C57BL/6JCya-Ireb2em1flox/Cya
Common Name:
Ireb2-flox
Product ID:
S-CKO-12770
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ireb2-flox
Strain ID
CKOCMP-64602-Ireb2-B6J-VA
Gene Name
Product ID
S-CKO-12770
Gene Alias
D9Ertd85e; Irp2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ireb2em1flox/Cya mice (Catalog S-CKO-12770) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034843
NCBI RefSeq
NM_022655
Target Region
Exon 6~8
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Ireb2, also known as iron-responsive element binding protein 2, is a key regulator in iron metabolism. It binds to iron-responsive elements (IREs) in the untranslated regions of mRNAs involved in iron metabolism, thereby regulating the translation or stability of these mRNAs. This regulation is crucial for maintaining cellular iron homeostasis, which impacts various biological processes and is associated with multiple diseases [2,3,4,5,6].
In chronic obstructive pulmonary disease (COPD), m6A-modified circSAV1 forms an RNA-protein ternary complex with YTHDF1 and Ireb2 mRNA, facilitating the translation of Ireb2. Elevated Ireb2 protein levels disrupt iron homeostasis, leading to the accumulation of a labile iron pool and lipid peroxidation, which triggers ferroptosis in lung epithelial cells. Silencing circSAV1 or treating with deferoxamine blocks CS-induced ferroptosis of lung epithelial cells, attenuating COPD progression in mice [1]. In non-small-cell lung cancer, knockdown of Ireb2 notably weakens curcumin-induced anti-tumor effect and ferroptosis, suggesting Ireb2 is involved in curcumin-induced ferroptosis [4]. In colorectal cancer cells, miR-19a suppresses ferroptosis by negatively regulating Ireb2, as Ireb2 is a direct target of miR-19a [7].
In conclusion, Ireb2 is essential for maintaining iron homeostasis. Model-based research, especially in the context of COPD, non-small-cell lung cancer, and colorectal cancer, has revealed its role in ferroptosis-related disease mechanisms. Understanding Ireb2's function provides insights into the pathogenesis of these diseases and may offer potential therapeutic targets.
References:
1. Xia, Haibo, Wu, Yan, Zhao, Jing, Bian, Tao, Liu, Qizhan. 2023. N6-Methyladenosine-modified circSAV1 triggers ferroptosis in COPD through recruiting YTHDF1 to facilitate the translation of IREB2. In Cell death and differentiation, 30, 1293-1304. doi:10.1038/s41418-023-01138-9. https://pubmed.ncbi.nlm.nih.gov/36828914/
2. Zeng, Qiaoli, Chen, Qikang, Zou, Dehua, Wang, Yajun, Ma, Guoda. 2020. Different Associations Between the IREB2 Variants and Chronic Obstructive Pulmonary Disease Susceptibility. In Frontiers in genetics, 11, 598053. doi:10.3389/fgene.2020.598053. https://pubmed.ncbi.nlm.nih.gov/33304392/
3. Qin, Xia, Zhang, Jun, Wang, Bin, Zou, Zhen, Yu, Chao. 2021. Ferritinophagy is involved in the zinc oxide nanoparticles-induced ferroptosis of vascular endothelial cells. In Autophagy, 17, 4266-4285. doi:10.1080/15548627.2021.1911016. https://pubmed.ncbi.nlm.nih.gov/33843441/
4. Tang, Xin, Ding, Hui, Liang, Maoli, Zhang, Jing, Cao, Jie. 2021. Curcumin induces ferroptosis in non-small-cell lung cancer via activating autophagy. In Thoracic cancer, 12, 1219-1230. doi:10.1111/1759-7714.13904. https://pubmed.ncbi.nlm.nih.gov/33656766/
5. Zhu, Ting, Xiao, Zhuoyu, Yuan, Haoyu, Liu, Cundong, Zhou, Junhao. 2022. ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC. In Frontiers in oncology, 12, 929838. doi:10.3389/fonc.2022.929838. https://pubmed.ncbi.nlm.nih.gov/36059676/
6. Shao, Min, Cheng, Haipeng, Li, Xiaohong, Zhou, Yan, Luo, Ziqiang. 2024. Abnormal mitochondrial iron metabolism damages alveolar type II epithelial cells involved in bleomycin-induced pulmonary fibrosis. In Theranostics, 14, 2687-2705. doi:10.7150/thno.94072. https://pubmed.ncbi.nlm.nih.gov/38773980/
7. Fan, Hongwei, Ai, Rong, Mu, Suen, Guo, Zhengrong, Liu, Lin. . MiR-19a suppresses ferroptosis of colorectal cancer cells by targeting IREB2. In Bioengineered, 13, 12021-12029. doi:10.1080/21655979.2022.2054194. https://pubmed.ncbi.nlm.nih.gov/35599631/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen