C57BL/6JCya-Prkd3em1flox/Cya
Common Name
Prkd3-flox
Product ID
S-CKO-16193
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-75292-Prkd3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Prkd3-flox Mouse (Catalog S-CKO-16193) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Prkd3-flox
Strain ID
CKOCMP-75292-Prkd3-B6J-VA
Gene Name
Product ID
S-CKO-16193
Gene Alias
PKD3, Pkcnu, Prkcn, 4930557O20Rik, 5730497N19Rik
Background
C57BL/6JCya
Gene Full Name
protein kinase D3
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 17 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000119284
NCBI Transcript ID
NM_001171004
Target Region
Exon 6~7
Size of Effective Region
~1.3 kb
Overview of Gene Research
Prkd3, encoding protein kinase D3, is a serine/threonine protein kinase belonging to the protein kinase D family. It can be activated by stimuli like phorbol esters and G-protein-coupled receptor agonists, and is involved in promoting cell proliferation, growth, migration, and invasion in multiple cancer types [3].
In cancer research, PRKD3 knockdown in OSCC cells attenuated their migration and invasiveness, and high PRKD3 expression was associated with distant metastasis and poor prognosis [1]. In HCC, PRKD3 knockdown reduced cell proliferation and induced cell cycle arrest at G2/M phase [2]. In GC, PRKD3 knockdown impaired cell malignancy with a decline in proliferation, migration, and invasion, along with G2/M phase arrest [4]. In invasive breast cancer, depletion of PRKD3 led to alternation of cell cycle and decrease of cell migration ability [5]. In addition, deletion of Prkd3 in mice promoted liver fibrosis through profibrotic macrophage activation [6].
In conclusion, Prkd3 plays a significant role in promoting the malignant progression of various cancers and in liver fibrosis development. The use of Prkd3 knockout or knockdown models in these studies has been crucial in revealing its oncogenic functions in specific disease conditions, providing potential therapeutic targets for cancer treatment and insights into the mechanism of liver fibrosis.
References：
1. Chen, Z, Huang, Q, Xu, W, Yang, J, Zhang, L-J. . PRKD3 promotes malignant progression of OSCC by downregulating KLF16 expression. In European review for medical and pharmacological sciences, 24, 12709-12716. doi:10.26355/eurrev_202012_24169. https://pubmed.ncbi.nlm.nih.gov/33378018/
2. Tian, Ye, Xie, Bei, Wang, Shuaiyang, Ma, Lei, Li, Linjing. 2024. PRKD3 promotes proliferation of liver cancer cells: a downstream proteomics profiling study. In American journal of translational research, 16, 6384-6398. doi:10.62347/YLJE5332. https://pubmed.ncbi.nlm.nih.gov/39678603/
3. Liu, Yan, Song, Hang, Zhou, Yehui, Yu, Zhenghong, Chen, Liming. 2021. The oncogenic role of protein kinase D3 in cancer. In Journal of Cancer, 12, 735-739. doi:10.7150/jca.50899. https://pubmed.ncbi.nlm.nih.gov/33403031/
4. Wang, Shuaiyang, Xie, Bei, Deng, Haohua, Li, Jing, Li, Linjing. 2025. Effect of PRKD3 on cell cycle in gastric cancer progression and downstream regulatory networks. In Medical oncology (Northwood, London, England), 42, 135. doi:10.1007/s12032-025-02663-y. https://pubmed.ncbi.nlm.nih.gov/40131654/
5. Liu, Yan, Li, Jian, Zhang, Jun, Gu, Jun, Chen, Liming. 2017. Oncogenic Protein Kinase D3 Regulating Networks in Invasive Breast Cancer. In International journal of biological sciences, 13, 748-758. doi:10.7150/ijbs.18472. https://pubmed.ncbi.nlm.nih.gov/28656000/
6. Zhang, Shuya, Liu, Huan, Yin, Meimei, Yamasaki, Sho, Jin, Zheng Gen. 2020. Deletion of Protein Kinase D3 Promotes Liver Fibrosis in Mice. In Hepatology (Baltimore, Md.), 72, 1717-1734. doi:10.1002/hep.31176. https://pubmed.ncbi.nlm.nih.gov/32048304/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
