C57BL/6JCya-Nudt12em1flox/Cya
Common Name:
Nudt12-flox
Product ID:
S-CKO-14002
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nudt12-flox
Strain ID
CKOCMP-67993-Nudt12-B6J-VA
Gene Name
Product ID
S-CKO-14002
Gene Alias
0610016O18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nudt12em1flox/Cya mice (Catalog S-CKO-14002) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025065
NCBI RefSeq
NM_026497
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Nudt12, a Nudix hydrolase, is involved in RNA cap metabolism, particularly in the turnover of metabolite-capped RNAs such as NAD-capped RNAs. It also hydrolyzes NAD(P)H and other related compounds [4]. It may play a role in regulating peroxisomal nicotinamide nucleotide cofactors for oxidative metabolism [4].
Loss of Nudt12 results in a significant upregulation of circadian clock transcripts in mouse liver, pointing to a physiological role in the cytosolic surveillance of NAD-RNAs [1]. Disruption of the Nudt12 gene stabilizes transfected NAD-capped RNA in cells, and its endogenous NAD-capped mRNA targets are enriched in those encoding proteins involved in cellular energetics. Also, exposure to nutrient or environmental stress shows changes in NAD-capped RNA levels selectively responsive to Nudt12, indicating an association with cellular metabolism [2].
In conclusion, Nudt12 is crucial for the metabolism of metabolite-capped RNAs, especially NAD-capped RNAs. Studies with Nudt12-disrupted mouse models have revealed its role in regulating circadian clock transcripts and its association with cellular metabolism, which may provide insights into understanding related biological processes and disease mechanisms, such as its potential involvement in diabetes-related neuropathy as suggested by biomarker-based studies [1,2,3].
References：
1. Wu, Hao, Li, Lingyun, Chen, Kuan-Ming, McCarthy, Andrew A, Pillai, Ramesh S. . Decapping Enzyme NUDT12 Partners with BLMH for Cytoplasmic Surveillance of NAD-Capped RNAs. In Cell reports, 29, 4422-4434.e13. doi:10.1016/j.celrep.2019.11.108. https://pubmed.ncbi.nlm.nih.gov/31875550/
2. Grudzien-Nogalska, Ewa, Wu, Yixuan, Jiao, Xinfu, Tong, Liang, Kiledjian, Megerditch. 2019. Structural and mechanistic basis of mammalian Nudt12 RNA deNADding. In Nature chemical biology, 15, 575-582. doi:10.1038/s41589-019-0293-7. https://pubmed.ncbi.nlm.nih.gov/31101919/
3. Ye, Chenhao, Fu, Yuedong, Zhou, Xijie, Zhu, Xuwei, Chen, Yiheng. 2024. Identification and validation of NAD+ metabolism-related biomarkers in patients with diabetic peripheral neuropathy. In Frontiers in endocrinology, 15, 1309917. doi:10.3389/fendo.2024.1309917. https://pubmed.ncbi.nlm.nih.gov/38464965/
4. Abdelraheim, Salama R, Spiller, David G, McLennan, Alexander G. . Mammalian NADH diphosphatases of the Nudix family: cloning and characterization of the human peroxisomal NUDT12 protein. In The Biochemical journal, 374, 329-35. doi:. https://pubmed.ncbi.nlm.nih.gov/12790796/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen