C57BL/6JCya-Slc16a10em1flox/Cya
Common Name:
Slc16a10-flox
Product ID:
S-CKO-15481
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc16a10-flox
Strain ID
CKOCMP-72472-Slc16a10-B6J-VA
Gene Name
Product ID
S-CKO-15481
Gene Alias
2610103N14Rik; 9830169E08; Mct10; PRO0813; TAT1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc16a10em1flox/Cya mice (Catalog S-CKO-15481) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000092566
NCBI RefSeq
NM_001114332
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Slc16a10, also known as TAT1 (T-type amino acid transporter 1), is an aromatic amino acid transporter. It is involved in the facilitated diffusion of aromatic amino acids across membranes, such as basolateral membranes of kidney, small intestine and liver epithelial cells, and plasma membranes of non-epithelial cells like skeletal myocytes [2,5,6]. This function is crucial for maintaining extracellular aromatic amino acid homeostasis, and it may also play a role in the efflux of other neutral amino acids [5,6]. It is also associated with processes like melanogenesis and tryptophan homeostasis [1,3].
In Slc16a10-defective mice (tat1(-/-)), plasma, muscle and kidney aromatic amino acid (AAA) concentrations increase under normal and high-protein diets, indicating its role in equilibrating AAA concentrations across specific membranes [5]. In melanocytes, overexpression of Slc16a10 promotes melanogenesis by enhancing the uptake of phenylalanine, and knockdown reduces UVB-induced melanin production and phenylalanine uptake [1]. In A549 cells, knocking down Slc16a10 reduces the expression of inflammatory factors IL-1β and TNF-α after LPS treatment, suggesting its potential role in acute lung injury [4].
In conclusion, Slc16a10 is essential for maintaining aromatic amino acid homeostasis and has implications in processes like melanogenesis and the inflammatory response in acute lung injury. Studies using gene-knockout mouse models have been instrumental in revealing these functions, highlighting its significance in understanding physiological and pathological processes related to amino acid metabolism and associated diseases.
References:
1. Luo, Liping, Zeng, Hongliang, Hu, Yibo, Chen, Jing, Zeng, Qinghai. . The amino acid transporter SLC16A10 promotes melanogenesis by facilitating the transportation of phenylalanine. In Experimental dermatology, 33, e15165. doi:10.1111/exd.15165. https://pubmed.ncbi.nlm.nih.gov/39171634/
2. Halestrap, Andrew P. . The SLC16 gene family - structure, role and regulation in health and disease. In Molecular aspects of medicine, 34, 337-49. doi:10.1016/j.mam.2012.05.003. https://pubmed.ncbi.nlm.nih.gov/23506875/
3. Klaessens, Simon, Stroobant, Vincent, De Plaen, Etienne, Van den Eynde, Benoit J. 2022. Systemic tryptophan homeostasis. In Frontiers in molecular biosciences, 9, 897929. doi:10.3389/fmolb.2022.897929. https://pubmed.ncbi.nlm.nih.gov/36188218/
4. Zeng, Huanan, Zhou, Yuqing, Liu, Zhi, Liu, Wei. 2024. MiR-21-5p modulates LPS-induced acute injury in alveolar epithelial cells by targeting SLC16A10. In Scientific reports, 14, 11160. doi:10.1038/s41598-024-61777-x. https://pubmed.ncbi.nlm.nih.gov/38750066/
5. Mariotta, Luca, Ramadan, Tamara, Singer, Dustin, Camargo, Simone M R, Verrey, François. 2012. T-type amino acid transporter TAT1 (Slc16a10) is essential for extracellular aromatic amino acid homeostasis control. In The Journal of physiology, 590, 6413-24. doi:10.1113/jphysiol.2012.239574. https://pubmed.ncbi.nlm.nih.gov/23045339/
6. Ramadan, Tamara, Camargo, Simone M R, Summa, Vanessa, Pos, Klaas M, Verrey, Francois. . Basolateral aromatic amino acid transporter TAT1 (Slc16a10) functions as an efflux pathway. In Journal of cellular physiology, 206, 771-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16245314/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen