C57BL/6JCya-Tfcp2l1em1flox/Cya
Common Name:
Tfcp2l1-flox
Product ID:
S-CKO-18215
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Tfcp2l1-flox
Strain ID
CKOCMP-81879-Tfcp2l1-B6J-VB
Gene Name
Product ID
S-CKO-18215
Gene Alias
1810030F05Rik; 4932442M07Rik; Cp2l1; Crtr-1; D930018N21Rik; LBP-9; Tcfcp2l1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tfcp2l1em1flox/Cya mice (Catalog S-CKO-18215) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027629
NCBI RefSeq
NM_023755
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Tfcp2l1, also known as CRTR-1 and LBP-9, is a transcription factor. It is crucial for maintaining the pluripotency and self-renewal of embryonic stem cells [1,3,6,8,9,10]. It is involved in multiple pathways related to cell fate determination and stemness regulation, and is of great biological importance in processes such as development and tissue regeneration [5]. Genetic models, especially KO/CKO mouse models, are valuable tools for studying its functions.
In renal medullary carcinoma, the loss of renal epithelial transcription factor TFCP2L1 is associated with the transformation of thick ascending limb cells into RMC cells, along with the gain of MYC and NFE2L2-associated oncogenic and ferroptosis resistance programs [2]. In hepatocellular carcinoma, knockdown of TFCP2L1 significantly reduced HCC cell proliferation, invasion, etc., while overexpression enhanced these functions, and it promotes the stemness of cancer stem cells through the NANOG/STAT3 pathway [3]. In thyroid cancer, decreased TFCP2L1 expression was associated with malignant clinicopathological characteristics, and its overexpression inhibited cell growth and motility [4]. In lung alveolar regeneration, Tfcp2l1 loss in adult alveolar epithelial type 2 (AT2) cells inhibits self-renewal and enhances AT2-AT1 differentiation during tissue regeneration [5]. In bladder cancer, the CDK1/TFCP2L1/ID2 cascade is involved in regulating bladder carcinogenesis, and TFCP2L1 downregulates ID2 by directly binding to its promoter region [7]. In mouse embryonic fibroblasts, hypoxia-induced Tfcp2l1 expression contributes to immortalization prior to malignant transformation [10].
In conclusion, Tfcp2l1 plays essential roles in maintaining stem cell pluripotency, self-renewal, and in processes like tissue regeneration. Its dysregulation is implicated in various cancers, including renal medullary carcinoma, hepatocellular carcinoma, thyroid cancer, and bladder cancer. Studies using KO/CKO mouse models have been instrumental in revealing its functions in these disease-related biological processes, providing insights into potential therapeutic targets.
References：
1. Kotarba, Grzegorz, Krzywinska, Ewa, Grabowska, Anna I, Taracha, Agnieszka, Wilanowski, Tomasz. 2018. TFCP2/TFCP2L1/UBP1 transcription factors in cancer. In Cancer letters, 420, 72-79. doi:10.1016/j.canlet.2018.01.078. https://pubmed.ncbi.nlm.nih.gov/29410248/
2. Vokshi, Bujamin H, Davidson, Guillaume, Tawanaie Pour Sedehi, Nassim, Davidson, Irwin, Malouf, Gabriel G. 2023. SMARCB1 regulates a TFCP2L1-MYC transcriptional switch promoting renal medullary carcinoma transformation and ferroptosis resistance. In Nature communications, 14, 3034. doi:10.1038/s41467-023-38472-y. https://pubmed.ncbi.nlm.nih.gov/37236926/
3. Qiu, Dongbo, Wang, Tiantian, Xiong, Yi, Zhang, Qi, Jia, Changchang. 2024. TFCP2L1 drives stemness and enhances their resistance to Sorafenib treatment by modulating the NANOG/STAT3 pathway in hepatocellular carcinoma. In Oncogenesis, 13, 33. doi:10.1038/s41389-024-00534-1. https://pubmed.ncbi.nlm.nih.gov/39266516/
4. Zeng, C, Zhang, Y, Lin, C, Li, Y, Guan, H. 2024. TFCP2L1, a potential differentiation regulator, predicts favorable prognosis and dampens thyroid cancer progression. In Journal of endocrinological investigation, 47, 2953-2968. doi:10.1007/s40618-024-02392-5. https://pubmed.ncbi.nlm.nih.gov/38753296/
5. Cardenas-Diaz, Fabian L, Liberti, Derek C, Leach, John P, Morley, Michael P, Morrisey, Edward E. 2023. Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1. In Cell reports, 42, 112451. doi:10.1016/j.celrep.2023.112451. https://pubmed.ncbi.nlm.nih.gov/37119134/
6. Sun, Hongwei, You, Yu, Guo, Mengmeng, Zhang, Yan, Ye, Shoudong. 2018. Tfcp2l1 safeguards the maintenance of human embryonic stem cell self-renewal. In Journal of cellular physiology, 233, 6944-6951. doi:10.1002/jcp.26483. https://pubmed.ncbi.nlm.nih.gov/29323720/
7. Heo, Jinbeom, Lee, Jinyoung, Nam, Yun Ji, Son, Jaekyoung, Shin, Dong-Myung. 2022. The CDK1/TFCP2L1/ID2 cascade offers a novel combination therapy strategy in a preclinical model of bladder cancer. In Experimental & molecular medicine, 54, 801-811. doi:10.1038/s12276-022-00786-0. https://pubmed.ncbi.nlm.nih.gov/35729325/
8. Zhang, Yan, Ding, Huiwen, Wang, Xiaoxiao, Gan, Ruoyi, Ye, Shou-Dong. . MK2 promotes Tfcp2l1 degradation via β-TrCP ubiquitin ligase to regulate mouse embryonic stem cell self-renewal. In Cell reports, 37, 109949. doi:10.1016/j.celrep.2021.109949. https://pubmed.ncbi.nlm.nih.gov/34731635/
9. Heo, Jinbeom, Noh, Byeong-Joo, Lee, Seungun, Cho, Yong Mee, Shin, Dong-Myung. 2019. Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis. In EMBO molecular medicine, 12, e10880. doi:10.15252/emmm.201910880. https://pubmed.ncbi.nlm.nih.gov/31709755/
10. Otero-Albiol, D, Santos-Pereira, J M, Lucena-Cacace, A, Yoshida, Y, Carnero, A. 2024. Hypoxia-induced immortalization of primary cells depends on Tfcp2L1 expression. In Cell death & disease, 15, 177. doi:10.1038/s41419-024-06567-z. https://pubmed.ncbi.nlm.nih.gov/38418821/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen