C57BL/6JCya-Dctn5em1flox/Cya
Common Name:
Dctn5-flox
Product ID:
S-CKO-12522
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dctn5-flox
Strain ID
CKOCMP-59288-Dctn5-B6J-VA
Gene Name
Product ID
S-CKO-12522
Gene Alias
4930427E12Rik; b2b315Clo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dctn5em1flox/Cya mice (Catalog S-CKO-12522) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033156
NCBI RefSeq
NM_021608
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Dctn5, a subunit of the dynein complex involved in docking motor proteins, plays a role in microtubule-dependent trafficking. This process is associated with immune response, and studies on Dctn5 can provide insights into its role in biological processes [1].
In the Chinese tongue sole, two transcript variants of Dctn5 (dctn5_tv1 and dctn5_tv2) were identified. Dctn5_tv1 was widely distributed, especially highly expressed in immune tissues, and could be up-regulated after Vibrio harveyi challenge. Recombinant Dctn5_tv1 also showed antimicrobial activity, suggesting its involvement in the immune response to bacterial invasion [1].
In cutaneous melanoma, low expression of Dctn5 was associated with favorable overall survival, indicating its potential as a prognostic biomarker [2].
In mice with targeted inactivation of Wwtr1, which leads to glomerulocystic kidney disease, the expression of Dctn5 was decreased in the kidneys, suggesting its possible role in maintaining renal cilia integrity [3].
In double-mutant mice with Pink1 ablation and A53T-SNCA overexpression, Dctn5 was up-regulated, reflecting changes in various cellular dynamics and DNA damage [4].
In ischemic stroke, Dctn5 was part of an 8-gene signature that was highly accurate for diagnosing the disease [5].
In an in vitro study on mental disorders, knockdown of Dctn5, a bipolar disorder susceptibility gene, disrupted neuronal network physiology [6]. Also, a single-nucleotide polymorphism related to Dctn5 was associated with exercise intervention dropout in sedentary adults with overweight or obesity and cardiometabolic disease, potentially through alterations in gene expression and metabolic pathways in skeletal muscle [7].
In conclusion, Dctn5 is involved in multiple biological processes such as immune response, cancer prognosis, renal cilia integrity, and neuronal network regulation. Studies using genetic models like gene-knockout or knockdown in different organisms have revealed its significance in various disease conditions including infectious diseases, cancer, kidney diseases, mental disorders, and exercise-related phenotypes.
References：
1. Wei, Min, Xu, Wen-Teng, Li, Kun-Ming, Zhao, Fa-Zhen, Chen, Song-Lin. 2018. Cloning, characterization and functional analysis of dctn5 in immune response of Chinese tongue sole (Cynoglossus semilaevis). In Fish & shellfish immunology, 77, 392-401. doi:10.1016/j.fsi.2018.04.007. https://pubmed.ncbi.nlm.nih.gov/29635065/
2. Wang, Qiaoqi, Wang, Xiangkun, Liang, Qian, Li, Dong, Pan, Fuqiang. 2018. Prognostic Value of Dynactin mRNA Expression in Cutaneous Melanoma. In Medical science monitor : international medical journal of experimental and clinical research, 24, 3752-3763. doi:10.12659/MSM.910566. https://pubmed.ncbi.nlm.nih.gov/29864111/
3. Hossain, Zakir, Ali, Safiah Mohamed, Ko, Hui Ling, Hong, Wanjin, Hunziker, Walter. 2007. Glomerulocystic kidney disease in mice with a targeted inactivation of Wwtr1. In Proceedings of the National Academy of Sciences of the United States of America, 104, 1631-6. doi:. https://pubmed.ncbi.nlm.nih.gov/17251353/
4. Gispert, Suzana, Brehm, Nadine, Weil, Jonas, Roeper, Jochen, Auburger, Georg. 2014. Potentiation of neurotoxicity in double-mutant mice with Pink1 ablation and A53T-SNCA overexpression. In Human molecular genetics, 24, 1061-76. doi:10.1093/hmg/ddu520. https://pubmed.ncbi.nlm.nih.gov/25296918/
5. Feng, Bing, Meng, Xinling, Zhou, Hui, Wang, Hao, Zou, Donghua. 2021. Identification of Dysregulated Mechanisms and Potential Biomarkers in Ischemic Stroke Onset. In International journal of general medicine, 14, 4731-4744. doi:10.2147/IJGM.S327594. https://pubmed.ncbi.nlm.nih.gov/34456585/
6. MacLaren, Erik J, Charlesworth, Paul, Coba, Marcelo P, Grant, Seth G N. 2011. Knockdown of mental disorder susceptibility genes disrupts neuronal network physiology in vitro. In Molecular and cellular neurosciences, 47, 93-9. doi:10.1016/j.mcn.2010.12.014. https://pubmed.ncbi.nlm.nih.gov/21440632/
7. Jiang, Rong, Collins, Katherine A, Huffman, Kim M, Siegler, Ilene C, Kraus, William E. . Genome-Wide Genetic Analysis of Dropout in a Controlled Exercise Intervention in Sedentary Adults With Overweight or Obesity and Cardiometabolic Disease. In Annals of behavioral medicine : a publication of the Society of Behavioral Medicine, 58, 363-374. doi:10.1093/abm/kaae011. https://pubmed.ncbi.nlm.nih.gov/38489667/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen