C57BL/6JCya-Fbxo25em1flox/Cya
Common Name
Fbxo25-flox
Product ID
S-CKO-18401
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-66822-Fbxo25-B6J-VB
Status
When using this mouse strain in a publication, please cite “Fbxo25-flox Mouse (Catalog S-CKO-18401) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Fbxo25-flox
Strain ID
CKOCMP-66822-Fbxo25-B6J-VB
Gene Name
Product ID
S-CKO-18401
Gene Alias
Fbx25, 9130015I06Rik
Background
C57BL/6JCya
Gene Full Name
F-box protein 25
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 8 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000209913
NCBI Transcript ID
NM_001347316
Target Region
Exon 4~5
Size of Effective Region
~2.9 kb
Overview of Gene Research
Fbxo25, an F-box protein, is a component of the SCF protein E3 ubiquitin ligase complex. It binds to substrates specifically, regulating multiple biological processes and pathways. For example, it is involved in MAPK signaling pathway regulation [2]. It also has significance in ubiquitination-related protein quality control, cellular signalings, and cell differentiation, especially in cardiac tissue [3]. Genetic models like gene knockout (KO) mouse models can be valuable for studying its functions.
In cutaneous squamous cell carcinoma (cSCC), stable silencing of Fbxo25 in SCC13 cells led to reduced tumor growth, with down-regulation of cyclin D1, indicating Fbxo25 promotes cSCC growth and metastasis through cyclin D1 [1]. In NSCLC, interference of Fbxo25 could significantly inhibit cell proliferation, invasion, and migration in H1299 cells, suggesting its role in promoting the tumorigenicity of lung cancer cells [4]. In cardiomyocyte development, silencing endogenous Fbxo25 increased Tbx5 and Nkx2-5 mRNA levels and suppressed mESC-derived cardiomyocyte differentiation, while exogenous expression of FBXO25 down-regulated NKX2-5 level in human ESC-derived cardiomyocytes [3]. In B cell lymphoma, monoallelic loss of Fbxo25 and stabilizing HAX1 phosphodegron mutations were identified, and knockdown of Fbxo25 significantly accelerated lymphoma development in Eμ-Myc mice and in a human MCL xenotransplant model, suggesting Fbxo25 functions as a haploinsufficient tumor suppressor [5].
In conclusion, Fbxo25 has diverse functions, regulating cell growth, metastasis in cancer, and cardiomyocyte development. KO/CKO mouse models have contributed to understanding its roles in diseases such as cSCC, NSCLC, and lymphoma, providing insights into potential therapeutic targets and disease mechanisms.
References：
1. Kuzmanov, Aleksandar, Johansen, Pål, Hofbauer, Günther. 2020. FBXO25 Promotes Cutaneous Squamous Cell Carcinoma Growth and Metastasis through Cyclin D1. In The Journal of investigative dermatology, 140, 2496-2504. doi:10.1016/j.jid.2020.04.003. https://pubmed.ncbi.nlm.nih.gov/32335130/
2. Teixeira, Felipe R, Manfiolli, Adriana O, Vieira, Nichelle A, Schechtman, Deborah, Gomes, Marcelo D. 2017. FBXO25 regulates MAPK signaling pathway through inhibition of ERK1/2 phosphorylation. In Archives of biochemistry and biophysics, 621, 38-45. doi:10.1016/j.abb.2017.04.003. https://pubmed.ncbi.nlm.nih.gov/28389297/
3. Jeong, Hoe-Su, Jung, Eun-Shil, Sim, Ye-Ji, Kim, Chang-Hoon, Kim, Kye-Seong. 2015. Fbxo25 controls Tbx5 and Nkx2-5 transcriptional activity to regulate cardiomyocyte development. In Biochimica et biophysica acta, 1849, 709-21. doi:10.1016/j.bbagrm.2015.02.002. https://pubmed.ncbi.nlm.nih.gov/25725482/
4. Jiang, Gui-Yang, Zhang, Xiu-Peng, Wang, Liang, Wang, En-Hua, Zhang, Yong. 2016. FBXO25 promotes cell proliferation, invasion, and migration of NSCLC. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37, 14311-14319. doi:. https://pubmed.ncbi.nlm.nih.gov/27596142/
5. Baumann, Ursula, Fernández-Sáiz, Vanesa, Rudelius, Martina, Kuster, Bernhard, Bassermann, Florian. 2014. Disruption of the PRKCD-FBXO25-HAX-1 axis attenuates the apoptotic response and drives lymphomagenesis. In Nature medicine, 20, 1401-9. doi:10.1038/nm.3740. https://pubmed.ncbi.nlm.nih.gov/25419709/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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