C57BL/6NCya-Mdm2em1flox/Cya
Common Name:
Mdm2-flox
Product ID:
S-CKO-17659
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mdm2-flox
Strain ID
CKOCMP-17246-Mdm2-B6N-VB
Gene Name
Product ID
S-CKO-17659
Gene Alias
1700007J15Rik; Mdm-2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mdm2em1flox/Cya mice (Catalog S-CKO-17659) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020408
NCBI RefSeq
NM_010786
Target Region
Exon 5~6
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Mdm2, also known as murine double minute 2, is a well-recognized oncogene. It codes for a protein that functions as an E3 ubiquitin ligase, with a key role in targeting the tumor suppressor protein p53 for degradation. This interaction forms a negative feedback loop, as p53 activates the expression of the mdm2 gene, and Mdm2 in turn promotes p53 degradation, thus inhibiting p53's ability to regulate cell cycle progression and apoptosis. Mdm2 is involved in multiple cellular processes such as the cell cycle, apoptosis, DNA repair, and oncogene activation through p53-dependent and p53-independent signaling pathways [2,3,4,5,6,9].
Overexpression/amplification of Mdm2 has been detected in various human cancers and is associated with disease progression, treatment resistance, and poor patient outcomes. It promotes cancer growth through multiple means like growth stimulation, sustained angiogenesis, metabolic reprogramming, apoptosis evasion, metastasis, and immunosuppression. In addition, Mdm2 is involved in tumor immune evasion and can lead to hyperprogressive disease in patients undergoing immune checkpoint inhibitor treatment for malignancies. Mdm2 also has p53-independent functions, such as its role as a chromatin modifier, regulating gene expression similar to polycomb repressive complex 2 (PRC2) [1,4,5,7,8].
In conclusion, Mdm2 is a crucial regulator in cells, with its dysregulation contributing significantly to tumorigenesis and affecting cancer patients' prognosis and response to immunotherapy. Understanding Mdm2's functions through in vivo studies, potentially including gene knockout (KO) or conditional knockout (CKO) mouse models, could provide insights into developing more effective cancer therapies targeting Mdm2 [1,4,5,7].
References:
1. Wang, Wei, Albadari, Najah, Du, Yi, Zhou, Jia, Zhang, Ruiwen. 2024. MDM2 Inhibitors for Cancer Therapy: The Past, Present, and Future. In Pharmacological reviews, 76, 414-453. doi:10.1124/pharmrev.123.001026. https://pubmed.ncbi.nlm.nih.gov/38697854/
2. Haupt, Y, Maya, R, Kazaz, A, Oren, M. . Mdm2 promotes the rapid degradation of p53. In Nature, 387, 296-9. doi:. https://pubmed.ncbi.nlm.nih.gov/9153395/
3. Koo, Nayeong, Sharma, Arun K, Narayan, Satya. 2022. Therapeutics Targeting p53-MDM2 Interaction to Induce Cancer Cell Death. In International journal of molecular sciences, 23, . doi:10.3390/ijms23095005. https://pubmed.ncbi.nlm.nih.gov/35563397/
4. Zafar, Aasma, Khan, Muhammad Jawad, Naeem, Aisha. 2023. MDM2- an indispensable player in tumorigenesis. In Molecular biology reports, 50, 6871-6883. doi:10.1007/s11033-023-08512-3. https://pubmed.ncbi.nlm.nih.gov/37314603/
5. Zeng, Qinru, Zeng, Shaocheng, Dai, Xiaofeng, Tang, Xiaomei, Deng, Jun. 2024. MDM2 inhibitors in cancer immunotherapy: Current status and perspective. In Genes & diseases, 11, 101279. doi:10.1016/j.gendis.2024.101279. https://pubmed.ncbi.nlm.nih.gov/39263534/
6. Sun, Sylvia Yao, Crago, Aimee. 2023. MDM2 Implications for Potential Molecular Pathogenic Therapies of Soft-Tissue Tumors. In Journal of clinical medicine, 12, . doi:10.3390/jcm12113638. https://pubmed.ncbi.nlm.nih.gov/37297833/
7. Sun, Dantong, Qian, Haili, Li, Junling, Xing, Puyuan. 2024. Targeting MDM2 in malignancies is a promising strategy for overcoming resistance to anticancer immunotherapy. In Journal of biomedical science, 31, 17. doi:10.1186/s12929-024-01004-x. https://pubmed.ncbi.nlm.nih.gov/38281981/
8. Wienken, Magdalena, Moll, Ute M, Dobbelstein, Matthias. . Mdm2 as a chromatin modifier. In Journal of molecular cell biology, 9, 74-80. doi:10.1093/jmcb/mjw046. https://pubmed.ncbi.nlm.nih.gov/27927750/
9. Iwakuma, Tomoo, Lozano, Guillermina. . MDM2, an introduction. In Molecular cancer research : MCR, 1, 993-1000. doi:. https://pubmed.ncbi.nlm.nih.gov/14707282/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen