C57BL/6JCya-Acanem1flox/Cya
Common Name
Acan-flox
Product ID
S-CKO-18430
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-11595-Acan-B6J-VB
When using this mouse strain in a publication, please cite “Acan-flox Mouse (Catalog S-CKO-18430) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Acan-flox
Strain ID
CKOCMP-11595-Acan-B6J-VB
Gene Name
Product ID
S-CKO-18430
Gene Alias
Agc, Agc1, CSPCP, Cspg1, b2b183Clo, cmd
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000032835
NCBI RefSeq
NM_007424
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Overview of Gene Research
Acan, which encodes aggrecan, is a gene of significant importance. Aggrecan is a major proteoglycan component of the extracellular matrix in the growth plate and articular cartilage. It provides the hydrated gel structure crucial for the load-bearing properties of joints and is key in cartilage and bone morphogenesis. It may also be associated with the growth hormone pathway, as suggested by some studies [4].
In adult mice, deletion of Hdac2 in parvalbumin-positive (PV+) GABAergic cells leads to reduced Acan expression in these cells. This reduction is associated with a decrease in spontaneous recovery of fear memories, enhanced bouton remodeling in PV+ cells, and reduced perineuronal net aggregation around PV+ cells in the prefrontal cortex and basolateral amygdala [1].
In addition, ACAN heterozygous mutations in humans are linked to short stature. Some patients may have early-onset osteoarthritis, brachydactyly, midfacial hypoplasia, and early growth cessation. Growth-promoting therapies like rhGH and/or GnRHa can improve the adult height of affected pediatric patients [2,3,4,5,6,7]. In horses, heterozygotes for ACAN dwarfism alleles have reduced stature [8].
In conclusion, Acan plays essential roles in cartilage and bone development, and its regulation in PV+ GABAergic cells can impact fear memory recovery. Mouse models with targeted manipulation of genes related to Acan expression, such as Hdac2, have provided insights into its role in fear-related behaviors. In the context of human and animal growth, Acan mutations are associated with short stature, highlighting its importance in growth-related biological processes.
References：
1. Lavertu-Jolin, Marisol, Chattopadhyaya, Bidisha, Chehrazi, Pegah, Pineyro, Graçiela, Di Cristo, Graziella. 2023. Acan downregulation in parvalbumin GABAergic cells reduces spontaneous recovery of fear memories. In Molecular psychiatry, 28, 2946-2963. doi:10.1038/s41380-023-02085-0. https://pubmed.ncbi.nlm.nih.gov/37131076/
2. Tang, Wei, Wu, Ke-Mi, Zhou, Qiong, Dong, Guan-Ping, Zou, Chao-Chun. . Genotype and phenotype in patients with ACAN gene variants: Three cases and literature review. In Molecular genetics & genomic medicine, 12, e2439. doi:10.1002/mgg3.2439. https://pubmed.ncbi.nlm.nih.gov/38613222/
3. Deng, Shuyun, Hou, Lele, Xia, Dan, Ouyang, Nengtai, Liang, Liyang. 2022. Description of the molecular and phenotypic spectrum in Chinese patients with aggrecan deficiency: Novel ACAN heterozygous variants in eight Chinese children and a review of the literature. In Frontiers in endocrinology, 13, 1015954. doi:10.3389/fendo.2022.1015954. https://pubmed.ncbi.nlm.nih.gov/36387899/
4. Wu, Huiping, Wang, Chaoban, Yu, Shiwen, He, Pingping, Shan, Xiaoou. 2023. Downregulation of ACAN is Associated with the Growth hormone pathway and Induces short stature. In Journal of clinical laboratory analysis, 37, e24830. doi:10.1002/jcla.24830. https://pubmed.ncbi.nlm.nih.gov/36597844/
5. Masunaga, Yohei, Ohkubo, Yumiko, Nishimura, Gen, Saitsu, Hirotomo, Ogata, Tsutomu. 2022. ACAN biallelic variants in a girl with severe idiopathic short stature. In Journal of human genetics, 67, 481-486. doi:10.1038/s10038-022-01030-3. https://pubmed.ncbi.nlm.nih.gov/35314765/
6. Liang, Hanting, Miao, Hui, Pan, Hui, Wang, Linjie, Zhu, Huijuan. . Growth-Promoting Therapies May Be Useful In Short Stature Patients With Nonspecific Skeletal Abnormalities Caused By Acan Heterozygous Mutations: Six Chinese Cases And Literature Review. In Endocrine practice : official journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists, 26, 1255-1268. doi:10.4158/EP-2019-0518. https://pubmed.ncbi.nlm.nih.gov/33471655/
7. Wu, Su, Wang, Chunli, Cao, Qing, Gu, Wei, Li, Xiaonan. 2022. The Spectrum of ACAN Gene Mutations in a Selected Chinese Cohort of Short Stature: Genotype-Phenotype Correlation. In Frontiers in genetics, 13, 891040. doi:10.3389/fgene.2022.891040. https://pubmed.ncbi.nlm.nih.gov/35620465/
8. Graves, K T, Eberth, J E, Bailey, E. 2020. Heterozygotes for ACAN dwarfism alleles in horses have reduced stature. In Animal genetics, 51, 420-422. doi:10.1111/age.12921. https://pubmed.ncbi.nlm.nih.gov/32065671/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.