C57BL/6JCya-Rbpms2em1flox/Cya
Common Name:
Rbpms2-flox
Product ID:
S-CKO-15290
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rbpms2-flox
Strain ID
CKOCMP-71973-Rbpms2-B6J-VA
Gene Name
Product ID
S-CKO-15290
Gene Alias
2400008B06Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbpms2em1flox/Cya mice (Catalog S-CKO-15290) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055844
NCBI RefSeq
NM_028030
Target Region
Exon 2~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Rbpms2, an RNA-binding protein, is a member of the RNA-recognition-motif-containing protein family. It is involved in multiple biological processes such as alternative splicing regulation, sexual differentiation, and early embryo development. In the heart, it is a myocardial-enriched splicing regulator crucial for cardiac function. In sexual development, it promotes female fate and is related to pathways like mTorc1 signaling. Genetic models like knockout zebrafish and mice are valuable for studying its functions [1,2,3,5,6].
In zebrafish, rbpms2-deficient embryos have early cardiac dysfunction, myofibril disarray, and altered calcium handling, along with differential alternative splicing events. These phenotypes are also seen in RBPMS2-deficient human cardiomyocytes, uncovering a conserved network of 29 ortholog pairs regulated by RBPMS2 for alternative splicing [1]. In zebrafish gonads, rbpms2 mutants initially have early oocytes but definitive oogenesis fails during sexual differentiation, developing as fertile males. Rbpms2 promotes nucleolar amplification via the mTorc1 signaling pathway through the Gator2 component Mios [2,3,4]. In mouse models, Rbpms and Rbpms2 double cardiomyocyte-specific knockout mice die before embryonic day 13.5 with sarcomere disarray due to widespread mis-splicing of genes essential for cardiac contraction, while single knockout mice survive to adulthood [5].
In summary, Rbpms2 is essential for cardiac function, oogenesis, and sexual differentiation. Studies using gene knockout models in zebrafish and mice have revealed its role in regulating alternative splicing in the heart, and its function in promoting female fate in gonads. These findings contribute to understanding cardiovascular diseases and reproductive disorders [1,2,3,4,5].
References:
1. Akerberg, Alexander A, Trembley, Michael, Butty, Vincent, Burns, Caroline E, Burns, C Geoffrey. 2022. RBPMS2 Is a Myocardial-Enriched Splicing Regulator Required for Cardiac Function. In Circulation research, 131, 980-1000. doi:10.1161/CIRCRESAHA.122.321728. https://pubmed.ncbi.nlm.nih.gov/36367103/
2. Wilson, Miranda L, Romano, Shannon N, Khatri, Nitya, Draper, Bruce W, Marlow, Florence L. 2024. Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios. In Nature communications, 15, 5248. doi:10.1038/s41467-024-49613-2. https://pubmed.ncbi.nlm.nih.gov/38898112/
3. Kaufman, Odelya H, Lee, KathyAnn, Martin, Manon, Rothhämel, Sophie, Marlow, Florence L. 2018. rbpms2 functions in Balbiani body architecture and ovary fate. In PLoS genetics, 14, e1007489. doi:10.1371/journal.pgen.1007489. https://pubmed.ncbi.nlm.nih.gov/29975683/
4. Wilson, Miranda L, Romano, Shannon N, Khatri, Nitya, Draper, Bruce W, Marlow, Florence L. 2024. Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component, Mios. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.25.577235. https://pubmed.ncbi.nlm.nih.gov/38328218/
5. Wu, Tongbin, Chen, Zeyu, Zhang, Zengming, Dinenno, Frank A, Chen, Ju. 2024. RBPMS and RBPMS2 Cooperate to Safeguard Cardiac Splicing. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.11.07.622565. https://pubmed.ncbi.nlm.nih.gov/39574760/
6. Zhou, Cheng, Zheng, Lu, Teng, Hui, Chen, Li, Yao, Bing. 2023. Maternal RNA binding protein with multiple splicing 2 (RBPMS2) is involved in mouse blastocyst formation through the bone morphogenetic protein pathway. In Reproductive biomedicine online, 47, 103238. doi:10.1016/j.rbmo.2023.05.010. https://pubmed.ncbi.nlm.nih.gov/37573751/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen