C57BL/6JCya-Puf60em1flox/Cya
Common Name:
Puf60-flox
Product ID:
S-CKO-13987
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Puf60-flox
Strain ID
CKOCMP-67959-Puf60-B6J-VA
Gene Name
Product ID
S-CKO-13987
Gene Alias
2410104I19Rik; 2810454F19Rik; SIAHBP1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Puf60em1flox/Cya mice (Catalog S-CKO-13987) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100527
NCBI RefSeq
NM_028364
Target Region
Exon 6~12
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
PUF60, also known as Poly(U) binding splicing factor 60 kDa, is a nucleic acid-binding protein involved in multiple nuclear processes. It is essential for embryonic development, regulating gene expression and cellular growth through pre-mRNA splicing, apoptosis, and transcription regulation [2,4].
PUF60 depletion inhibits the G2/M transition of the cell cycle in lung adenocarcinoma cells, leading to reduced cell proliferation and tumor development. This is because PUF60 knockdown causes exon skipping in genes like CDC25C, resulting in nonsense-mediated mRNA decay and decreased protein levels [1].
In glioblastoma, PUF60 knockdown significantly decreases cell proliferation in vitro and in vivo by reducing the ubiquitination level of EGFR through transcriptional regulation of STUB1 [3].
In bladder cancer, PUF60 knockdown inhibits cell viability and colony formation, while its overexpression promotes these processes, and it activates the transcription of AURKA [5].
In breast cancer, upregulation of PUF60 accelerates cancer progression by downregulating PTEN expression [6].
In ovarian cancer, silencing PUF60 enhances the stability of mRNA transcripts involved in oxidative phosphorylation, reducing tumor cell proliferation and migration [7].
In conclusion, PUF60 is crucial for normal development and its dysregulation is involved in multiple cancers. Loss-of-function studies in various cancer cell models have revealed its role in promoting tumor progression through different molecular mechanisms, suggesting it could be a potential therapeutic target for cancer treatment.
References：
1. Xu, Nan, Ren, Yunpeng, Bao, Yufang, Xie, Gangcai, Wang, Yongbo. 2023. PUF60 promotes cell cycle and lung cancer progression by regulating alternative splicing of CDC25C. In Cell reports, 42, 113041. doi:10.1016/j.celrep.2023.113041. https://pubmed.ncbi.nlm.nih.gov/37682709/
2. Hoogenboom, Amarens, Falix, Farah A, van der Laan, Liselot, Sadikovic, Bekim, van Haelst, Mieke M. 2024. Novel PUF60 variant suggesting an interaction between Verheij and Cornelia de Lange syndrome: phenotype description and review of the literature. In European journal of human genetics : EJHG, 32, 435-439. doi:10.1038/s41431-023-01527-1. https://pubmed.ncbi.nlm.nih.gov/38273166/
3. Wang, Fang, Peng, Lan, Sun, Yikui, Zhang, Burong, Lu, Shuaijun. 2022. PUF60 promotes glioblastoma progression through regulation of EGFR stability. In Biochemical and biophysical research communications, 636, 190-196. doi:10.1016/j.bbrc.2022.10.082. https://pubmed.ncbi.nlm.nih.gov/36335869/
4. Bach, Michal Yacobi, Miron, Sivan Reytan, Kurolap, Alina, Feldman, Hagit Baris. 2024. PUF60 loss-of-function with normal cognition should be considered in the differential diagnosis of Klippel-Feil syndrome. In American journal of medical genetics. Part A, 194, e63550. doi:10.1002/ajmg.a.63550. https://pubmed.ncbi.nlm.nih.gov/38297485/
5. Long, Qian, An, Xin, Chen, Miao, Zheng, Fufu, He, Liru. 2020. PUF60/AURKA Axis Contributes to Tumor Progression and Malignant Phenotypes in Bladder Cancer. In Frontiers in oncology, 10, 568015. doi:10.3389/fonc.2020.568015. https://pubmed.ncbi.nlm.nih.gov/33117697/
6. Sun, Dongying, Lei, Wei, Hou, Xiaodong, Li, Hui, Ni, Wenlu. 2019. PUF60 accelerates the progression of breast cancer through downregulation of PTEN expression. In Cancer management and research, 11, 821-830. doi:10.2147/CMAR.S180242. https://pubmed.ncbi.nlm.nih.gov/30697074/
7. Zhang, Cancan, Ni, Xiaoge, Tao, Chunlin, Wu, Zhiyong, Zhang, Rong. 2023. Targeting PUF60 prevents tumor progression by retarding mRNA decay of oxidative phosphorylation in ovarian cancer. In Cellular oncology (Dordrecht, Netherlands), 47, 157-174. doi:10.1007/s13402-023-00859-w. https://pubmed.ncbi.nlm.nih.gov/37632669/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen