C57BL/6JCya-Chrna2em1flox/Cya
Common Name
Chrna2-flox
Product ID
S-CKO-18404
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-110902-Chrna2-B6J-VB
When using this mouse strain in a publication, please cite “Chrna2-flox Mouse (Catalog S-CKO-18404) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Chrna2-flox
Strain ID
CKOCMP-110902-Chrna2-B6J-VB
Gene Name
Product ID
S-CKO-18404
Gene Alias
Acra-2, Acra2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000022620
NCBI RefSeq
NM_144803
Target Region
Exon 3~4
Size of Effective Region
~1.6 kb
Overview of Gene Research
Chrna2, cholinergic receptor nicotinic alpha 2 subunit, is involved in multiple biological processes. In adipose tissue, it participates in a non-neuronal cholinergic signaling pathway that mediates beige thermoregulation, which is crucial for maintaining systemic metabolic homeostasis. It also has implications in neural circuits, with its expression in specific interneurons in the hippocampus and cortex influencing neural activities [1,3,4].
In fat-specific Chrna2 KO mice, thermogenic defects in cold and metabolic dysfunction upon dietary challenges were observed, indicating adipocyte-autonomous regulation in vivo. These findings suggest that the CHRNA2 pathway has biological significance in beige fat biogenesis and energy homeostasis [2].
In conclusion, Chrna2 is essential for beige thermoregulation and energy homeostasis as revealed through KO mouse model-based research. Its role in adipose tissue and neural circuits provides insights into metabolic diseases and neurological functions, highlighting its potential as a therapeutic target for combating metabolic diseases [1,2].
References：
1. Ma, Yingxu, Liu, Shanshan, Jun, Heejin, Wu, Jun. 2021. CHRNA2: a new paradigm in beige thermoregulation and metabolism. In Trends in cell biology, 32, 479-489. doi:10.1016/j.tcb.2021.11.009. https://pubmed.ncbi.nlm.nih.gov/34952750/
2. Jun, Heejin, Ma, Yingxu, Chen, Yong, Kajimura, Shingo, Wu, Jun. 2020. Adrenergic-Independent Signaling via CHRNA2 Regulates Beige Fat Activation. In Developmental cell, 54, 106-116.e5. doi:10.1016/j.devcel.2020.05.017. https://pubmed.ncbi.nlm.nih.gov/32533922/
3. Hilscher, Markus M, Nogueira, Ingrid, Mikulovic, Sanja, Leão, Richardson N, Leão, Katarina E. 2019. Chrna2-OLM interneurons display different membrane properties and h-current magnitude depending on dorsoventral location. In Hippocampus, 29, 1224-1237. doi:10.1002/hipo.23134. https://pubmed.ncbi.nlm.nih.gov/31301163/
4. Hilscher, Markus M, Leão, Richardson N, Edwards, Steven J, Leão, Katarina E, Kullander, Klas. 2017. Chrna2-Martinotti Cells Synchronize Layer 5 Type A Pyramidal Cells via Rebound Excitation. In PLoS biology, 15, e2001392. doi:10.1371/journal.pbio.2001392. https://pubmed.ncbi.nlm.nih.gov/28182735/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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