C57BL/6JCya-Rptorem1flox/Cya
Common Name:
Rptor-flox
Product ID:
S-CKO-15978
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rptor-flox
Strain ID
CKOCMP-74370-Rptor-B6J-VA
Gene Name
Product ID
S-CKO-15978
Gene Alias
4932417H02Rik; Rap; Raptor; mKIAA1303
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rptorem1flox/Cya mice (Catalog S-CKO-15978) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026671
NCBI RefSeq
NM_028898
Target Region
Exon 6
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Rptor, also known as regulatory associated protein of MTOR complex 1, is a key component of the mTORC1 pathway. The mTORC1 pathway is central in promoting growth in response to insulin, energy levels, and amino acids, and is often deregulated in cancers. Rptor plays a role in various biological processes, with its function being studied through genetic models to understand its importance in normal and disease-related conditions [3,5].
In esophageal squamous cell carcinoma (ESCC), METTL1 or WDR4 knockdown leads to decreased expression of m7G-modified tRNAs, reducing the translation of a subset of oncogenic transcripts enriched in the RPTOR/ULK1/autophagy pathway. Mettl1 conditional knockout and knockin mice models demonstrate the essential function of METTL1 in promoting ESCC tumorigenesis, suggesting that Rptor in this axis may be a potential therapeutic target [1].
In NSCLC brain metastasis, RPTOR was significantly upregulated in BM foci, and RPTOR blockade in xenograft and zebrafish models suppressed BM by interfering with ceramide metabolism, indicating its role as a key driver gene [2].
In melanoma, RPTOR-mutation patients had prolonged overall survival and a higher objective response rate to immune checkpoint inhibitors compared to RPTOR-wildtype patients, suggesting RPTOR mutation could be a predictor of effective immunotherapy [4].
In conclusion, Rptor is crucial in the mTORC1 pathway and plays significant roles in multiple disease conditions. Studies using gene knockout or conditional knockout mouse models have revealed its importance in cancer-related processes such as tumorigenesis, metastasis, and response to immunotherapy, providing insights for potential therapeutic strategies in these diseases.
References:
1. Han, Hui, Yang, Chunlong, Ma, Jieyi, Choe, Junho, Lin, Shuibin. 2022. N7-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma tumorigenesis via the RPTOR/ULK1/autophagy axis. In Nature communications, 13, 1478. doi:10.1038/s41467-022-29125-7. https://pubmed.ncbi.nlm.nih.gov/35304469/
2. Lin, Ying, Wu, Yun, Zhang, Qiangzu, Chen, Yusheng, Li, Hongru. 2024. RPTOR blockade suppresses brain metastases of NSCLC by interfering the ceramide metabolism via hijacking YY1 binding. In Journal of experimental & clinical cancer research : CR, 43, 1. doi:10.1186/s13046-023-02874-z. https://pubmed.ncbi.nlm.nih.gov/38163890/
3. Foerster, Elisabeth G, Mukherjee, Tapas, Cabral-Fernandes, Liliane, Girardin, Stephen E, Philpott, Dana J. 2021. How autophagy controls the intestinal epithelial barrier. In Autophagy, 18, 86-103. doi:10.1080/15548627.2021.1909406. https://pubmed.ncbi.nlm.nih.gov/33906557/
4. Jiang, Yanfang, Hu, Xintong, Wang, Zhouyu, Chen, Dongsheng, Zhao, Pingwei. 2023. RPTOR mutation: a novel predictor of efficacious immunotherapy in melanoma. In Investigational new drugs, 42, 60-69. doi:10.1007/s10637-023-01413-z. https://pubmed.ncbi.nlm.nih.gov/38071684/
5. Sancak, Yasemin, Peterson, Timothy R, Shaul, Yoav D, Bar-Peled, Liron, Sabatini, David M. 2008. The Rag GTPases bind raptor and mediate amino acid signaling to mTORC1. In Science (New York, N.Y.), 320, 1496-501. doi:10.1126/science.1157535. https://pubmed.ncbi.nlm.nih.gov/18497260/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen