C57BL/6JCya-Polbem1flox/Cya
Common Name:
Polb-flox
Product ID:
S-CKO-18527
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Polb-flox
Strain ID
CKOCMP-18970-Polb-B6J-VB
Gene Name
Product ID
S-CKO-18527
Gene Alias
A430088C08Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Polbem1flox/Cya mice (Catalog S-CKO-18527) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033938
NCBI RefSeq
NM_011130
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Polb, encoding DNA polymerase β, is a member of the DNA polymerase X family. It is mainly involved in eukaryotic DNA replication, DNA damage repair (notably base excision repair-BER), gene recombination, and cell cycle regulation [1]. POLB's role in maintaining genome stability through BER is crucial for normal cellular function.
In mouse models, the POLB-Y265C mutation, resulting in an aberrant immune repertoire, leads to lupus-like disease. Mice with this mutation develop high levels of antinuclear antibodies, severe glomerulonephritis, and have shorter immunoglobulin heavy-chain junctions with increased somatic hypermutation [2,3,4]. Additionally, in MMR-deficient acute lymphoblastic leukemia cells, POLB plays a critical role in survival and thiopurine resistance. Depletion of POLB or treatment with its inhibitor oleanolic acid causes synthetic lethality, increasing cellular AP sites, DNA strand breaks, and apoptosis [5].
In conclusion, Polb is essential for DNA repair processes, especially BER. Mouse models with POLB mutations have been instrumental in revealing its role in autoimmune diseases like lupus and in understanding its contribution to drug resistance mechanisms in acute lymphoblastic leukemia. These findings enhance our understanding of the biological functions of Polb and offer potential therapeutic targets for related diseases.
References：
1. Zhang, Geyang, Wang, Jiamei, Li, Yulong, Wang, Eryao, Lyu, Shijie. 2024. POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes. In Animals : an open access journal from MDPI, 14, . doi:10.3390/ani14091323. https://pubmed.ncbi.nlm.nih.gov/38731327/
2. Paluri, Sesha L, Burak, Matthew, Senejani, Alireza G, Stephen Lloyd, R, Sweasy, Joann B. 2021. DNA glycosylase deficiency leads to decreased severity of lupus in the Polb-Y265C mouse model. In DNA repair, 105, 103152. doi:10.1016/j.dnarep.2021.103152. https://pubmed.ncbi.nlm.nih.gov/34186496/
3. Rahim, Tania, Levinson, Madison A, Carufe, Kelly E W, Gades, Naomi, Sweasy, Joann B. 2022. The hematopoietic compartment is sufficient for lupus development resulting from the POLB-Y265C mutation. In PloS one, 17, e0267913. doi:10.1371/journal.pone.0267913. https://pubmed.ncbi.nlm.nih.gov/35486639/
4. Senejani, Alireza G, Liu, Yanfeng, Kidane, Dawit, Bothwell, Alfred L M, Sweasy, Joann B. 2014. Mutation of POLB causes lupus in mice. In Cell reports, 6, 1-8. doi:10.1016/j.celrep.2013.12.017. https://pubmed.ncbi.nlm.nih.gov/24388753/
5. Teng, Ji-Yuan, Yang, Ding-Peng, Tang, Chao, Duan, Cai-Wen, Zhou, Bin-Bing S. 2023. Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia. In Leukemia, 37, 1204-1215. doi:10.1038/s41375-023-01902-3. https://pubmed.ncbi.nlm.nih.gov/37095208/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen