Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Mdm2em1flox/Cya
Common Name:
Mdm2-flox
Product ID:
S-CKO-17659
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mdm2-flox
Strain ID
CKOCMP-17246-Mdm2-B6N-VB
Gene Name
Mdm2
Product ID
S-CKO-17659
Gene Alias
1700007J15Rik; Mdm-2
Background
C57BL/6NCya
NCBI ID
17246
Modification
Conditional knockout
Chromosome
10
Phenotype
MGI:96952
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mdm2em1flox/Cya mice (Catalog S-CKO-17659) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020408
NCBI RefSeq
NM_010786
Target Region
Exon 5~6
Size of Effective Region
~1.3 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mdm2, also known as murine double minute 2, is a well-recognized oncogene. It codes for a protein that functions as an E3 ubiquitin ligase, with a key role in targeting the tumor suppressor protein p53 for degradation. This interaction forms a negative feedback loop, as p53 activates the expression of the mdm2 gene, and Mdm2 in turn promotes p53 degradation, thus inhibiting p53's ability to regulate cell cycle progression and apoptosis. Mdm2 is involved in multiple cellular processes such as the cell cycle, apoptosis, DNA repair, and oncogene activation through p53-dependent and p53-independent signaling pathways [2,3,4,5,6,9].

Overexpression/amplification of Mdm2 has been detected in various human cancers and is associated with disease progression, treatment resistance, and poor patient outcomes. It promotes cancer growth through multiple means like growth stimulation, sustained angiogenesis, metabolic reprogramming, apoptosis evasion, metastasis, and immunosuppression. In addition, Mdm2 is involved in tumor immune evasion and can lead to hyperprogressive disease in patients undergoing immune checkpoint inhibitor treatment for malignancies. Mdm2 also has p53-independent functions, such as its role as a chromatin modifier, regulating gene expression similar to polycomb repressive complex 2 (PRC2) [1,4,5,7,8].

In conclusion, Mdm2 is a crucial regulator in cells, with its dysregulation contributing significantly to tumorigenesis and affecting cancer patients' prognosis and response to immunotherapy. Understanding Mdm2's functions through in vivo studies, potentially including gene knockout (KO) or conditional knockout (CKO) mouse models, could provide insights into developing more effective cancer therapies targeting Mdm2 [1,4,5,7].

References:
1. Wang, Wei, Albadari, Najah, Du, Yi, Zhou, Jia, Zhang, Ruiwen. 2024. MDM2 Inhibitors for Cancer Therapy: The Past, Present, and Future. In Pharmacological reviews, 76, 414-453. doi:10.1124/pharmrev.123.001026. https://pubmed.ncbi.nlm.nih.gov/38697854/
2. Haupt, Y, Maya, R, Kazaz, A, Oren, M. . Mdm2 promotes the rapid degradation of p53. In Nature, 387, 296-9. doi:. https://pubmed.ncbi.nlm.nih.gov/9153395/
3. Koo, Nayeong, Sharma, Arun K, Narayan, Satya. 2022. Therapeutics Targeting p53-MDM2 Interaction to Induce Cancer Cell Death. In International journal of molecular sciences, 23, . doi:10.3390/ijms23095005. https://pubmed.ncbi.nlm.nih.gov/35563397/
4. Zafar, Aasma, Khan, Muhammad Jawad, Naeem, Aisha. 2023. MDM2- an indispensable player in tumorigenesis. In Molecular biology reports, 50, 6871-6883. doi:10.1007/s11033-023-08512-3. https://pubmed.ncbi.nlm.nih.gov/37314603/
5. Zeng, Qinru, Zeng, Shaocheng, Dai, Xiaofeng, Tang, Xiaomei, Deng, Jun. 2024. MDM2 inhibitors in cancer immunotherapy: Current status and perspective. In Genes & diseases, 11, 101279. doi:10.1016/j.gendis.2024.101279. https://pubmed.ncbi.nlm.nih.gov/39263534/
6. Sun, Sylvia Yao, Crago, Aimee. 2023. MDM2 Implications for Potential Molecular Pathogenic Therapies of Soft-Tissue Tumors. In Journal of clinical medicine, 12, . doi:10.3390/jcm12113638. https://pubmed.ncbi.nlm.nih.gov/37297833/
7. Sun, Dantong, Qian, Haili, Li, Junling, Xing, Puyuan. 2024. Targeting MDM2 in malignancies is a promising strategy for overcoming resistance to anticancer immunotherapy. In Journal of biomedical science, 31, 17. doi:10.1186/s12929-024-01004-x. https://pubmed.ncbi.nlm.nih.gov/38281981/
8. Wienken, Magdalena, Moll, Ute M, Dobbelstein, Matthias. . Mdm2 as a chromatin modifier. In Journal of molecular cell biology, 9, 74-80. doi:10.1093/jmcb/mjw046. https://pubmed.ncbi.nlm.nih.gov/27927750/
9. Iwakuma, Tomoo, Lozano, Guillermina. . MDM2, an introduction. In Molecular cancer research : MCR, 1, 993-1000. doi:. https://pubmed.ncbi.nlm.nih.gov/14707282/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest