C57BL/6JCya-Rragaem1flox/Cya
Common Name:
Rraga-flox
Product ID:
S-CKO-14173
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rraga-flox
Strain ID
CKOCMP-68441-Rraga-B6J-VA
Gene Name
Product ID
S-CKO-14173
Gene Alias
1300010C19Rik; FIP-1; RAGA
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rragaem1flox/Cya mice (Catalog S-CKO-14173) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000091064
NCBI RefSeq
NM_178376
Target Region
Exon 1
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
RRAGA, which encodes Ras-related GTP-binding protein A (RagA), is a key player in the mTORC1 pathway [1,2,3,4,5,6]. RagA initially senses cellular amino acids (e.g., leucine) and controls mTORC1's translocation to the lysosomal membrane, a process crucial for cell growth regulation in response to various environmental cues such as nutrients, growth factors, and energy/oxygen status [1,6]. The mTORC1 pathway, in which RRAGA is involved, has far-reaching implications for cell growth and metabolism [4]. Genetic models, like gene knockout models, are valuable for studying RRAGA's functions.
A RRAGA knockout human iPSC line was generated using Nuclease technology technology, which may aid in developing new therapeutics for depression as RagA overexpression seems linked to depression onset [1]. In human lens epithelial cells, mutations in RRAGA associated with autosomal dominant cataracts disrupted mTORC1 signaling, including increased RRAGA relocation to lysosomes, up-regulated mTORC1 phosphorylation, down-regulated autophagy, and altered cell growth [5].
In summary, RRAGA is essential for the mTORC1-mediated amino acid sensing and cell growth regulation. Gene knockout models of RRAGA have provided insights into its role in conditions like depression and autosomal dominant cataracts, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Sun, Yilu, Fu, Jian, Yang, Jiayin, Zhao, Jia, Rong, Jianhui. 2022. Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using Nuclease technology technology. In Stem cell research, 63, 102859. doi:10.1016/j.scr.2022.102859. https://pubmed.ncbi.nlm.nih.gov/35870248/
2. Liu, Kunpeng, Qiu, Dongbo, Liang, Xue, Qin, Yunfei, Zhang, Qi. 2021. Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy. In Autophagy, 18, 860-876. doi:10.1080/15548627.2021.1961072. https://pubmed.ncbi.nlm.nih.gov/34382907/
3. Jia, Jingyue, Wang, Fulong, Bhujabal, Zambarlal, Johansen, Terje, Deretic, Vojo. 2022. Membrane Atg8ylation, stress granule formation, and MTOR regulation during lysosomal damage. In Autophagy, 19, 1893-1895. doi:10.1080/15548627.2022.2148900. https://pubmed.ncbi.nlm.nih.gov/36394332/
4. Valenstein, Max L, Lalgudi, Pranav V, Gu, Xin, Chivukula, Raghu R, Sabatini, David M. 2024. Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2322755121. doi:10.1073/pnas.2322755121. https://pubmed.ncbi.nlm.nih.gov/39163330/
5. Chen, Jian-Huan, Huang, Chukai, Zhang, Bining, Pang, Chi-Pui, Zhang, Mingzhi. 2016. Mutations of RagA GTPase in mTORC1 Pathway Are Associated with Autosomal Dominant Cataracts. In PLoS genetics, 12, e1006090. doi:10.1371/journal.pgen.1006090. https://pubmed.ncbi.nlm.nih.gov/27294265/
6. Kim, Joungmok, Kim, Eunjung. 2016. Rag GTPase in amino acid signaling. In Amino acids, 48, 915-928. doi:10.1007/s00726-016-2171-x. https://pubmed.ncbi.nlm.nih.gov/26781224/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen