C57BL/6JCya-Ern1em1flox/Cya
Common Name:
Ern1-flox
Product ID:
S-CKO-16909
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ern1-flox
Strain ID
CKOCMP-78943-Ern1-B6J-VA
Gene Name
Product ID
S-CKO-16909
Gene Alias
9030414B18Rik; Ire1a; Ire1alpha; Ire1p
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ern1em1flox/Cya mice (Catalog S-CKO-16909) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001059
NCBI RefSeq
NM_023913
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Ern1, also known as inositol requiring enzyme 1 alpha (IRE1α), is one of the main transducers of the unfolded protein response (UPR) [2]. The UPR is a key intracellular signal transduction pathway activated in response to endoplasmic reticulum (ER) stress, which occurs when misfolded proteins accumulate in the ER lumen [1,3,4,6]. The UPR aims to restore ER homeostasis by regulating the expression of numerous genes, or induce apoptosis if ER stress persists [1,4].
In glioma cells, studies on ERN1 knockdown (a form of loss-of-function experiment) have revealed its role in gene regulation under nutrient deprivations. Glutamine and glucose deprivations affect the expression of pyruvate dehydrogenase genes, homeobox genes, and genes encoding endothelin-1 and its receptors in an ERN1-dependent manner [5,7,8]. For example, ERN1 knockdown modifies the impact of glutamine and glucose deprivations on the expression of PDHA1, PDHB, DLAT, DLD, and PDHX genes [5]. Similarly, it also changes the sensitivity of PAX6, PBX3, PBXIP1, MEIS1, and MEIS2 genes to glucose and glutamine deprivations [7].
In conclusion, Ern1 plays a crucial role in the UPR, which is vital for maintaining ER homeostasis. Through loss-of-function studies in glioma cells, Ern1 has been shown to be involved in regulating gene expression in response to nutrient deprivations. Understanding the function of Ern1 may provide insights into the mechanisms underlying glioma growth and potentially offer new therapeutic strategies for glioma treatment.
References：
1. Walter, Peter, Ron, David. . The unfolded protein response: from stress pathway to homeostatic regulation. In Science (New York, N.Y.), 334, 1081-6. doi:10.1126/science.1209038. https://pubmed.ncbi.nlm.nih.gov/22116877/
2. Raymundo, Diana Pelizzari, Doultsinos, Dimitrios, Guillory, Xavier, Eriksson, Leif A, Chevet, Eric. 2020. Pharmacological Targeting of IRE1 in Cancer. In Trends in cancer, 6, 1018-1030. doi:10.1016/j.trecan.2020.07.006. https://pubmed.ncbi.nlm.nih.gov/32861679/
3. Hwang, Jiwon, Qi, Ling. 2018. Quality Control in the Endoplasmic Reticulum: Crosstalk between ERAD and UPR pathways. In Trends in biochemical sciences, 43, 593-605. doi:10.1016/j.tibs.2018.06.005. https://pubmed.ncbi.nlm.nih.gov/30056836/
4. Hetz, Claudio, Papa, Feroz R. 2017. The Unfolded Protein Response and Cell Fate Control. In Molecular cell, 69, 169-181. doi:10.1016/j.molcel.2017.06.017. https://pubmed.ncbi.nlm.nih.gov/29107536/
5. Shatokhina, Hanna O, Khita, Olena O, Minchenko, Dmytro O, Levadna, Liudmyla O, Minchenko, Oleksandr H. 2022. ERN1 dependent impact of glutamine and glucose deprivations on the pyruvate dehydrogenase genes expression in glioma cells. In Endocrine regulations, 56, 254-264. doi:10.2478/enr-2022-0027. https://pubmed.ncbi.nlm.nih.gov/36270342/
6. Iurlaro, Raffaella, Muñoz-Pinedo, Cristina. 2015. Cell death induced by endoplasmic reticulum stress. In The FEBS journal, 283, 2640-52. doi:10.1111/febs.13598. https://pubmed.ncbi.nlm.nih.gov/26587781/
7. Krasnytska, Dariia O, Viletska, Yuliia M, Minchenko, Dmytro O, Oksiom, Nataliia S, Minchenko, Oleksandr H. 2023. ERN1 dependent impact of glucose and glutamine deprivations on PBX3, PBXIP1, PAX6, MEIS1, and MEIS2 genes expression in U87 glioma cells. In Endocrine regulations, 57, 37-47. doi:10.2478/enr-2023-0005. https://pubmed.ncbi.nlm.nih.gov/36753664/
8. Minchenko, Dmytro O, Khita, Olena O, Tsymbal, Dariia O, Krasnytska, Dariia A, Minchenko, Oleksandr H. 2021. ERN1 knockdown modifies the impact of glucose and glutamine deprivations on the expression of EDN1 and its receptors in glioma cells. In Endocrine regulations, 55, 72-82. doi:10.2478/enr-2021-0009. https://pubmed.ncbi.nlm.nih.gov/34020533/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen