C57BL/6JCya-Crygsem1flox/Cya
Common Name:
Crygs-flox
Product ID:
S-CKO-17897
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Crygs-flox
Strain ID
CKOCMP-12970-Crygs-B6J-VB
Gene Name
Product ID
S-CKO-17897
Gene Alias
Opj; rncat
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crygsem1flox/Cya mice (Catalog S-CKO-17897) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040592
NCBI RefSeq
NM_009967.2
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Crygs, the gene for gammaS-crystallin, is crucial for maintaining lens transparency. GammaS-crystallin is a monomeric betagamma-crystallin, which is composed of two paired homologous domains, each with two Greek key motifs [4]. It may also have non-lens functions, as its expression has been detected in non-lens tissues such as the retina and cornea, potentially acting as a stress-related protein in the eye [5].
Mutations in Crygs are associated with various cataract-related phenotypes. In a Chinese family, a G57W mutation of CRYGS was found to be associated with autosomal dominant pulverulent cataracts [1]. A missense mutation c.199T>A, p.(Tyr67Asn) in CRYGS was identified in a five-generation Chinese family with autosomal dominant nuclear congenital cataracts, and this substitution was predicted to affect the protein's structure and translocation [2]. In mice, a temperature-sensitive mutation of Crygs in the Opj cataract led to disrupted fiber cell morphology and loss of maturing fiber cell nuclei, suggesting a loss of gammaS-crystallin function in maintaining cytoarchitecture [3]. The G18V mutation in human gammaS-crystallin associated with autosomal dominant cortical progressive cataract was shown to increase the protein's sensitivity to thermal and chemical stress, consistent with progressive cataract formation in family members carrying this mutation [4]. In Crygs-mutated cataractous mice, the mutant gene led to changes in BFSP/filensin and other crystallins, which may secondarily lead to cataract formation [6].
In conclusion, Crygs plays a vital role in maintaining lens transparency and the cytoarchitecture of the lens. Research on Crygs-mutated mouse models has significantly contributed to understanding the molecular mechanisms underlying cataract formation, providing insights into the genetic basis of this common eye disorder.
References:
1. Yang, Zhenfei, Li, Qian, Zhu, Siquan, Ma, Xu. . A G57W Mutation of CRYGS Associated with Autosomal Dominant Pulverulent Cataracts in a Chinese Family. In Ophthalmic genetics, 36, 281-3. doi:10.3109/13816810.2013.865761. https://pubmed.ncbi.nlm.nih.gov/24328668/
2. Zhang, Tianxiao, Yan, Lulu, Leng, Yunji, Zhang, Jinsong, Cao, Lihua. 2018. A novel missense mutation of CRYGS underlies congenital cataract in a Chinese family. In Gene, 675, 9-14. doi:10.1016/j.gene.2018.06.100. https://pubmed.ncbi.nlm.nih.gov/29964096/
3. Sinha, D, Wyatt, M K, Sarra, R, Lyon, M, Wistow, G. 2000. A temperature-sensitive mutation of Crygs in the murine Opj cataract. In The Journal of biological chemistry, 276, 9308-15. doi:. https://pubmed.ncbi.nlm.nih.gov/11121426/
4. Ma, Zhiwei, Piszczek, Grzegorz, Wingfield, Paul T, Sergeev, Yuri V, Hejtmancik, J Fielding. . The G18V CRYGS mutation associated with human cataracts increases gammaS-crystallin sensitivity to thermal and chemical stress. In Biochemistry, 48, 7334-41. doi:10.1021/bi900467a. https://pubmed.ncbi.nlm.nih.gov/19558189/
5. Sinha, D, Esumi, N, Jaworski, C, Pierce, E, Wistow, G. 1998. Cloning and mapping the mouse Crygs gene and non-lens expression of [gamma]S-crystallin. In Molecular vision, 4, 8. doi:. https://pubmed.ncbi.nlm.nih.gov/9565648/
6. Ji, Yinghong, Bi, Hua, Li, Na, Yan, Shunsheng, Lu, Yi. 2010. Alterations to proteins in the lens of hereditary Crygs-mutated cataractous mice. In Molecular vision, 16, 1068-75. doi:. https://pubmed.ncbi.nlm.nih.gov/20596256/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen