C57BL/6JCya-Egln1em1flox/Cya
Common Name:
Egln1-flox
Product ID:
S-CKO-17631
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Egln1-flox
Strain ID
CKOCMP-112405-Egln1-B6J-VC
Gene Name
Product ID
S-CKO-17631
Gene Alias
C1orf12; HIF-PH2; HPH-2; Hif-p4h-2; ORF13; Phd2; SM-20
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Egln1em1flox/Cya mice (Catalog S-CKO-17631) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034469
NCBI RefSeq
NM_053207
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Egln1, also known as PHD2 (prolyl hydroxylase domain-containing protein 2), is a prolyl hydroxylase enzyme. It functions as an oxygen sensor and plays a central role in the hypoxia-inducible factor (HIF)-mediated hypoxia signaling pathway. Under normoxia, Egln1 catalyzes prolyl hydroxylation of HIF-1α, leading to its proteasomal degradation [2].
In breast cancer, Egln1 accumulates on mitochondria under hypoxia. Its β2β3 loop region is responsible for mitochondrial translocation and contributes to tumor growth. Egln1 interacts with AMP-activated protein kinase alpha (AMPKα) on mitochondria, and this interaction is essential for their mutual mitochondrial translocation. Under normoxia, Egln1 prolyl-hydroxylates AMPKα, and they dissociate, while hypoxia leads to their constant interaction and accumulation, activating AMPKα phosphorylation and ensuring metabolic homeostasis and breast tumor growth [1].
In osteocytes, conditional loss of Phd2 (Egln1) in vivo upregulated Fgf23, suggesting Egln1 is a critical mediator of osteocyte FGF23 production, which may provide new therapeutic targets for skeletal diseases involving disturbed oxygen/iron sensing [3].
In nasopharyngeal carcinoma, Egln1 promotes tumorigenesis and radioresistance by facilitating degradation of tumor protein p53 through the ubiquitination system in a hydroxylase-dependent manner [4].
In conclusion, Egln1 is crucial in oxygen sensing and multiple biological processes. Its functions are revealed through in vivo studies such as gene knockout models in various disease areas including breast cancer, skeletal diseases, and nasopharyngeal carcinoma. These models help us understand its role in tumor growth, metabolism, and biomineralization, providing potential therapeutic targets for related diseases.
References:
1. Jiang, Weiwei, Zhang, Mengyao, Gao, Chuan, Zhang, Chen-Song, Zhang, Jing. 2023. A mitochondrial EglN1-AMPKα axis drives breast cancer progression by enhancing metabolic adaptation to hypoxic stress. In The EMBO journal, 42, e113743. doi:10.15252/embj.2023113743. https://pubmed.ncbi.nlm.nih.gov/37661833/
2. Tang, Jinhua, Deng, Hongyan, Wang, Zixuan, Liu, Xing, Xiao, Wuhan. 2022. EGLN1 prolyl hydroxylation of hypoxia-induced transcription factor HIF1α is repressed by SET7-catalyzed lysine methylation. In The Journal of biological chemistry, 298, 101961. doi:10.1016/j.jbc.2022.101961. https://pubmed.ncbi.nlm.nih.gov/35452683/
3. Noonan, Megan L, Ni, Pu, Solis, Emmanuel, Wan, Jun, White, Kenneth E. 2023. Osteocyte Egln1/Phd2 links oxygen sensing and biomineralization via FGF23. In Bone research, 11, 7. doi:10.1038/s41413-022-00241-w. https://pubmed.ncbi.nlm.nih.gov/36650133/
4. Sun, Lu, Wu, Cheng, Ming, Jun, Li, Lingling, Hu, Guoqing. 2022. EGLN1 induces tumorigenesis and radioresistance in nasopharyngeal carcinoma by promoting ubiquitination of p53 in a hydroxylase-dependent manner. In Journal of Cancer, 13, 2061-2073. doi:10.7150/jca.66080. https://pubmed.ncbi.nlm.nih.gov/35517429/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen