C57BL/6JCya-Brd8em1flox/Cya
Common Name:
Brd8-flox
Product ID:
S-CKO-16839
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Brd8-flox
Strain ID
CKOCMP-78656-Brd8-B6J-VA
Gene Name
Product ID
S-CKO-16839
Gene Alias
2610007E11Rik; 4432404P07Rik; SMAP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Brd8em1flox/Cya mice (Catalog S-CKO-16839) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003876
NCBI RefSeq
NM_030147
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Brd8, also known as skeletal muscle abundant protein and thyroid hormone receptor coactivating protein of 120 kDa (TrCP120), is a subunit of the NuA4/TIP60-histone acetyltransferase complex. It functions in transcriptional modulation by recognizing acetylated lysine residues on histones and other proteins, and is involved in various biological processes such as cell proliferation, cell cycle progression, and response to cytotoxic agents [2,4]. It is also associated with several diseases, especially cancers [2-4, 10].
In glioblastoma (GBM), BRD8 maintains H2AZ occupancy at p53 target loci through the EP400 histone acetyltransferase complex, leading to a repressive chromatin state that prevents p53 transactivation and sustains proliferation. Targeting BRD8's bromodomain can displace H2AZ, enhance chromatin accessibility, and enforce cell cycle arrest and tumour suppression in TP53 wild-type GBM [1]. In hepatocellular carcinoma (HCC), BRD8, negatively regulated by miR-876-3p, promotes cell proliferation and apoptosis resistance via KAT5 [3]. In colorectal cancer cells, depletion of BRD8 induces cell-cycle arrest at the G1 phase and suppresses cell proliferation by regulating the expression of multiple subunits of the pre-replicative complex [4]. In human lung epithelial cells, depletion of BRD8 increases the secretion of antimicrobial peptide beta-defensin 1 and multiple chemokines, and reduces cell proliferation [5].
In conclusion, Brd8 plays a crucial role in cell-cycle regulation, cell proliferation, and apoptosis in various cancer types. Studies on Brd8 knockout or conditional knockout models have revealed its significance in specific disease areas like glioblastoma, hepatocellular carcinoma, and colorectal cancer. These findings provide potential therapeutic strategies for targeting Brd8 in cancer treatment.
References:
1. Sun, Xueqin, Klingbeil, Olaf, Lu, Bin, Vakoc, Christopher R, Mills, Alea A. 2022. BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network. In Nature, 613, 195-202. doi:10.1038/s41586-022-05551-x. https://pubmed.ncbi.nlm.nih.gov/36544023/
2. Yamaguchi, Kiyoshi, Nakagawa, Saya, Furukawa, Yoichi. 2024. Understanding the role of BRD8 in human carcinogenesis. In Cancer science, 115, 2862-2870. doi:10.1111/cas.16263. https://pubmed.ncbi.nlm.nih.gov/38965933/
3. Yu, Zhaoxiang, Chen, Tianxiang, Mo, Huanye, Guo, Cheng, Liu, Qingguang. 2020. BRD8, which is negatively regulated by miR-876-3p, promotes the proliferation and apoptosis resistance of hepatocellular carcinoma cells via KAT5. In Archives of biochemistry and biophysics, 693, 108550. doi:10.1016/j.abb.2020.108550. https://pubmed.ncbi.nlm.nih.gov/32860757/
4. Yamaguchi, Kiyoshi, Nakagawa, Saya, Saku, Akari, Imoto, Seiya, Furukawa, Yoichi. 2023. Bromodomain protein BRD8 regulates cell cycle progression in colorectal cancer cells through a TIP60-independent regulation of the pre-RC complex. In iScience, 26, 106563. doi:10.1016/j.isci.2023.106563. https://pubmed.ncbi.nlm.nih.gov/37123243/
5. Browne, James A, NandyMazumdar, Monali, Paranjapye, Alekh, Leir, Shih-Hsing, Harris, Ann. 2021. The Bromodomain Containing 8 (BRD8) transcriptional network in human lung epithelial cells. In Molecular and cellular endocrinology, 524, 111169. doi:10.1016/j.mce.2021.111169. https://pubmed.ncbi.nlm.nih.gov/33476703/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen