C57BL/6JCya-Mettl13em1flox/Cya
Common Name:
Mettl13-flox
Product ID:
S-CKO-15077
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mettl13-flox
Strain ID
CKOCMP-71449-Mettl13-B6J-VA
Gene Name
Product ID
S-CKO-15077
Gene Alias
5630401D24Rik; Eef1aknmt
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mettl13em1flox/Cya mice (Catalog S-CKO-15077) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028017
NCBI RefSeq
NM_144877
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
METTL13, also known as eEF1A-KNMT and FEAT, is a dual methyltransferase. It targets the N-terminus and Lys55 in eukaryotic translation elongation factor 1 alpha (eEF1A). METTL13-mediated methylation of eEF1A impacts translation dynamics, including the rate of global protein synthesis and translation of specific codons, and is associated with multiple biological processes and diseases, especially cancers [5].
In Ras-driven cancers, METTL13 deletion and eEF1AK55me2 loss significantly reduce neoplastic growth in mouse models and patient-derived xenografts (PDXs) from primary pancreatic and lung tumors, indicating its role in promoting tumorigenesis through increasing translational output [1]. In nasopharyngeal carcinoma (NPC) cells, METTL13 silencing suppresses cellular phenotypes like proliferation, migration, invasion, and angiogenesis in vitro and hinders tumorigenesis in vivo [2]. In gastric cancer, silencing METTL13 suppresses cell proliferation and metastasis in vivo and vitro [4]. In clear cell renal cell carcinoma (ccRCC), knockdown and overexpression of METTL13 respectively lead to an increase and decrease in ccRCC cells' proliferation, viability, migratory and invasive abilities, as well as epithelial-mesenchymal transition (EMT) in vitro, and METTL13 inhibits ccRCC cells' growth and metastasis in vivo [6]. In bladder carcinoma, although the original study was retracted, it was initially reported that METTL13 negatively regulated cell proliferation, migration, and invasion [7,8]. In the heart, cardiomyocyte-specific overexpression of Mettl13 attenuated cardiac contractile dysfunction and fibrosis in response to myocardial infarction in mice, while silencing impaired cardiac function [3].
In conclusion, METTL13 plays a crucial role in multiple biological processes, especially in cancer and cardiac function. Studies using gene knockout or knockdown models in mice and in vitro cell models have revealed its oncogenic or tumor-suppressive roles in different cancers and its protective role in ischemic heart failure, providing potential therapeutic targets for these diseases.
References:
1. Liu, Shuo, Hausmann, Simone, Carlson, Scott Moore, Mazur, Pawel Karol, Gozani, Or. 2019. METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis. In Cell, 176, 491-504.e21. doi:10.1016/j.cell.2018.11.038. https://pubmed.ncbi.nlm.nih.gov/30612740/
2. Ni, Haifeng, Liang, Chengxian, Zhou, Zhen, Shang, Haiqiong, Yu, Xiaoyu. 2023. METTL13 promotes nasopharyngeal carcinoma progression through regulating the ZEB1/TPT1 axis. In The journal of gene medicine, 25, e3476. doi:10.1002/jgm.3476. https://pubmed.ncbi.nlm.nih.gov/36735630/
3. Yu, Shuting, Sun, ZhiYong, Wang, Xiuzhu, Yang, Baofeng, Du, Weijie. 2023. Mettl13 protects against cardiac contractile dysfunction by negatively regulating C-Cbl-mediated ubiquitination of SERCA2a in ischemic heart failure. In Science China. Life sciences, 66, 2786-2804. doi:10.1007/s11427-022-2351-1. https://pubmed.ncbi.nlm.nih.gov/37450238/
4. Wu, Qiong, Hu, Qingqing, Hai, Yanan, Li, Yandong, Gao, Yong. 2022. METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit. In Journal of cell communication and signaling, 17, 121-135. doi:10.1007/s12079-022-00687-x. https://pubmed.ncbi.nlm.nih.gov/35925508/
5. Jakobsson, Magnus E. 2021. Structure, Activity and Function of the Dual Protein Lysine and Protein N-Terminal Methyltransferase METTL13. In Life (Basel, Switzerland), 11, . doi:10.3390/life11111121. https://pubmed.ncbi.nlm.nih.gov/34832997/
6. Liu, Zhuonan, Sun, Tianshui, Piao, Chiyuan, Zhang, Zhe, Kong, Chuize. 2021. METTL13 inhibits progression of clear cell renal cell carcinoma with repression on PI3K/AKT/mTOR/HIF-1α pathway and c-Myc expression. In Journal of translational medicine, 19, 209. doi:10.1186/s12967-021-02879-2. https://pubmed.ncbi.nlm.nih.gov/33985542/
7. Zhang, Zhe, Zhang, Guojun, Kong, Chuize, Dong, Xiao, Man, Xiaojun. 2016. METTL13 is downregulated in bladder carcinoma and suppresses cell proliferation, migration and invasion. In Scientific reports, 6, 19261. doi:10.1038/srep19261. https://pubmed.ncbi.nlm.nih.gov/26763933/
8. Zhang, Zhe, Zhang, Guojun, Kong, Chuize, Dong, Xiao, Man, Xiaojun. 2023. Retraction Note: METTL13 is downregulated in bladder carcinoma and suppresses cell proliferation, migration and invasion. In Scientific reports, 13, 11916. doi:10.1038/s41598-023-39010-y. https://pubmed.ncbi.nlm.nih.gov/37488192/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen