C57BL/6JCya-Polrmtem1flox/Cya
Common Name:
Polrmt-flox
Product ID:
S-CKO-18394
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Polrmt-flox
Strain ID
CKOCMP-216151-Polrmt-B6J-VB
Gene Name
Product ID
S-CKO-18394
Gene Alias
1110018N15Rik; 4932416K13; mtRPOL
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Polrmtem1flox/Cya mice (Catalog S-CKO-18394) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020580
NCBI RefSeq
NM_172551
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
POLRMT, the mitochondrial RNA polymerase, is crucial for the transcription of the mitochondrial genome. It plays an essential role in mitochondrial biogenesis, as it is responsible for synthesizing the RNA transcripts that encode key proteins involved in the electron transport chain (ETC), a vital pathway for energy production in cells [2]. Additionally, POLRMT is also essential for mammalian mitochondrial DNA replication, being indispensable for the maintenance of human mtDNA [7].
Mutations in POLRMT can cause neurological diseases. Patients with POLRMT variants present with global developmental delay, hypotonia, short stature, and speech/intellectual disability in childhood; some may also display progressive external ophthalmoplegia. Functional studies show that patient fibroblasts have defective mitochondrial mRNA synthesis [1]. In cancer, POLRMT is overexpressed in various types such as colorectal, prostate, and lung adenocarcinoma. Inhibiting POLRMT using small-molecule inhibitors like IMT1 disrupts mitochondrial functions in cancer cells, leading to mitochondrial depolarization, oxidative damage, decreased ATP levels, and ultimately inhibiting cancer cell growth both in vitro and in vivo [3,4,5,6]. Genetic depletion of POLRMT in cancer cells also impairs mitochondrial functions and suppresses cell viability, proliferation, and migration [4].
In conclusion, POLRMT is essential for mitochondrial transcription and DNA replication. Studies using loss-of-function models, such as genetic depletion in cancer cells and the analysis of POLRMT-mutated patients, have revealed its significant role in neurological diseases and cancer. These findings provide potential therapeutic targets for treating related diseases by targeting POLRMT-mediated mitochondrial functions.
References:
1. Oláhová, Monika, Peter, Bradley, Szilagyi, Zsolt, Gustafsson, Claes M, Taylor, Robert W. 2021. POLRMT mutations impair mitochondrial transcription causing neurological disease. In Nature communications, 12, 1135. doi:10.1038/s41467-021-21279-0. https://pubmed.ncbi.nlm.nih.gov/33602924/
2. Yan, Weiwei, Xie, Chengmei, Sun, Sijun, Xu, Shuangnian, Wang, Yi-Ping. 2024. SUCLG1 restricts POLRMT succinylation to enhance mitochondrial biogenesis and leukemia progression. In The EMBO journal, 43, 2337-2367. doi:10.1038/s44318-024-00101-9. https://pubmed.ncbi.nlm.nih.gov/38649537/
3. Wang, Hao, Liu, Yuxin, Lu, Xing-Sheng, Gu, Wen, Yin, Guojian. 2024. Targeting POLRMT by IMT1 inhibits colorectal cancer cell growth. In Cell death & disease, 15, 643. doi:10.1038/s41419-024-07023-8. https://pubmed.ncbi.nlm.nih.gov/39227564/
4. Li, Xiaojun, Yao, Linya, Wang, Tao, Wu, Yufan, Jiang, Ting. 2023. Identification of the mitochondrial protein POLRMT as a potential therapeutic target of prostate cancer. In Cell death & disease, 14, 665. doi:10.1038/s41419-023-06203-2. https://pubmed.ncbi.nlm.nih.gov/37816734/
5. Huang, Yongkang, Qian, Yajuan, Xing, Yufei, Zhou, Tong, Shi, Minhua. 2023. POLRMT over-expression is linked to WNT/beta-catenin signaling, immune infiltration, and unfavorable outcomes in lung adenocarcinoma patients. In Cancer medicine, 12, 15691-15703. doi:10.1002/cam4.6174. https://pubmed.ncbi.nlm.nih.gov/37283308/
6. Kong, Yang, Li, Xiangrong, Zhang, Huanle, Yang, Hui-Lin, Dai, Jin. 2024. Targeting POLRMT by a first-in-class inhibitor IMT1 inhibits osteosarcoma cell growth in vitro and in vivo. In Cell death & disease, 15, 57. doi:10.1038/s41419-024-06444-9. https://pubmed.ncbi.nlm.nih.gov/38228583/
7. Inatomi, Teppei, Matsuda, Shigeru, Ishiuchi, Takashi, Yasukawa, Takehiro, Kang, Dongchon. 2021. TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication. In Biochimica et biophysica acta. Molecular cell research, 1869, 119167. doi:10.1016/j.bbamcr.2021.119167. https://pubmed.ncbi.nlm.nih.gov/34744028/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen