C57BL/6JCya-Miosem1flox/Cya
Common Name:
Mios-flox
Product ID:
S-CKO-18036
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mios-flox
Strain ID
CKOCMP-252875-Mios-B6J-VB
Gene Name
Product ID
S-CKO-18036
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Miosem1flox/Cya mice (Catalog S-CKO-18036) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040017
NCBI RefSeq
NM_145374
Target Region
Exon 3~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Mios is a component of the GATOR2 complex, which is crucial for amino acids to activate mTORC1, a key regulator controlling growth by modulating anabolic and catabolic processes in response to environmental cues [1]. The GATOR2-GATOR1 signaling axis is essential for amino-acid-dependent mTORC1 activation [3]. In zebrafish, Rbpms2 promotes female fate upstream of Mios, integrating the regulation of sexual differentiation factors and nutritional availability pathways during oogenesis [2].
Disruption of the Ring domains of Mios completely impedes amino-acid-mediated mTORC1 activation. Mechanistically, the Ring domain of Mios acts as a hub to maintain GATOR2 integrity; its disruption leads to self-ubiquitination of WDR24. Physiologically, leucine stimulation dissociates Sestrin2 from the Ring domain of WDR24, contributing to GATOR2-mediated GATOR1 inactivation. WDR24 ablation or Ring deletion in mice, which can be considered as a form of loss-of-function, prevents mTORC1 activation, leading to severe growth defects and embryonic lethality at E10.5 [3].
In conclusion, Mios is essential for the proper function of the GATOR2 complex in the mTORC1 pathway, playing a key role in nutrient sensing and embryonic development. The loss-of-function experiments in mice highlight the significance of Mios in maintaining normal growth and development, and further suggest its potential implications in understanding related growth-and development-associated diseases.
References:
1. Valenstein, Max L, Rogala, Kacper B, Lalgudi, Pranav V, Quast, Jan-Philipp, Sabatini, David M. 2022. Structure of the nutrient-sensing hub GATOR2. In Nature, 607, 610-616. doi:10.1038/s41586-022-04939-z. https://pubmed.ncbi.nlm.nih.gov/35831510/
2. Wilson, Miranda L, Romano, Shannon N, Khatri, Nitya, Draper, Bruce W, Marlow, Florence L. 2024. Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component Mios. In Nature communications, 15, 5248. doi:10.1038/s41467-024-49613-2. https://pubmed.ncbi.nlm.nih.gov/38898112/
3. Jiang, Cong, Dai, Xiaoming, He, Shaohui, Xiao, Jianru, Wei, Wenyi. 2022. Ring domains are essential for GATOR2-dependent mTORC1 activation. In Molecular cell, 83, 74-89.e9. doi:10.1016/j.molcel.2022.11.021. https://pubmed.ncbi.nlm.nih.gov/36528027/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen