C57BL/6JCya-Clec7aem1flox/Cya
Common Name:
Clec7a-flox
Product ID:
S-CKO-12186
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Clec7a-flox
Strain ID
CKOCMP-56644-Clec7a-B6J-VB
Gene Name
Product ID
S-CKO-12186
Gene Alias
BGR; Clecsf12; beta-GR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clec7aem1flox/Cya mice (Catalog S-CKO-12186) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000184581
NCBI RefSeq
NM_001309637
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Clec7a, also known as Dectin-1, is a pattern recognition receptor involved in various biological processes. It is associated with the recognition of fungi and plays a role in innate immune responses, especially in processes related to phagocytosis and inflammation [3,7]. Clec7a can directly activate SYK, which is involved in intracellular signaling pathways [4].
In disease models, Clec7a has shown significant impacts. In an ischemic stroke mouse model, manipulating microglial Clec7a expression regulated microglial phagocytosis of synapses, with Clec7a knockdown alleviating injury by inhibiting pyroptosis and microglial activation, suggesting it may be a therapeutic target for stroke [1,5]. In a Parkinson's disease model, AAV-mediated specific knockdown of Clec7a in microglial alleviated motor deficits and dopaminergic neuron damage, restraining neuroinflammation [6]. In an acute kidney injury model, combined expression of Clec7a in M1 macrophages and depletion in M2 macrophages improved renal function, indicating its role in macrophage phenotype regulation during AKI [2].
In conclusion, Clec7a is crucial in processes like phagocytosis and inflammation. Gene knockout or knockdown studies in mouse models for diseases such as ischemic stroke, Parkinson's disease, and acute kidney injury have revealed its potential as a therapeutic target. These models help us understand how Clec7a contributes to disease pathogenesis, providing insights for developing new treatment strategies.
References:
1. Wan, Hanxi, He, Mengfan, Cheng, Chun, Tian, Li, Xiong, Lize. 2024. Clec7a Worsens Long-Term Outcomes after Ischemic Stroke by Aggravating Microglia-Mediated Synapse Elimination. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2403064. doi:10.1002/advs.202403064. https://pubmed.ncbi.nlm.nih.gov/39088351/
2. Wang, Yaqiong, Li, Xianzhe, Xu, Xialian, Shen, Bo, Ding, Xiaoqiang. 2022. Clec7a expression in inflammatory macrophages orchestrates progression of acute kidney injury. In Frontiers in immunology, 13, 1008727. doi:10.3389/fimmu.2022.1008727. https://pubmed.ncbi.nlm.nih.gov/36189317/
3. Ma, Jie, Zhou, Miao, Song, Zehe, Yin, Yulong, Yin, Jie. 2023. Clec7a drives gut fungus-mediated host lipid deposition. In Microbiome, 11, 264. doi:10.1186/s40168-023-01698-5. https://pubmed.ncbi.nlm.nih.gov/38007451/
4. Wang, Shoutang, Sudan, Raki, Peng, Vincent, Brioschi, Simone, Colonna, Marco. . TREM2 drives microglia response to amyloid-β via SYK-dependent and -independent pathways. In Cell, 185, 4153-4169.e19. doi:10.1016/j.cell.2022.09.033. https://pubmed.ncbi.nlm.nih.gov/36306735/
5. Li, Wei, Feng, Xiaoli, Zhang, Manyu, Zhao, Zhenqiang, Xia, Min. . CLEC7A Knockdown Alleviates Ischemic Stroke by Inhibiting Pyroptosis and Microglia Activation. In Journal of integrative neuroscience, 23, 219. doi:10.31083/j.jin2312219. https://pubmed.ncbi.nlm.nih.gov/39735961/
6. Chen, Xue-Yun, Feng, Si-Ning, Bao, Yin, Zhou, Yu-Xin, Ba, Fang. 2023. Identification of Clec7a as the therapeutic target of rTMS in alleviating Parkinson's disease: targeting neuroinflammation. In Biochimica et biophysica acta. Molecular basis of disease, 1869, 166814. doi:10.1016/j.bbadis.2023.166814. https://pubmed.ncbi.nlm.nih.gov/37495085/
7. Hsu, Amy P, Korzeniowska, Agnieszka, Aguilar, Cynthia C, Lionakis, Michail S, Holland, Steven M. 2022. Immunogenetics associated with severe coccidioidomycosis. In JCI insight, 7, . doi:10.1172/jci.insight.159491. https://pubmed.ncbi.nlm.nih.gov/36166305/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen