C57BL/6JCya-Acer3em1flox/Cya
Common Name:
Acer3-flox
Product ID:
S-CKO-12932
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Acer3-flox
Strain ID
CKOCMP-66190-Acer3-B6J-VA
Gene Name
Product ID
S-CKO-12932
Gene Alias
1110057L18Rik; 5430429L08Rik; Phca; aPHC
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acer3em1flox/Cya mice (Catalog S-CKO-12932) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033020
NCBI RefSeq
NM_025408
Target Region
Exon 3~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Acer3, also known as alkaline ceramidase 3, is an enzyme that catalyzes the hydrolysis of ceramide to sphingosine. It is a member of the CREST superfamily of integral-membrane hydrolases and is a Zn2+-dependent amidase [4]. It plays a role in lipid metabolism, especially in sphingolipid metabolism, and is associated with multiple biological processes [3].
In acute myeloid leukemia (AML), in silico analysis showed that ACER3 expression inversely correlates with the overall survival of AML patients. Inhibition of acer3 expression in human AML cells using an shRNA-encoding lentivirus system led to decreased cell growth, colony formation, elevated apoptosis, and lower AKT signaling, suggesting that ACER3 contributes to AML pathogenesis [1].
In cholestatic liver injury (CLI), ACER3 expression is upregulated in the cholestatic liver and positively correlated with the severity of CLI in patients. Acer3 ablation in female mice increased ceramide(d18:1/18:1) and attenuated bile duct ligation-induced CLI with reduced hepatic necrosis, inflammation, and fibrosis, uncovering the role of ceramide(d18:1/18:1)-liver X receptor β signaling in mitigating bile acid overload [2].
In conclusion, Acer3 is crucial in lipid metabolism, especially sphingolipid metabolism. Its dysregulation is associated with diseases like AML and CLI. The use of gene-knockout models in these studies has provided insights into its role in disease pathogenesis, which can potentially guide the development of therapeutic strategies targeting Acer3 in these disease areas.
References：
1. Chen, Chen, Yin, Yancun, Li, Chunling, Wang, Yuesi, Zhang, Cheng Cheng. 2016. ACER3 supports development of acute myeloid leukemia. In Biochemical and biophysical research communications, 478, 33-38. doi:10.1016/j.bbrc.2016.07.099. https://pubmed.ncbi.nlm.nih.gov/27470583/
2. Liao, Leyi, Liu, Ziying, Liu, Lei, Li, Chuanjiang, Wang, Kai. 2025. Targeting the ceramidase ACER3 attenuates cholestasis in mice by mitigating bile acid overload via unsaturated ceramide-mediated LXRβ signaling transduction. In Nature communications, 16, 2112. doi:10.1038/s41467-025-57330-7. https://pubmed.ncbi.nlm.nih.gov/40025008/
3. Xu, Ruijuan, Antwi Boasiako, Paul, Mao, Cungui. 2020. Alkaline ceramidase family: The first two decades. In Cellular signalling, 78, 109860. doi:10.1016/j.cellsig.2020.109860. https://pubmed.ncbi.nlm.nih.gov/33271224/
4. Yi, Jae Kyo, Xu, Ruijuan, Obeid, Lina M, Airola, Michael V, Mao, Cungui. 2022. Alkaline ceramidase catalyzes the hydrolysis of ceramides via a catalytic mechanism shared by Zn2+-dependent amidases. In PloS one, 17, e0271540. doi:10.1371/journal.pone.0271540. https://pubmed.ncbi.nlm.nih.gov/36048828/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen