C57BL/6JCya-Anxa10em1flox/Cya
Common Name:
Anxa10-flox
Product ID:
S-CKO-18124
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Anxa10-flox
Strain ID
CKOCMP-26359-Anxa10-B6J-VB
Gene Name
Product ID
S-CKO-18124
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Anxa10em1flox/Cya mice (Catalog S-CKO-18124) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034052
NCBI RefSeq
NM_001136089
Target Region
Exon 4~5
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Anxa10, a member of the annexin family, is a membrane-bound calcium-dependent phospholipid-binding protein. Although its exact function remains unclear in some aspects, it is involved in multiple biological processes and disease-related pathways [1,2]. Mouse models with Anxa10-specific CreERT2 have been used to study human gastric cancer subtypes, highlighting its potential as a research model for in-vivo studies on gastric cancer [4].
In cancer research, knockdown of Anxa10 in colorectal cancer cells induced ferroptosis by inhibiting autophagy-mediated TFRC degradation, thus suppressing CRC progression [1]. In melanoma, Anxa10 knockout reduced cell migration and metastatic activity, and regulated the N-to E-cadherin switch through the ANXA10-PKD1-SMAD6 axis [2]. In hepatocellular carcinoma, Anxa10 up-regulation inhibited cell proliferation and migration [3]. In papillary thyroid carcinoma, Anxa10 knockdown inhibited cell proliferation, promoted apoptosis, and inactivated the MAPK/ERK signaling pathway by down-regulating TSG101 [7]. In bladder cancer, low Anxa10 expression was associated with shorter progression-free survival and disease aggressiveness [8]. In upper and lower urothelial carcinoma, Anxa10 expression was inversely associated with tumor stage, grade, and TP53 expression [5]. In esophageal squamous cell carcinoma, ANXA10 induced by interaction with tumor-associated macrophages promoted cell growth [6].
In conclusion, Anxa10 is involved in multiple biological functions, especially in various cancer-related processes. Gene knockout or knockdown experiments, including those in KO mouse models, have revealed its roles in tumor progression, metastasis, and ferroptosis. These findings contribute to understanding the mechanisms of diseases such as colorectal, melanoma, liver, thyroid, bladder, urothelial, and esophageal cancers, potentially providing new targets for therapeutic strategies.
References:
1. Wang, Xinyuan, Zhou, Yujie, Ning, Lijun, Chen, Huimin, Li, Xiaobo. 2023. Knockdown of ANXA10 induces ferroptosis by inhibiting autophagy-mediated TFRC degradation in colorectal cancer. In Cell death & disease, 14, 588. doi:10.1038/s41419-023-06114-2. https://pubmed.ncbi.nlm.nih.gov/37666806/
2. Zhang, Xuerui, Hu, Zhaoqing, Wang, Xinran, Zhang, Jing, Hua, Zichun. 2021. ANXA10 promotes melanoma metastasis by suppressing E3 ligase TRIM41-directed PKD1 degradation. In Cancer letters, 519, 237-249. doi:10.1016/j.canlet.2021.07.033. https://pubmed.ncbi.nlm.nih.gov/34324862/
3. Zhang, Chaohua, Peng, Linglong, Gu, Haitao, Wang, Yaxu, Xu, Zhiquan. 2023. ANXA10 is a prognostic biomarker and suppressor of hepatocellular carcinoma: a bioinformatics analysis and experimental validation. In Scientific reports, 13, 1583. doi:10.1038/s41598-023-28527-x. https://pubmed.ncbi.nlm.nih.gov/36709331/
4. Seidlitz, Therese, Chen, Yi-Ting, Uhlemann, Heike, Koo, Bon-Kyoung, Stange, Daniel E. 2019. Mouse Models of Human Gastric Cancer Subtypes With Stomach-Specific CreERT2-Mediated Pathway Alterations. In Gastroenterology, 157, 1599-1614.e2. doi:10.1053/j.gastro.2019.09.026. https://pubmed.ncbi.nlm.nih.gov/31585123/
5. Kobayashi, Go, Hayashi, Tetsutaro, Sentani, Kazuhiro, Hinata, Nobuyuki, Oue, Naohide. 2022. ANXA10 Expression Is Inversely Associated with Tumor Stage, Grade, and TP53 Expression in Upper and Lower Urothelial Carcinoma. In Pathobiology : journal of immunopathology, molecular and cellular biology, 90, 94-103. doi:10.1159/000524989. https://pubmed.ncbi.nlm.nih.gov/35780773/
6. Kodaira, Himiko, Koma, Yu-Ichiro, Hosono, Masayoshi, Shigeoka, Manabu, Yokozaki, Hiroshi. 2019. ANXA10 induction by interaction with tumor-associated macrophages promotes the growth of esophageal squamous cell carcinoma. In Pathology international, 69, 135-147. doi:10.1111/pin.12771. https://pubmed.ncbi.nlm.nih.gov/30758105/
7. Wei, Tianjun, Zhu, Xiangming. 2021. Knockdown of ANXA10 inhibits proliferation and promotes apoptosis of papillary thyroid carcinoma cells by down-regulating TSG101 thereby inactivating the MAPK/ERK signaling pathway. In Journal of bioenergetics and biomembranes, 53, 429-440. doi:10.1007/s10863-021-09902-7. https://pubmed.ncbi.nlm.nih.gov/34032966/
8. Munksgaard, P P, Mansilla, F, Brems Eskildsen, A-S, Orntoft, T F, Dyrskjøt, L. 2011. Low ANXA10 expression is associated with disease aggressiveness in bladder cancer. In British journal of cancer, 105, 1379-87. doi:10.1038/bjc.2011.404. https://pubmed.ncbi.nlm.nih.gov/21979422/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen