C57BL/6JCya-Derl1em1flox/Cya
Common Name:
Derl1-flox
Product ID:
S-CKO-13893
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Derl1-flox
Strain ID
CKOCMP-67819-Derl1-B6J-VA
Gene Name
Product ID
S-CKO-13893
Gene Alias
1110021N07Rik; Derlin-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Derl1em1flox/Cya mice (Catalog S-CKO-13893) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022993
NCBI RefSeq
NM_024207
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Derl1, also known as derlin 1, is an important protein involved in the endoplasmic reticulum-associated degradation (ERAD) pathway. It participates in the retrotranslocation and degradation of misfolded proteins from the endoplasmic reticulum (ER), which is crucial for maintaining protein homeostasis within the cell [6].
Derl1 has been implicated in multiple diseases. In triple-negative breast cancer (TNBC), it is part of the VCP-TMEM63A-DERL1 signaling axis. Pharmacological inhibition of VCP or knockdown of DERL1 can partially abolish the oncogenic effects of TMEM63A on TNBC progression, suggesting its role in promoting TNBC cell proliferation, migration, and invasion [1]. In breast cancer, overexpression of DERL1 promotes p-AKT activation through K63 ubiquitination, and knockdown of DERL1 inhibits breast cancer cell growth both in vitro and in vivo [2]. In non-small-cell lung cancer, TEAD1 transcriptionally activates DERL1, and the circTTBK2/miR-873-5p/TEAD1/DERL1 axis is involved in tumorigenesis [3]. In oral squamous cell carcinoma (OSCC), DERL1 is involved in resistance to cisplatin-induced cell death, and melatonin can enhance cisplatin-induced cell death by inhibiting DERL1 [4]. In hepatocellular carcinoma (HCC), DERL1 promotes tumor progression via the AKT pathway, and its high expression is associated with larger tumor diameters and lymph node metastases [5]. In non-alcoholic fatty liver disease (NAFLD), Derlin-1 can both promote the disease by increasing RIPK3-mediated necroptosis [7] and ameliorate nonalcoholic hepatic steatosis by promoting ubiquitylation and degradation of FABP1 [9]. In pulmonary arterial hypertension, DERL1 is identified as one of the top 5 hub genes related to ER stress, and these hub genes are closely related to immune cells [8].
In conclusion, Derl1 plays essential roles in protein quality control through the ERAD pathway. Its dysregulation is associated with multiple diseases, including various cancers and metabolic diseases. Studies using knockdown or inhibition of Derl1 in different in vitro and in vivo models have revealed its oncogenic or disease-promoting roles, highlighting its potential as a therapeutic target for these diseases.
References：
1. Zhang, Tai-Mei, Liao, Li, Yang, Shao-Ying, Shao, Zhi-Min, Li, Da-Qiang. 2022. TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression. In Autophagy, 19, 805-821. doi:10.1080/15548627.2022.2103992. https://pubmed.ncbi.nlm.nih.gov/35920704/
2. Bai, Yang, Zhang, Zhanqiang, Bi, Jiong, Yao, Chen, Wang, Wenjian. 2024. miR-181c-5p/DERL1 pathway controls breast cancer progression mediated by TRAF6-linked K63 ubiquitination of AKT. In Cancer cell international, 24, 204. doi:10.1186/s12935-024-03395-1. https://pubmed.ncbi.nlm.nih.gov/38858669/
3. Wei, Jin-Ying, Zhang, Qiang, Yao, Yue, Meng, Guang-Ping, Zhang, Jie. 2022. Circular RNA circTTBK2 facilitates non-small-cell lung cancer malignancy through the miR-873-5p/TEAD1/DERL1 axis. In Epigenomics, 14, 931-949. doi:10.2217/epi-2021-0480. https://pubmed.ncbi.nlm.nih.gov/35916080/
4. Shigeishi, Hideo, Yokoyama, Sho, Murodumi, Hiroshi, Takechi, Masaaki, Ohta, Kouji. 2021. Melatonin enhances cisplatin-induced cell death through inhibition of DERL1 in mesenchymal-like CD44high OSCC cells. In Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 51, 281-289. doi:10.1111/jop.13242. https://pubmed.ncbi.nlm.nih.gov/34551150/
5. Fan, Jiye, Tian, Liying, Huang, Shuhong, Zhang, Jing, Zhao, Baohua. 2020. Derlin-1 Promotes the Progression of Human Hepatocellular Carcinoma via the Activation of AKT Pathway. In OncoTargets and therapy, 13, 5407-5417. doi:10.2147/OTT.S222895. https://pubmed.ncbi.nlm.nih.gov/32606758/
6. Ndiaye, Kalidou, Lussier, Jacques G, Pate, Joy L. 2010. Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea. In Reproductive biology and endocrinology : RB&E, 8, 94. doi:10.1186/1477-7827-8-94. https://pubmed.ncbi.nlm.nih.gov/20682045/
7. Wang, Ting, Wang, Dehua, Kuang, Ge, Wan, Jingyuan, Li, Ke. 2024. Derlin-1 promotes diet-induced non-alcoholic fatty liver disease via increasing RIPK3-mediated necroptosis. In Free radical biology & medicine, 217, 29-47. doi:10.1016/j.freeradbiomed.2024.03.014. https://pubmed.ncbi.nlm.nih.gov/38522486/
8. Yang, Qi, Lai, Banghui, Xie, Hao, Liao, Bin, Liu, Feng. 2024. Identification of differentially expressed ER stress-related genes and their association with pulmonary arterial hypertension. In Respiratory research, 25, 220. doi:10.1186/s12931-024-02849-4. https://pubmed.ncbi.nlm.nih.gov/38789967/
9. You, Hui, Wen, Xin, Wang, Xingchun, Zhang, Jun, Qu, Shen. 2023. Derlin-1 ameliorates nonalcoholic hepatic steatosis by promoting ubiquitylation and degradation of FABP1. In Free radical biology & medicine, 207, 260-271. doi:10.1016/j.freeradbiomed.2023.07.026. https://pubmed.ncbi.nlm.nih.gov/37499886/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen