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C57BL/6JCya-Mis12em1flox/Cya
Common Name:
Mis12-flox
Product ID:
S-CKO-13528
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mis12-flox
Strain ID
CKOCMP-67139-Mis12-B6J-VA
Gene Name
Mis12
Product ID
S-CKO-13528
Gene Alias
2510025F08Rik; Misc12
Background
C57BL/6JCya
NCBI ID
67139
Modification
Conditional knockout
Chromosome
11
Phenotype
MGI:1914389
Document
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Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mis12em1flox/Cya mice (Catalog S-CKO-13528) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048807
NCBI RefSeq
NM_025993
Target Region
Exon 3
Size of Effective Region
~3.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mis12 is a gene encoding a protein that plays crucial roles in cell division and cellular senescence. It is part of the Mis12 complex, which is a key component of the kinetochore, a structure essential for the attachment of chromosomes to spindle microtubules during mitosis [3,5,6]. Additionally, Mis12 is involved in regulating pathways related to cell cycle transitions and senescence, making it of great biological importance. Genetic models, such as mouse models, are valuable for studying its functions.

In mouse oocytes, depletion of Mis12 severely compromises germinal vesicle breakdown (GVBD) by impairing cyclin B1 accumulation, indicating its requirement for meiotic G2/M transition. However, it is dispensable for meiotic progression through meiosis I and II [1]. In prematurely senescent human mesenchymal stem cells, knockout of METTL3, which normally stabilizes MIS12 mRNA via m6A modifications, accelerates cellular senescence, suggesting that Mis12 is important in counteracting premature aging [2]. Also, in atherosclerotic plaque development, FTO stabilizes the MIS12 protein in vascular smooth muscle cells, inhibiting their senescence and thus slowing the progression of atherosclerotic plaques [4].

In conclusion, Mis12 is essential for cell cycle regulation during meiosis and in preventing cellular senescence. The gene knockout studies in mouse models and human cells have revealed its significance in diseases related to premature aging and atherosclerosis. Understanding Mis12's functions provides insights into the mechanisms underlying these biological processes and diseases, which may potentially lead to new therapeutic strategies.

References:
1. Bai, Guang-Yu, Choe, Min Ho, Kim, Jae-Sung, Oh, Jeong Su. 2020. Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/CCdh1 regulation during meiotic G2/M transition in mouse oocytes. In Development (Cambridge, England), 147, . doi:10.1242/dev.185322. https://pubmed.ncbi.nlm.nih.gov/32341029/
2. Wu, Zeming, Shi, Yue, Lu, Mingming, Ci, Weimin, Qu, Jing. . METTL3 counteracts premature aging via m6A-dependent stabilization of MIS12 mRNA. In Nucleic acids research, 48, 11083-11096. doi:10.1093/nar/gkaa816. https://pubmed.ncbi.nlm.nih.gov/33035345/
3. Ladurner, Rene, Straight, Aaron F. . MIS12/MIND Control at the Kinetochore. In Cell, 167, 889-891. doi:10.1016/j.cell.2016.10.036. https://pubmed.ncbi.nlm.nih.gov/27814517/
4. Sun, Jingzhao, Wang, Mengqi, Jia, Fengming, Ren, Jinlin, Hu, Bo. 2024. FTO Stabilizes MIS12 to Inhibit Vascular Smooth Muscle Cell Senescence in Atherosclerotic Plaque. In Journal of inflammation research, 17, 1857-1871. doi:10.2147/JIR.S447379. https://pubmed.ncbi.nlm.nih.gov/38523689/
5. Petrovic, Arsen, Pasqualato, Sebastiano, Dube, Prakash, Stark, Holger, Musacchio, Andrea. . The MIS12 complex is a protein interaction hub for outer kinetochore assembly. In The Journal of cell biology, 190, 835-52. doi:10.1083/jcb.201002070. https://pubmed.ncbi.nlm.nih.gov/20819937/
6. Petrovic, Arsen, Keller, Jenny, Liu, Yahui, Vetter, Ingrid R, Musacchio, Andrea. 2016. Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores. In Cell, 167, 1028-1040.e15. doi:10.1016/j.cell.2016.10.005. https://pubmed.ncbi.nlm.nih.gov/27881301/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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