C57BL/6JCya-Tmem150aem1flox/Cya
Common Name:
Tmem150a-flox
Product ID:
S-CKO-18591
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem150a-flox
Strain ID
CKOCMP-232086-Tmem150a-B6J-VB
Gene Name
Product ID
S-CKO-18591
Gene Alias
Tmem150
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmem150aem1flox/Cya mice (Catalog S-CKO-18591) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069695
NCBI RefSeq
NM_144916
Target Region
Exon 5~8
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Tmem150a, also known as TM6P1 and damage-regulated autophagy modulator 5, is a transmembrane protein. It plays a crucial role in regulating phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex. This function is related to Toll-like receptor 4 (TLR4) signaling pathways, and thus has implications for cellular homeostasis and immune-related processes [1,3,4]. Genetic models, such as gene knockout (KO) models, are valuable for studying its functions.
Knockdown of Tmem150a in TLR4-expressing epithelial cells led to increased levels of lipopolysaccharide (LPS)-induced cytokine secretion and transcript levels. In a lung epithelial cell line (H292), Tmem150a knockdown also increased cytokine levels in unstimulated conditions, indicating its importance in cellular homeostasis [1]. In glioblastoma multiforme (GBM), overexpression of Tmem150a was observed in cancerous tissues. Suppressing Tmem150a expression in vitro inhibited GBM cell proliferation, migration, and invasion, suggesting its potential as a biomarker for poor prognosis in GBM patients [2].
In conclusion, Tmem150a is essential for regulating PI(4,5)P2 production and maintaining cellular homeostasis. Studies using KO or knockdown models have revealed its roles in cytokine regulation in epithelial cells and in the progression of glioblastoma multiforme. These findings contribute to our understanding of immune-related processes and cancer biology, potentially providing new directions for disease treatment and biomarker discovery.
References:
1. Romanet, Jessica L, Cupo, Katherine L, Yoder, Jeffrey A. . Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines. In Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 42, 336-342. doi:10.1089/jir.2022.0063. https://pubmed.ncbi.nlm.nih.gov/35834652/
2. Fan, Si-Tong, Xu, Hao-Qiang, He, Yang, Yang, Wen-Qiong, Qin, Yong. 2023. Overexpression of TMEM150A in glioblastoma multiforme patients correlated with dismal prognoses and compromised immune statuses. In PloS one, 18, e0294144. doi:10.1371/journal.pone.0294144. https://pubmed.ncbi.nlm.nih.gov/38055673/
3. Batrouni, Alex G, Bag, Nirmalya, Phan, Henry T, Baird, Barbara A, Baskin, Jeremy M. 2021. A palmitoylation code controls PI4KIIIα complex formation and PI(4,5)P2 homeostasis at the plasma membrane. In Journal of cell science, 135, . doi:10.1242/jcs.259365. https://pubmed.ncbi.nlm.nih.gov/34569608/
4. Chung, Jeeyun, Nakatsu, Fubito, Baskin, Jeremy M, De Camilli, Pietro. 2015. Plasticity of PI4KIIIα interactions at the plasma membrane. In EMBO reports, 16, 312-20. doi:10.15252/embr.201439151. https://pubmed.ncbi.nlm.nih.gov/25608530/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen