C57BL/6JCya-Lpcat2em1flox/Cya
Common Name:
Lpcat2-flox
Product ID:
S-CKO-17929
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lpcat2-flox
Strain ID
CKOCMP-270084-Lpcat2-B6J-VB
Gene Name
Product ID
S-CKO-17929
Gene Alias
1-AGPAT 11; A330042H22; Aytl1; Aytl1a; lpafat1; lysoPAFAT; lysoPAFAT/LPCAT2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lpcat2em1flox/Cya mice (Catalog S-CKO-17929) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046290
NCBI RefSeq
NM_173014
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Lpcat2, lysophosphatidylcholine acyltransferase 2, is an enzyme involved in phosphatidylcholine synthesis. It contributes to the biosynthesis of polyunsaturated fatty acid-containing phospholipids, which are substrates for lipid peroxidation. It is also associated with lipid droplet production, and plays roles in regulating inflammatory gene expression, and may be involved in circadian-related metabolic pathways [2,3,4,5]. Genetic models, such as knockout mice, are valuable for studying its functions.
In a Lpcat2 knockout mouse (Lpcat2-/-) colon cancer model, Lpcat2-/-mice showed more severe lesions. Lpcat2 was found to inhibit the proliferation of colorectal cancer cells by inducing ferroptosis. It arrested PRMT1 in the cytoplasm of CRC cells, attenuating the positive regulatory effect of PRMT1 on SLC7A11 promoter, which is a ferroptosis regulator [1]. In pancreatic cancer, loss of Lpcat2 repressed lipid droplet accumulation, gemcitabine resistance, and cell motility, and enhanced chemotherapy sensitivity in a xenograft model [3].
In conclusion, Lpcat2 has diverse functions including in lipid-related processes and ferroptosis regulation. The Lpcat2 knockout mouse models have revealed its significance in colorectal and pancreatic cancer progression, suggesting it could be a potential therapeutic target in these disease areas.
References：
1. Cao, Nan, Zhang, Fangmei, Yin, Jiang, Lin, Ying, Luo, Liyun. 2024. LPCAT2 inhibits colorectal cancer progression via the PRMT1/SLC7A11 axis. In Oncogene, 43, 1714-1725. doi:10.1038/s41388-024-02996-4. https://pubmed.ncbi.nlm.nih.gov/38605214/
2. Merkel, Melanie, Goebel, Bjarne, Boll, Moritz, Steinhilber, Dieter, Culmsee, Carsten. 2023. Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis. In Antioxidants (Basel, Switzerland), 12, . doi:10.3390/antiox12081590. https://pubmed.ncbi.nlm.nih.gov/37627584/
3. Lin, Yuhe, Zhang, Xin, Wang, Yihui, Yao, Wei. 2024. LPCAT2-mediated lipid droplet production supports pancreatic cancer chemoresistance and cell motility. In International immunopharmacology, 139, 112681. doi:10.1016/j.intimp.2024.112681. https://pubmed.ncbi.nlm.nih.gov/39068758/
4. Wang, Qingqing, Zhou, Shuning, Hu, Xinyi, Gao, Yu, Li, Shujing. 2023. Circadian Genes MBOAT2/CDA/LPCAT2/B4GALT5 in the Metabolic Pathway Serve as New Biomarkers of PACA Prognosis and Immune Infiltration. In Life (Basel, Switzerland), 13, . doi:10.3390/life13051116. https://pubmed.ncbi.nlm.nih.gov/37240761/
5. Abate, W, Alrammah, H, Kiernan, M, Tonks, A J, Jackson, S K. 2020. Lysophosphatidylcholine acyltransferase 2 (LPCAT2) co-localises with TLR4 and regulates macrophage inflammatory gene expression in response to LPS. In Scientific reports, 10, 10355. doi:10.1038/s41598-020-67000-x. https://pubmed.ncbi.nlm.nih.gov/32587324/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen