C57BL/6JCya-Ddx47em1flox/Cya
Common Name:
Ddx47-flox
Product ID:
S-CKO-13860
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ddx47-flox
Strain ID
CKOCMP-67755-Ddx47-B6J-VA
Gene Name
Product ID
S-CKO-13860
Gene Alias
4930588A18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ddx47em1flox/Cya mice (Catalog S-CKO-13860) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032326
NCBI RefSeq
NM_026360
Target Region
Exon 3~5
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Ddx47, also known as DEAD-box helicase 47, is a crucial helicase involved in RNA processing, especially in resolving R-loops, which are transient structures formed during gene transcription and a threat to genome stability [2,3,6]. It is also associated with processes like cell division, ribosome biogenesis, and tissue homeostasis [1,4,5].
In lung adenocarcinoma, Ddx47 is highly expressed, and its high expression predicts a poor prognosis. Knockdown of Ddx47 reduces cell proliferation and migration in vitro, confirming its cancer-promoting effect [1]. In locusts, knockdown of LmDDX47 (the ortholog of Ddx47) leads to midgut atrophy, reduction in regenerative and columnar cells, and induction of cell death in columnar tissue, suggesting its role in maintaining midgut homeostasis [4].
In conclusion, Ddx47 plays essential roles in RNA processing, cell proliferation, migration, and tissue homeostasis. Its study through knockdown models in different organisms reveals its significance in cancer development and tissue-specific functions, providing insights into potential therapeutic targets for diseases like lung adenocarcinoma.
References：
1. Chen, Yang, Zeng, Lizhong, Wang, Wei, Sun, Ruiying, Yang, Shuanying. 2022. DDX47 promotes cell proliferation and migration in lung adenocarcinoma. In Pathology, research and practice, 234, 153921. doi:10.1016/j.prp.2022.153921. https://pubmed.ncbi.nlm.nih.gov/35490652/
2. Yu, Zhenbao, Richard, Stéphane. 2023. DDX47 untangles R-loops with only certain other helicases. In Trends in cell biology, 33, 359-360. doi:10.1016/j.tcb.2023.03.001. https://pubmed.ncbi.nlm.nih.gov/36914533/
3. Marchena-Cruz, Esther, Camino, Lola P, Bhandari, Jay, Luna, Rosa, Aguilera, Andrés. 2023. DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops. In Cell reports, 42, 112148. doi:10.1016/j.celrep.2023.112148. https://pubmed.ncbi.nlm.nih.gov/36827184/
4. Wang, Jun-Xiu, Ma, En-Bo, Zhang, Jian-Zhen, Xing, Shu-Ping. 2022. DEAD-Box RNA Helicase DDX47 Maintains Midgut Homeostasis in Locusta migratoria. In International journal of molecular sciences, 23, . doi:10.3390/ijms23020586. https://pubmed.ncbi.nlm.nih.gov/35054771/
5. Sekiguchi, Takeshi, Hayano, Toshiya, Yanagida, Mitsuaki, Takahashi, Nobuhiro, Nishimoto, Takeharu. 2006. NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47. In Nucleic acids research, 34, 4593-608. doi:. https://pubmed.ncbi.nlm.nih.gov/16963496/
6. Okamoto, Yusuke, Abe, Masako, Itaya, Akiko, Isobe, Toshiaki, Takata, Minoru. 2018. FANCD2 protects genome stability by recruiting RNA processing enzymes to resolve R-loops during mild replication stress. In The FEBS journal, 286, 139-150. doi:10.1111/febs.14700. https://pubmed.ncbi.nlm.nih.gov/30431240/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen