C57BL/6JCya-Nipal1em1flox/Cya
Common Name:
Nipal1-flox
Product ID:
S-CKO-14898
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nipal1-flox
Strain ID
CKOCMP-70701-Nipal1-B6J-VA
Gene Name
Product ID
S-CKO-14898
Gene Alias
3830408G10Rik; Npal1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nipal1em1flox/Cya mice (Catalog S-CKO-14898) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087212
NCBI RefSeq
NM_001081205
Target Region
Exon 4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
NIPAL1, encoding a magnesium influx transporter, is a gene with significant biological importance. It is involved in magnesium-related cellular processes and has been associated with multiple biological pathways and diseases [1,2,5]. Genetic models can be valuable in studying its functions.
In pancreatic islets, NIPAL1 expression is regulated by extracellular magnesium. Knockdown of NIPAL1 in Min6-K8 cells (a pancreatic β-cell-like cell line) decreased both basal insulin secretion and total insulin content, while its overexpression increased total insulin content, suggesting its role in glucose-stimulated insulin secretion and intracellular insulin content regulation, especially under hypomagnesemia conditions [1].
In oral squamous cell carcinoma (OSCC), NIPAL1 expression was observed in 20.3% of 192 patients. Its expression correlated with cancer cell intravasation and poorer disease-free survival, and in functional analysis, it regulated the growth and adhesion of OSCC tumor cells and endothelial cells, indicating it might be a novel tumor-promoting factor and a useful molecular marker for OSCC [2].
In a genome-wide association study, NIPAL1 achieved Bonferroni significance in gene-based association tests related to spondylosis [3]. Genome-wide association studies also linked NIPAL1 to gout associated with reduced renal urate excretion, though functional analysis did not detect urate transport via NIPAL1, and its expression in the human kidney was mainly in the distal tubules [4,5,6,7].
In conclusion, NIPAL1 plays essential roles in multiple biological processes and disease conditions. Its function in insulin secretion regulation in pancreatic islets, its potential as a tumor-promoting factor in OSCC, and its associations with spondylosis and gout are significant findings. Studies using various models, including potential KO/CKO mouse models (not explicitly detailed in provided references but inferred as valuable for further study), contribute to understanding these functions and their implications in related diseases.
References：
1. Manialawy, Yousef, Khan, Saifur R, Bhattacharjee, Alpana, Wheeler, Michael B. 2020. The magnesium transporter NIPAL1 is a pancreatic islet-expressed protein that conditionally impacts insulin secretion. In The Journal of biological chemistry, 295, 9879-9892. doi:10.1074/jbc.RA120.013277. https://pubmed.ncbi.nlm.nih.gov/32439805/
2. Sasahira, Tomonori, Nishiguchi, Yukiko, Kurihara-Shimomura, Miyako, Kuniyasu, Hiroki, Kirita, Tadaaki. 2018. NIPA-like domain containing 1 is a novel tumor-promoting factor in oral squamous cell carcinoma. In Journal of cancer research and clinical oncology, 144, 875-882. doi:10.1007/s00432-018-2612-x. https://pubmed.ncbi.nlm.nih.gov/29464350/
3. Zhang, Yanfei, Grant, Ryan A, Shivakumar, Manu K, Williams, Marc S, Lee, Ming Ta Michael. . Genome-wide Association Analysis Across 16,956 Patients Identifies a Novel Genetic Association Between BMP6, NIPAL1, CNGA1 and Spondylosis. In Spine, 46, E625-E631. doi:10.1097/BRS.0000000000003880. https://pubmed.ncbi.nlm.nih.gov/33332786/
4. García-Nieto, Víctor M, Claverie-Martín, Félix, Moraleda-Mesa, Teresa, Luis-Yanes, María I, Ramos-Trujillo, Elena. 2022. Gout associated with reduced renal excretion of uric acid. Renal tubular disorder that nephrologists do not treat. In Nefrologia, 42, 273-279. doi:10.1016/j.nefroe.2022.05.007. https://pubmed.ncbi.nlm.nih.gov/36210617/
5. Nakayama, Akiyoshi, Nakaoka, Hirofumi, Yamamoto, Ken, Merriman, Tony R, Matsuo, Hirotaka. 2016. GWAS of clinically defined gout and subtypes identifies multiple susceptibility loci that include urate transporter genes. In Annals of the rheumatic diseases, 76, 869-877. doi:10.1136/annrheumdis-2016-209632. https://pubmed.ncbi.nlm.nih.gov/27899376/
6. García-Nieto, Víctor M, Claverie-Martín, Félix, Moraleda-Mesa, Teresa, Luis-Yanes, María I, Ramos-Trujillo, Elena. 2021. Gout associated with reduced renal excretion of uric acid. Renal tubular disorder that nephrologists do not treat. In Nefrologia, , . doi:10.1016/j.nefro.2021.03.013. https://pubmed.ncbi.nlm.nih.gov/34503865/
7. Zhu, Weifeng, Deng, Yan, Zhou, Xiaodong. 2018. Multiple Membrane Transporters and Some Immune Regulatory Genes are Major Genetic Factors to Gout. In The open rheumatology journal, 12, 94-113. doi:10.2174/1874312901812010094. https://pubmed.ncbi.nlm.nih.gov/30123371/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen