C57BL/6JCya-Golga2em1flox/Cya
Common Name:
Golga2-flox
Product ID:
S-CKO-17369
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Golga2-flox
Strain ID
CKOCMP-99412-Golga2-B6J-VA
Gene Name
Product ID
S-CKO-17369
Gene Alias
GM130
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Golga2em1flox/Cya mice (Catalog S-CKO-17369) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100194
NCBI RefSeq
NM_133852
Target Region
Exon 14
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
GOLGA2, also known as GM130, is a cis-Golgi matrix protein. It plays a crucial role in glycosylation and protein transport in the secretory pathway. GOLGA2 is also involved in vesicle tethering, cell proliferation, and autophagy, and is essential for the assembly of the Golgi apparatus as a single complex [1,2,4,5]. Genetic models, such as gene knockout in mice and zebrafish, have been valuable in studying its functions [5].
In GOLGA2 knockout mice, deletion of GOLGA2 induces autophagy along with Golgi disruption. This autophagy induction leads to fibrosis in the lung and liver, accompanied by a reduction in subcellular lipid storage [3]. In a human patient with a homozygous loss-of-function mutation in GOLGA2, a neuromuscular disorder characterized by developmental delay, seizures, progressive microcephaly, and muscular dystrophy was observed. Knockdown of golga2 in zebrafish recapitulated the human phenotype of severe skeletal muscle disorganization and microcephaly [5]. A second consanguineous family with bi-allelic loss-of-function mutations in GOLGA2 also presented with microcephaly, seizures, and myopathy [6].
In conclusion, GOLGA2 is essential for Golgi function, autophagy regulation, and normal development. The GOLGA2 knockout models in mice and zebrafish, as well as human cases with GOLGA2 mutations, have revealed its role in fibrosis and neuromuscular disorders, providing insights into the underlying mechanisms and potential therapeutic targets for these disease areas.
References:
1. Ding, Xianming, Jiang, Xiao, Tian, Rui, Tang, Zaiming, Sun, Qiming. 2019. RAB2 regulates the formation of autophagosome and autolysosome in mammalian cells. In Autophagy, 15, 1774-1786. doi:10.1080/15548627.2019.1596478. https://pubmed.ncbi.nlm.nih.gov/30957628/
2. Joachim, Justin, Tooze, Sharon A. . GABARAP activates ULK1 and traffics from the centrosome dependent on Golgi partners WAC and GOLGA2/GM130. In Autophagy, 12, 892-3. doi:10.1080/15548627.2016.1159368. https://pubmed.ncbi.nlm.nih.gov/26986052/
3. Park, Sungjin, Kim, Sanghwa, Kim, Min Jung, Park, Jongsun, Cho, Myung-Haing. 2017. GOLGA2 loss causes fibrosis with autophagy in the mouse lung and liver. In Biochemical and biophysical research communications, 495, 594-600. doi:10.1016/j.bbrc.2017.11.049. https://pubmed.ncbi.nlm.nih.gov/29128360/
4. Chang, Seung-Hee, Hong, Seong-Ho, Jiang, Hu-Lin, Chae, Chanhee, Cho, Myung-Haing. 2012. GOLGA2/GM130, cis-Golgi matrix protein, is a novel target of anticancer gene therapy. In Molecular therapy : the journal of the American Society of Gene Therapy, 20, 2052-63. doi:10.1038/mt.2012.125. https://pubmed.ncbi.nlm.nih.gov/22735382/
5. Shamseldin, Hanan E, Bennett, Alexis H, Alfadhel, Majid, Gupta, Vandana, Alkuraya, Fowzan S. 2016. GOLGA2, encoding a master regulator of golgi apparatus, is mutated in a patient with a neuromuscular disorder. In Human genetics, 135, 245-251. doi:10.1007/s00439-015-1632-8. https://pubmed.ncbi.nlm.nih.gov/26742501/
6. Kotecha, Udhaya, Mistri, Mehul, Shah, Nidhi, Shah, Parth S, Gupta, Vandana A. 2021. Bi-allelic loss of function variants in GOLGA2 are associated with a complex neurological phenotype: Report of a second family. In Clinical genetics, 100, 748-751. doi:10.1111/cge.14053. https://pubmed.ncbi.nlm.nih.gov/34424553/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen