C57BL/6JCya-Oxct1em1flox/Cya
Common Name:
Oxct1-flox
Product ID:
S-CKO-13466
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Oxct1-flox
Strain ID
CKOCMP-67041-Oxct1-B6J-VA
Gene Name
Product ID
S-CKO-13466
Gene Alias
2610008O03Rik; Oxct; Oxct2a; SCOT; Scot-s
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Oxct1em1flox/Cya mice (Catalog S-CKO-13466) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000110690
NCBI RefSeq
NM_024188
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
OXCT1, also known as 3-oxoacid CoA-transferase 1, is a key enzyme in ketone body metabolism. It catalyzes the first and rate-determining step of ketolysis, converting the product to acetyl-CoA which enters the tricarboxylic acid cycle for energy production [3]. OXCT1 is involved in various biological processes, such as adipogenesis, neurogenesis, and is also linked to metabolic reprogramming in cancer cells [3,4,5].
In hepatocellular carcinoma (HCC), OXCT1 has been extensively studied. In LysMcreOXCT1f/f (OXCT1 conditional knockout in macrophages) mice, inhibiting OXCT1 expression in tumor-associated macrophages reduced CD8+ T-cell exhaustion through the succinate-H3K4me3-Arg1 axis, suppressing tumor growth by reprogramming TAMs toward an antitumor phenotype [1]. In another study, in HCC cells, SUCLA2-mediated OXCT1 succinylation and activation enhanced ketolysis, cell proliferation, and tumor growth in mice, and an OXCT1 inhibitor could suppress liver tumor growth [2]. In pancreatic ductal adenocarcinoma, down-regulation of OXCT1 in cell lines increased apoptosis after gemcitabine treatment, and overexpression of OXCT1 in mouse models reduced the tumor-growth-inhibitory effect of gemcitabine, suggesting OXCT1 promotes gemcitabine resistance via the NF-κB signaling pathway [6].
In conclusion, OXCT1 is essential for ketone body metabolism and plays significant roles in multiple biological processes and diseases. The use of gene knockout and conditional knockout mouse models, such as in HCC and pancreatic cancer studies, has revealed its functions in tumor-related processes, providing potential therapeutic targets for these diseases.
References:
1. Zhu, Chu-Xu, Yan, Kai, Chen, Liang, Gao, Ping, Lian, Zhe-Xiong. 2024. Targeting OXCT1-mediated ketone metabolism reprograms macrophages to promote antitumor immunity via CD8+ T cells in hepatocellular carcinoma. In Journal of hepatology, 81, 690-703. doi:10.1016/j.jhep.2024.05.007. https://pubmed.ncbi.nlm.nih.gov/38759889/
2. Guo, Dong, Yu, Qiujing, Tong, Yingying, Yan, Dong, Lu, Zhimin. 2025. OXCT1 succinylation and activation by SUCLA2 promotes ketolysis and liver tumor growth. In Molecular cell, 85, 843-856.e6. doi:10.1016/j.molcel.2024.12.025. https://pubmed.ncbi.nlm.nih.gov/39862868/
3. Zhang, Song, Xie, Caifeng. 2017. The role of OXCT1 in the pathogenesis of cancer as a rate-limiting enzyme of ketone body metabolism. In Life sciences, 183, 110-115. doi:10.1016/j.lfs.2017.07.003. https://pubmed.ncbi.nlm.nih.gov/28684065/
4. Zeng, Jie, Zhou, Shi-Wei, Zhao, Jin, Wang, Xiao-Long, Chen, Yu-Lin. 2019. Role of OXCT1 in ovine adipose and preadipocyte differentiation. In Biochemical and biophysical research communications, 512, 779-785. doi:10.1016/j.bbrc.2019.03.128. https://pubmed.ncbi.nlm.nih.gov/30928098/
5. Qiu, Jia-Yin, Gao, Sheng-Qing, Chen, Yu-Sheng, Sun, Yan, Zhou, Meng-Liang. 2024. OXCT1 regulates hippocampal neurogenesis and alleviates cognitive impairment via the Akt/GSK-3β/β-catenin pathway after subarachnoid hemorrhage. In Brain research, 1827, 148758. doi:10.1016/j.brainres.2024.148758. https://pubmed.ncbi.nlm.nih.gov/38199308/
6. Ding, Jinsheng, Li, Hui, Liu, Yang, Wang, Hongwei, Gao, Chuntao. 2021. OXCT1 Enhances Gemcitabine Resistance Through NF-κB Pathway in Pancreatic Ductal Adenocarcinoma. In Frontiers in oncology, 11, 698302. doi:10.3389/fonc.2021.698302. https://pubmed.ncbi.nlm.nih.gov/34804914/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen