C57BL/6JCya-Pkmem1flox/Cya
Common Name:
Pkm-flox
Product ID:
S-CKO-17686
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pkm-flox
Strain ID
CKOCMP-18746-Pkm-B6J-VE
Gene Name
Product ID
S-CKO-17686
Gene Alias
Pk-2; Pk-3; Pk3; Pkm2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pkmem1flox/Cya mice (Catalog S-CKO-17686) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034834
NCBI RefSeq
NM_011099.4
Target Region
Exon 9 of Pkm (PKM2 Isoform Specific)
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Pkm, encoding pyruvate kinase muscle isoforms, is crucial in the glycolytic pathway. Pyruvate kinase catalyzes the final and rate-limiting step of glycolysis, converting Phosphoenolpyruvate (PEP) to Pyruvate. PKM1 and PKM2 are formed by alternative splicing of the PKM gene transcript. PKM2, upregulated in most cancers, is involved in the Warburg effect, where cancer cells prefer aerobic glycolysis over oxidative phosphorylation for energy metabolism [1,3,4].
In a genetic HCC mouse model, a surrogate mouse-specific ASO induced Pkm splice-switching from the cancer-associated PKM2 to the PKM1 isoform, inhibiting tumorigenesis without observable toxicity. This shows that altering Pkm splicing can be a potential therapeutic strategy for HCC [1]. In colorectal cancer, the lncRNA LINC01852 inhibits SRSF5-mediated alternative splicing of Pkm, decreasing the production of PKM2 and attenuating chemoresistance [2].
In conclusion, Pkm, through its alternative splicing products PKM1 and PKM2, plays a vital role in energy metabolism, especially in the context of cancer. The use of genetic mouse models, like the HCC mouse model, has revealed the potential of targeting Pkm splicing as a therapeutic approach for cancer, highlighting the importance of Pkm in cancer-related metabolic reprogramming.
References:
1. Ma, Wai Kit, Voss, Dillon M, Scharner, Juergen, Bennett, C Frank, Krainer, Adrian R. . ASO-Based PKM Splice-Switching Therapy Inhibits Hepatocellular Carcinoma Growth. In Cancer research, 82, 900-915. doi:10.1158/0008-5472.CAN-20-0948. https://pubmed.ncbi.nlm.nih.gov/34921016/
2. Bian, Zehua, Yang, Fan, Xu, Peiwen, Fei, Bojian, Huang, Zhaohui. 2024. LINC01852 inhibits the tumorigenesis and chemoresistance in colorectal cancer by suppressing SRSF5-mediated alternative splicing of PKM. In Molecular cancer, 23, 23. doi:10.1186/s12943-024-01939-7. https://pubmed.ncbi.nlm.nih.gov/38263157/
3. Li, Yuchao, Zhang, Shuwei, Li, Yuexian, Zang, Wenli, Pan, Yaping. 2024. The Regulatory Network of hnRNPs Underlying Regulating PKM Alternative Splicing in Tumor Progression. In Biomolecules, 14, . doi:10.3390/biom14050566. https://pubmed.ncbi.nlm.nih.gov/38785973/
4. Zahra, Kulsoom, Dey, Tulika, Mishra, Surendra Pratap, Pandey, Uma. 2020. Pyruvate Kinase M2 and Cancer: The Role of PKM2 in Promoting Tumorigenesis. In Frontiers in oncology, 10, 159. doi:10.3389/fonc.2020.00159. https://pubmed.ncbi.nlm.nih.gov/32195169/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen