C57BL/6JCya-Tmem163em1flox/Cya
Common Name:
Tmem163-flox
Product ID:
S-CKO-15381
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem163-flox
Strain ID
CKOCMP-72160-Tmem163-B6J-VA
Gene Name
Product ID
S-CKO-15381
Gene Alias
2610024A01Rik; A-cre; Act-Cre; SV31; Tg(ACTB-cre)2Mrt; actin-cre; cre<actin>
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem163em1flox/Cya mice (Catalog S-CKO-15381) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000185560
NCBI RefSeq
NM_028135
Target Region
Exon 3~4
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
TMEM163, a zinc efflux transporter, is likely a new member of the mammalian SLC30 (ZnT) subfamily of the cation diffusion facilitator (CDF) protein superfamily. It plays a role in zinc metabolism and is involved in multiple biological processes. It has been associated with various human diseases, indicating its overall biological importance [1,2,3,4].
Functional zinc flux assays in HeLa cells expressing TMEM163 protein variants (L76R and L76P) showed distinct attenuation or enhancement of zinc efflux respectively. In zebrafish models with knockdown of tmem163a and tmem163b, loss of tmem163 caused dysplasia of the larvae, locomotor disability, and myelin deficit. Expression of human wild-type TMEM163 mRNAs rescued the phenotype, while the mutants aggravated the condition. Also, in an oligodendroglial cell line, mutant TMEM163 proteins led to reduced mRNA expression of key myelin genes, reduced branching, and increased cell death. These findings suggest its role in myelin development [3,4].
In conclusion, TMEM163 is crucial for zinc efflux and is involved in myelin development. Studies on zebrafish models with tmem163 knockdown have revealed its significance in myelin-related processes, adding TMEM163 to the list of genes associated with hypomyelination leukodystrophy.
References：
1. Styrpejko, Daniel J, Cuajungco, Math P. 2021. Transmembrane 163 (TMEM163) Protein: A New Member of the Zinc Efflux Transporter Family. In Biomedicines, 9, . doi:10.3390/biomedicines9020220. https://pubmed.ncbi.nlm.nih.gov/33670071/
2. Sanchez, Vanessa B, Ali, Saima, Escobar, Adrian, Cuajungco, Math P. 2019. Transmembrane 163 (TMEM163) protein effluxes zinc. In Archives of biochemistry and biophysics, 677, 108166. doi:10.1016/j.abb.2019.108166. https://pubmed.ncbi.nlm.nih.gov/31697912/
3. Yan, Huifang, Yang, Shuyan, Hou, Yiming, Cuajungco, Math P, Wang, Jingmin. 2022. Functional Study of TMEM163 Gene Variants Associated with Hypomyelination Leukodystrophy. In Cells, 11, . doi:10.3390/cells11081285. https://pubmed.ncbi.nlm.nih.gov/35455965/
4. do Rosario, Michelle C, Bey, Guillermo Rodriguez, Nmezi, Bruce, Padiath, Quasar Saleem, Shukla, Anju. . Variants in the zinc transporter TMEM163 cause a hypomyelinating leukodystrophy. In Brain : a journal of neurology, 145, 4202-4209. doi:10.1093/brain/awac295. https://pubmed.ncbi.nlm.nih.gov/35953447/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen