C57BL/6NCya-Mettl5em1flox/Cya
Common Name:
Mettl5-flox
Product ID:
S-CKO-16216
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mettl5-flox
Strain ID
CKOCMP-75422-Mettl5-B6N-VA
Gene Name
Product ID
S-CKO-16216
Gene Alias
2810410A08Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mettl5em1flox/Cya mice (Catalog S-CKO-16216) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060447
NCBI RefSeq
NM_029280
Target Region
Exon 2~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
METTL5, an N6-adenosine methyltransferase, is crucial for 18S rRNA m6A modification. This modification is involved in multiple biological processes, such as ribosome synthesis, mRNA translation, and cell metabolism regulation. It also plays a role in various pathways including those related to cancer development and craniofacial development [3,4].
In cancer research, METTL5 has been found to be a key player. In hepatocellular carcinoma (HCC), METTL5 promotes glucose metabolic reprogramming, cell proliferation, and metastasis by stabilizing c-Myc through facilitating USP5 translation [1]. In intrahepatic cholangiocarcinoma (ICC), METTL5-mediated 18S rRNA m6A modification promotes oncogenic mRNA translation and tumor progression [2]. In gastric cancer, METTL5 promotes tumor development via sphingomyelin metabolism and confers cisplatin resistance [5]. In nonsmall cell lung cancer (NSCLC), METTL5 promotes cancer cell proliferation by interacting with IGF2BP3 [7]. In addition, in a mouse model, deletion of Mettl5 leads to poor ossification, widened cranial sutures, and a cleidocranial dysplasia-like phenotype, with Wnt signaling significantly downregulated, indicating its role in craniofacial development [6].
In conclusion, METTL5 is essential for 18S rRNA m6A modification, which impacts multiple biological processes. Its dysregulation is closely associated with various cancers and craniofacial development disorders. Gene knockout and conditional knockout mouse models have been instrumental in revealing these functions, providing potential therapeutic targets for related diseases.
References：
1. Xia, Peng, Zhang, Hao, Lu, Haofeng, Zhang, Zhonglin, Yuan, Yufeng. 2023. METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression. In Cancer communications (London, England), 43, 338-364. doi:10.1002/cac2.12403. https://pubmed.ncbi.nlm.nih.gov/36602428/
2. Dai, Zihao, Zhu, Wanjie, Hou, Yingdong, Lin, Shuibin, Kuang, Ming. 2023. METTL5-mediated 18S rRNA m6A modification promotes oncogenic mRNA translation and intrahepatic cholangiocarcinoma progression. In Molecular therapy : the journal of the American Society of Gene Therapy, 31, 3225-3242. doi:10.1016/j.ymthe.2023.09.014. https://pubmed.ncbi.nlm.nih.gov/37735874/
3. van Tran, Nhan, Ernst, Felix G M, Hawley, Ben R, Graille, Marc, Lafontaine, Denis L J. . The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112. In Nucleic acids research, 47, 7719-7733. doi:10.1093/nar/gkz619. https://pubmed.ncbi.nlm.nih.gov/31328227/
4. Turkalj, Elena M, Vissers, Caroline. 2022. The emerging importance of METTL5-mediated ribosomal RNA methylation. In Experimental & molecular medicine, 54, 1617-1625. doi:10.1038/s12276-022-00869-y. https://pubmed.ncbi.nlm.nih.gov/36266443/
5. Zhang, Ya-Qiong, Li, Jian, Qin, Zhe, Zhang, Xiao-Hong, Feng, Li. . METTL5 promotes gastric cancer progression via sphingomyelin metabolism. In World journal of gastrointestinal oncology, 16, 1925-1946. doi:10.4251/wjgo.v16.i5.1925. https://pubmed.ncbi.nlm.nih.gov/38764837/
6. Lei, Kexin, Xu, Ruoshi, Wang, Qian, Zhou, Chenchen, Yuan, Quan. 2022. METTL5 regulates cranial suture fusion via Wnt signaling. In Fundamental research, 3, 369-376. doi:10.1016/j.fmre.2022.04.005. https://pubmed.ncbi.nlm.nih.gov/38933773/
7. Gong, Sihan, Liu, Hu, Gou, Hao, Sun, Wanli. 2024. METTL5: A Potential Biomarker for Nonsmall Cell Lung Cancer That Promotes Cancer Cell Proliferation by Interacting with IGF2BP3. In Genetic testing and molecular biomarkers, 28, 311-321. doi:10.1089/gtmb.2023.0531. https://pubmed.ncbi.nlm.nih.gov/39023781/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen