C57BL/6JCya-Fam162aem1flox/Cya
Common Name:
Fam162a-flox
Product ID:
S-CKO-14749
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fam162a-flox
Strain ID
CKOCMP-70186-Fam162a-B6J-VA
Gene Name
Product ID
S-CKO-14749
Gene Alias
2310056P07Rik; HGTD-P
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fam162aem1flox/Cya mice (Catalog S-CKO-14749) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000004057
NCBI RefSeq
NM_027342
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Fam162a, with its specific function yet to be fully elucidated, has been associated with several biological processes. It has been linked to hypoxia-related pathways, glycolysis-related processes, and may play a role in apoptosis regulation. Its involvement in these pathways indicates its importance in maintaining cellular homeostasis and response to various stressors [2,3,4,5,6,7,8,9].
In the context of disease, in patients with inflammatory bowel disease (IBD), Fam162a was identified as one of the 10 marker genes potentially contributing to intestinal barrier repairing, with its expression specific to absorptive cell types in intestinal epithelium [1]. In coronary artery disease (CAD), it was among 4 hub signature genes identified as hypoxia-related genes, and was found to be highly expressed in endothelial cells [2]. In osteosarcoma, a gene signature including Fam162a was constructed related to hypoxia and lactate metabolism, with high-risk scores (where Fam162a was part of the signature) associated with poor prognosis and "cold" tumor characteristics [3]. In IDH-mutant glioma, a hypoxia-related survival score containing Fam162a was identified, which could predict survival independent of other prognostic factors [4]. In colon cancer, Fam162a was part of a 13-gene glycolysis-related prognostic prediction model [5]. In a study on the mechanism of neuronal apoptosis in chronic cerebral ischemia, Foxh1 was shown to transcriptionally promote the expression of Fam162a [6]. In tilapia, differential exon usages of fam162a were detected in response to acute hypoxia treatment in the heart [7]. In mouse neuroblastoma Neuro-2a cells exposed to a mitochondrial toxin, there was a loss of FAM162a mRNA along with changes in mitochondrial-related genes [8]. In osteosarcoma, a risk model based on seven glycolytic genes including Fam162a could effectively evaluate prognosis [9].
In conclusion, Fam162a is implicated in multiple biological processes and disease conditions, especially those related to hypoxia, glycolysis, and tissue repair or tumor prognosis. Studies across different disease models have revealed its potential as a biomarker or therapeutic target in diseases such as IBD, CAD, osteosarcoma, IDH-mutant glioma, and colon cancer. Understanding the function of Fam162a through these disease-based research models provides insights into the underlying molecular mechanisms of these diseases.
References:
1. Zhao, Xiao-Hu, Zhao, Peinan, Deng, Zihao, Sun, Da-Li, He, Hai-Yu. 2023. Integrative analysis reveals marker genes for intestinal mucosa barrier repairing in clinical patients. In iScience, 26, 106831. doi:10.1016/j.isci.2023.106831. https://pubmed.ncbi.nlm.nih.gov/37250791/
2. Jin, Yuqing, Ren, Weiyan, Liu, Jiayi, Hou, Lianguo, Yang, Lei. 2023. Identification and validation of potential hypoxia-related genes associated with coronary artery disease. In Frontiers in physiology, 14, 1181510. doi:10.3389/fphys.2023.1181510. https://pubmed.ncbi.nlm.nih.gov/37637145/
3. Wang, Yizhuo, Wang, Xin, Liu, Yang, Zheng, Yufu, Qi, Quan. 2024. A novel hypoxia- and lactate metabolism-related prognostic signature to characterize the immune landscape and predict immunotherapy response in osteosarcoma. In Frontiers in immunology, 15, 1467052. doi:10.3389/fimmu.2024.1467052. https://pubmed.ncbi.nlm.nih.gov/39569192/
4. Dao Trong, Philip, Rösch, Saskia, Mairbäurl, Heimo, Herold-Mende, Christel, Warta, Rolf. 2018. Identification of a Prognostic Hypoxia-Associated Gene Set in IDH-Mutant Glioma. In International journal of molecular sciences, 19, . doi:10.3390/ijms19102903. https://pubmed.ncbi.nlm.nih.gov/30257451/
5. Liu, Gang, Wu, Xiaoyang, Chen, Jian. 2022. Identification and validation of a glycolysis-related gene signature for depicting clinical characteristics and its relationship with tumor immunity in patients with colon cancer. In Aging, 14, 8700-8718. doi:10.18632/aging.204226. https://pubmed.ncbi.nlm.nih.gov/35963622/
6. Yang, Jin, Liu, Xiaobai, Zhao, Yubo, Cui, Zheng, Liu, Yunhui. 2023. Mechanism of Dcp2/RNCR3/Dkc1/Snora62 axis regulating neuronal apoptosis in chronic cerebral ischemia. In Cell biology and toxicology, 39, 2881-2898. doi:10.1007/s10565-023-09807-8. https://pubmed.ncbi.nlm.nih.gov/37097350/
7. Xia, Jun Hong, Li, Hong Lian, Li, Bi Jun, Gu, Xiao Hui, Lin, Hao Ran. 2017. Acute hypoxia stress induced abundant differential expression genes and alternative splicing events in heart of tilapia. In Gene, 639, 52-61. doi:10.1016/j.gene.2017.10.002. https://pubmed.ncbi.nlm.nih.gov/28986317/
8. Mazzio, E, Soliman, K F A. 2012. Whole genome expression profile in neuroblastoma cells exposed to 1-methyl-4-phenylpyridine. In Neurotoxicology, 33, 1156-69. doi:10.1016/j.neuro.2012.06.009. https://pubmed.ncbi.nlm.nih.gov/22776087/
9. Huang, Wei, Xiao, Yingqi, Wang, Hongwei, Chen, Guanghui, Li, Kaixiang. 2022. Identification of risk model based on glycolysis-related genes in the metastasis of osteosarcoma. In Frontiers in endocrinology, 13, 1047433. doi:10.3389/fendo.2022.1047433. https://pubmed.ncbi.nlm.nih.gov/36387908/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen