j.oooo.2021.01.023. https://pubmed.ncbi.nlm.nih.gov/33737016/
3. Kantaputra, Piranit Nik, Angkurawaranon, Salita, Intachai, Worrachet, Cox, Timothy C, Ketudat Cairns, James R. 2023. A Founder Intronic Variant in P3H1 Likely Results in Aberrant Splicing and Protein Truncation in Patients of Karen Descent with Osteogenesis Imperfecta Type VIII. In Genes, 14, . doi:10.3390/genes14020322. https://pubmed.ncbi.nlm.nih.gov/36833249/
4. Zhang, Yuanfeng, Chen, Yang, Chen, Zhiming, Wang, Zhiping, Zhang, Yonghai. 2023. Identification of P3H1 as a Predictive Prognostic Biomarker for Bladder Urothelial Carcinoma Based on the Cancer Genome Atlas Database. In Pharmacogenomics and personalized medicine, 16, 1041-1053. doi:10.2147/PGPM.S437974. https://pubmed.ncbi.nlm.nih.gov/38058295/
5. Bala, Mehmet Murat, Bala, Keziban Aslı. . Severe cases of osteogenesis imperfecta type VIII due to a homozygous mutation in P3H1 (LEPRE1) and review of the literature. In Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 30, 1233-1238. doi:10.17219/acem/141367. https://pubmed.ncbi.nlm.nih.gov/34637196/
6. Li, Yongjie, Wang, Ting, Jiang, Feng. 2024. Pan-Cancer Analysis of P3H1 and Experimental Validation in Renal Clear Cell Carcinoma. In Applied biochemistry and biotechnology, 196, 5974-5993. doi:10.1007/s12010-023-04845-8. https://pubmed.ncbi.nlm.nih.gov/38175417/
7. Zhytnik, Lidiia, Duy, Binh Ho, Eekhoff, Marelise, Maasalu, Katre, Micha, Dimitra. 2022. Phenotypic Variation in Vietnamese Osteogenesis Imperfecta Patients Sharing a Recessive P3H1 Pathogenic Variant. In Genes, 13, . doi:10.3390/genes13030407. https://pubmed.ncbi.nlm.nih.gov/35327962/
8. Tonelli, F, Cotti, S, Leoni, L, Witten, P E, Forlino, A. 2020. Crtap and p3h1 knock out zebrafish support defective collagen chaperoning as the cause of their osteogenesis imperfecta phenotype. In Matrix biology : journal of the International Society for Matrix Biology, 90, 40-60. doi:10.1016/j.matbio.2020.03.004. https://pubmed.ncbi.nlm.nih.gov/32173581/
9. Wu, Jiawei, Zhang, Wenting, Xia, Li, Zeng, Naiyan, Zhou, Aiwu. 2019. Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations. In Cellular and molecular life sciences : CMLS, 76, 3899-3914. doi:10.1007/s00018-019-03102-8. https://pubmed.ncbi.nlm.nih.gov/30993352/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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