C57BL/6JCya-Taf11em1flox/Cya
Common Name
Taf11-flox
Product ID
S-CKO-14301
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-68776-Taf11-B6J-VA
Status
When using this mouse strain in a publication, please cite “Taf11-flox Mouse (Catalog S-CKO-14301) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Taf11-flox
Strain ID
CKOCMP-68776-Taf11-B6J-VA
Gene Name
Product ID
S-CKO-14301
Gene Alias
TAF2I, PRO214, TAFII28, 1110038O14Rik
Background
C57BL/6JCya
Gene Full Name
TATA-box binding protein associated factor 11
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 17 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000114848
NCBI Transcript ID
NM_026836
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Overview of Gene Research
Taf11, also known as TATA-binding protein-associated factor 11, is involved in multiple biological processes. It is an annotated nuclear transcription factor and plays a role in the assembly of the RNA-induced silencing complex (RLC), enhancing RNAi efficiency [1]. Taf11 also interacts with other transcription-related factors such as TFIIA, and is part of the general transcription factor TFIID complex, which is crucial for RNA polymerase II transcription initiation [3,4].
In Drosophila, taf11 knockout in ovary extract led to defective RLC assembly, and reconstitution of RLC in vitro using recombinant Dcr-2-R2D2 complex, TAF11, and duplex siRNA demonstrated that TAF11 tetramer facilitates Dcr-2-R2D2 tetramerization to enhance siRNA binding and RISC loading activities [1]. In zebrafish, overexpression of TAF11 and taf11 disturbed the migration of cranial neural crest cells and abnormal craniofacial development, as a functionally relevant TAF11 variant affected cell migration via modulating CDH1 and CTNND1, which was associated with the etiology of non-syndromic cleft lip only [2].
In conclusion, Taf11 has important functions in processes like RLC assembly and cell migration. The study of Taf11 using genetic models such as Drosophila and zebrafish has provided insights into its role in RNAi efficiency and craniofacial development-related diseases, helping us better understand its biological significance and potential implications in disease mechanisms.
References：
1. Liang, Chunyang, Wang, Yibing, Murota, Yukiko, Siomi, Mikiko C, Liu, Qinghua. 2015. TAF11 Assembles the RISC Loading Complex to Enhance RNAi Efficiency. In Molecular cell, 59, 807-18. doi:10.1016/j.molcel.2015.07.006. https://pubmed.ncbi.nlm.nih.gov/26257286/
2. Li, Dandan, Tian, Yu, Vona, Barbara, Wang, Lin, Pan, Yongchu. . A TAF11 variant contributes to non-syndromic cleft lip only through modulating neural crest cell migration. In Human molecular genetics, 34, 392-401. doi:10.1093/hmg/ddae188. https://pubmed.ncbi.nlm.nih.gov/39727181/
3. Robinson, M M, Yatherajam, G, Ranallo, R T, Paule, M R, Stargell, L A. . Mapping and functional characterization of the TAF11 interaction with TFIIA. In Molecular and cellular biology, 25, 945-57. doi:. https://pubmed.ncbi.nlm.nih.gov/15657423/
4. Gupta, Kapil, Watson, Aleksandra A, Baptista, Tiago, Tora, Làszlò, Berger, Imre. 2017. Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID. In eLife, 6, . doi:10.7554/eLife.30395. https://pubmed.ncbi.nlm.nih.gov/29111974/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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