C57BL/6JCya-Rhobtb3em1flox/Cya
Common Name:
Rhobtb3-flox
Product ID:
S-CKO-15667
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rhobtb3-flox
Strain ID
CKOCMP-73296-Rhobtb3-B6J-VA
Gene Name
Product ID
S-CKO-15667
Gene Alias
1700040C17Rik; 2610033K01Rik; 4930503C18Rik; mKIAA0878
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rhobtb3em1flox/Cya mice (Catalog S-CKO-15667) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022078
NCBI RefSeq
NM_028493
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
RhoBTB3, an atypical Rho GTPase family member, is involved in multiple cellular functions. It binds and hydrolyzes ATP, participating in diverse pathways such as endosome to Golgi transport, autophagy regulation, and cell cycle control. Its functions are crucial for maintaining cellular homeostasis and are relevant to various disease processes [4,2,6].
In breast cancer, decreasing RhoBTB3 expression leads to reduced proliferative and invasive properties of cancer cells, potentially through repressing Col1a1 expression, suggesting its role in tumor progression [1]. In autophagy, RhoBTB3 deficiency induces autophagy, while overexpression inhibits it by mediating the ubiquitination and degradation of AMBRA1 [2]. In renal carcinomas, its deficiency significantly elevates the Warburg effect and accelerates xenograft growth, as it promotes proteasomal degradation of HIFα [3]. In non-M3 acute myeloid leukemia, high RhoBTB3 expression predicts favorable chemotherapy outcomes [5].
In conclusion, RhoBTB3 plays essential roles in multiple biological processes and disease conditions. Research using gene knockout models, like in mice, has revealed its significance in cancer progression, autophagy, and treatment response in leukemia. Understanding RhoBTB3's functions provides potential therapeutic targets for related diseases.
References:
1. Kim, Kyungho, Kim, Youn-Jae. 2022. RhoBTB3 Regulates Proliferation and Invasion of Breast Cancer Cells via Col1a1. In Molecules and cells, 45, 631-639. doi:10.14348/molcells.2022.2037. https://pubmed.ncbi.nlm.nih.gov/35698915/
2. Kim, Kyungho, Kim, Dong-Gun, Kim, Youn-Jae. 2024. RhoBTB3 Functions as a Novel Regulator of Autophagy by Suppressing AMBRA1 Stability. In Cells, 13, . doi:10.3390/cells13191659. https://pubmed.ncbi.nlm.nih.gov/39404422/
3. Zhang, Chen-Song, Liu, Qi, Li, Mengqi, Ye, Zhiyun, Lin, Sheng-Cai. 2015. RHOBTB3 promotes proteasomal degradation of HIFα through facilitating hydroxylation and suppresses the Warburg effect. In Cell research, 25, 1025-42. doi:10.1038/cr.2015.90. https://pubmed.ncbi.nlm.nih.gov/26215701/
4. Espinosa, Eric J, Calero, Monica, Sridevi, Khambhampaty, Pfeffer, Suzanne R. . RhoBTB3: a Rho GTPase-family ATPase required for endosome to Golgi transport. In Cell, 137, 938-48. doi:10.1016/j.cell.2009.03.043. https://pubmed.ncbi.nlm.nih.gov/19490898/
5. Yang, Shuang-Hui, Liu, Wei, Peng, Jie, Chen, Cong, Liu, En-Yi. 2021. High Expression of RhoBTB3 Predicts Favorable Chemothrapy Outcomes in non-M3 Acute Myeloid Leukemia. In Journal of Cancer, 12, 4229-4239. doi:10.7150/jca.50472. https://pubmed.ncbi.nlm.nih.gov/34093823/
6. Lu, Albert, Pfeffer, Suzanne R. 2013. Golgi-associated RhoBTB3 targets cyclin E for ubiquitylation and promotes cell cycle progression. In The Journal of cell biology, 203, 233-50. doi:10.1083/jcb.201305158. https://pubmed.ncbi.nlm.nih.gov/24145166/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen