C57BL/6JCya-Ppp1r1cem1flox/Cya
Common Name:
Ppp1r1c-flox
Product ID:
S-CKO-16189
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ppp1r1c-flox
Strain ID
CKOCMP-75276-Ppp1r1c-B6J-VA
Gene Name
Product ID
S-CKO-16189
Gene Alias
4930565M23Rik; 4930579P15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1r1cem1flox/Cya mice (Catalog S-CKO-16189) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000090760
NCBI RefSeq
NM_172420
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Ppp1r1c, also known as thymocyte cyclic AMP-regulated phosphoprotein (TARPP), is a protein-coding gene. It has been associated with various biological processes. For instance, it may be involved in neural outgrowth as its expression was upregulated during the regrowth of afferent neurons to cochlear hair cells [4]. It might also play a role in the cell cycle regulation as its expression pattern was associated with negative cell cycle regulation and cell cycle arrest processes in human Wharton's Jelly mesenchymal stem cells treated with Moringa oleifera leaf extract [3].
In glioblastoma, miR-182 targets Ppp1r1c. High expression of Ppp1r1c was associated with decreased susceptibility to temozolomide (TMZ) therapy in glioblastoma patients. Loss of miR-182 led to increased expression of Ppp1r1c, which in part explained disease progression and resistance to TMZ therapy [1]. In Markhoz goats, Ppp1r1c was identified as a candidate gene associated with litter size, though its exact role in reproduction is yet to be clearly understood [2].
In summary, Ppp1r1c is involved in multiple biological processes such as neural outgrowth and cell cycle regulation. In disease conditions, its role in glioblastoma resistance to TMZ therapy and its potential association with litter size in goats highlight its significance. Further functional studies, perhaps through gene knockout or conditional knockout mouse models in the future, could provide more insights into its exact mechanisms in these and other biological processes and disease areas.
References:
1. Liu, Liqiang, Zhang, Xiaowei, Nan, Chengrui, Hu, Hongchao, Liang, Zhaohui. 2017. MicroRNA-182 targets protein phosphatase 1 regulatory inhibitor subunit 1C in glioblastoma. In Oncotarget, 8, 114677-114684. doi:10.18632/oncotarget.21309. https://pubmed.ncbi.nlm.nih.gov/29383111/
2. Mahmoudi, Peyman, Rashidi, Amir, Nazari-Ghadikolaei, Anahit, Razmkabir, Mohammad, Huson, Heather Jay. 2022. Genome-wide association study reveals novel candidate genes for litter size in Markhoz goats. In Frontiers in veterinary science, 9, 1045589. doi:10.3389/fvets.2022.1045589. https://pubmed.ncbi.nlm.nih.gov/36504837/
3. Ramarao, Kivaandra Dayaa Rao, Somasundram, Chandran, Razali, Zuliana, Musa, Sabri, Achari, Vijayan Manickam. 2022. Antiproliferative effects of dried Moringa oleifera leaf extract on human Wharton's Jelly mesenchymal stem cells. In PloS one, 17, e0274814. doi:10.1371/journal.pone.0274814. https://pubmed.ncbi.nlm.nih.gov/36197921/
4. Wu, Chen-Chi, Brugeaud, Aurore, Seist, Richard, Edge, Albert S B, Stankovic, Konstantina M. 2020. Altered expression of genes regulating inflammation and synaptogenesis during regrowth of afferent neurons to cochlear hair cells. In PloS one, 15, e0238578. doi:10.1371/journal.pone.0238578. https://pubmed.ncbi.nlm.nih.gov/33001981/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen