C57BL/6JCya-Ahctf1em1flox/Cya
Common Name:
Ahctf1-flox
Product ID:
S-CKO-18060
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ahctf1-flox
Strain ID
CKOCMP-226747-Ahctf1-B6J-VB
Gene Name
Product ID
S-CKO-18060
Gene Alias
6230412P20Rik; Elys
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ahctf1em1flox/Cya mice (Catalog S-CKO-18060) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027768
NCBI RefSeq
NM_026375
Target Region
Exon 4~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Ahctf1, also known as ELYS, encodes a nucleoporin protein that has essential roles in nuclear pore assembly, mitosis, and is involved in regulating zygotic genome activation (ZGA) [1,2]. It participates in pathways such as WNT signaling-mediated oncogenic MYC expression through super-enhancer-mediated gene gating [3]. Its function is crucial for normal embryonic development and cell cycle-related processes [1,4]. Genetic models like zebrafish are valuable for studying Ahctf1.
In zebrafish, deficiency or dysfunction of Ahctf1 impairs nuclear pore complex (NPC) assembly, maternal transcription factors' nuclear transport, and the onset of ZGA, suggesting its role as a regulator of ZGA [1]. In a zebrafish model of hepatocellular carcinoma (HCC), reducing ahctf1 gene dosage by 50% decreases liver volume, as it impairs nuclear pore formation, mitotic spindle assembly, and chromosome segregation, leading to DNA damage and activation of a Tp53-dependent transcriptional programme [2].
In conclusion, Ahctf1 is essential for nuclear pore assembly, mitosis, and ZGA. Its study in zebrafish models has revealed its significance in embryonic development and cancer, specifically HCC, providing insights into potential therapeutic targets for cancer treatment.
References：
1. Shen, Weimin, Gong, Bo, Xing, Cencan, Wu, Xiaotong, Meng, Anming. 2022. Comprehensive maturity of nuclear pore complexes regulates zygotic genome activation. In Cell, 185, 4954-4970.e20. doi:10.1016/j.cell.2022.11.011. https://pubmed.ncbi.nlm.nih.gov/36493774/
2. Morgan, Kimberly J, Doggett, Karen, Geng, Fansuo, Gong, Zhiyuan, Heath, Joan K. 2023. ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma. In eLife, 12, . doi:10.7554/eLife.73407. https://pubmed.ncbi.nlm.nih.gov/36648336/
3. Scholz, Barbara A, Sumida, Noriyuki, de Lima, Carolina Diettrich Mallet, Göndör, Anita, Ohlsson, Rolf. 2019. WNT signaling and AHCTF1 promote oncogenic MYC expression through super-enhancer-mediated gene gating. In Nature genetics, 51, 1723-1731. doi:10.1038/s41588-019-0535-3. https://pubmed.ncbi.nlm.nih.gov/31784729/
4. Cerveny, Kara L, Bronstein, Hannah, Hagen, Olivia, Welch, Evan, Varga, Máté. 2021. Mutations linked to loss of cell cycle control can render cells responsive to local differentiation cues. In microPublication biology, 2021, . doi:10.17912/micropub.biology.000481. https://pubmed.ncbi.nlm.nih.gov/34723143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen