C57BL/6JCya-Lin9em1flox/Cya
Common Name
Lin9-flox
Product ID
S-CKO-18595
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-72568-Lin9-B6J-VB
Status
When using this mouse strain in a publication, please cite “Lin9-flox Mouse (Catalog S-CKO-18595) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Lin9-flox
Strain ID
CKOCMP-72568-Lin9-B6J-VB
Gene Name
Product ID
S-CKO-18595
Gene Alias
TGS, Bara, TGS1, Lin-9, mLin-9, 2700022J23Rik
Background
C57BL/6JCya
Gene Full Name
lin-9 DREAM MuvB core complex component
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 1 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000192561
NCBI Transcript ID
NM_001103182
Target Region
Exon 7
Size of Effective Region
~0.7 kb
Overview of Gene Research
Lin9 is a subunit of the DREAM complex, playing a crucial role in regulating cell mitotic processes, cell-cycle progression, and gene expression during the cell cycle [3,4]. It is associated with pathways related to cell proliferation, and its proper function is of great biological importance for embryonic development, cell survival, and maintaining genome stability [3,4,5]. Genetic models, such as gene-knockout (KO) models in mice and zebrafish, have been valuable for studying Lin9 [4,5].
In KO mouse models, loss of Lin9 abolishes cell proliferation and leads to multiple mitotic and cytokinesis defects due to down-regulation of a large set of mitotic genes like Plk1, Aurora A, and Kif20a, indicating its essential role in early embryonic development and adult mouse viability. Lin9-heterozygous mice are more susceptible to lung tumorigenesis induced by oncogenic c-Raf, suggesting it may function as a haploinsufficient tumor suppressor [4]. In zebrafish, Lin9-depleted embryonic cells accumulate in mitosis, followed by apoptosis, especially in the developing central nervous system, and a cohort of Lin9-regulated genes for different mitotic processes was identified [5].
In conclusion, Lin9 is essential for regulating mitosis, cell proliferation, and genome stability. Studies using KO mouse and zebrafish models have revealed its significance in embryonic development and tumor suppression. High Lin9 expression is also related to poor clinical outcomes and drug resistance in some cancers, such as triple-negative breast cancer and lung adenocarcinoma, highlighting its potential as a therapeutic target in cancer treatment [1,2,6].
References：
1. Lai, Hongna, Wang, Rui, Li, Shunying, Li, Yudong, Liu, Yujie. 2019. LIN9 confers paclitaxel resistance in triple negative breast cancer cells by upregulating CCSAP. In Science China. Life sciences, 63, 419-428. doi:10.1007/s11427-019-9581-8. https://pubmed.ncbi.nlm.nih.gov/31420851/
2. Roberts, Melyssa S, Sahni, Jennifer M, Schrock, Morgan S, Summers, Matthew K, Keri, Ruth A. 2020. LIN9 and NEK2 Are Core Regulators of Mitotic Fidelity That Can Be Therapeutically Targeted to Overcome Taxane Resistance. In Cancer research, 80, 1693-1706. doi:10.1158/0008-5472.CAN-19-3466. https://pubmed.ncbi.nlm.nih.gov/32054769/
3. Esterlechner, Jasmina, Reichert, Nina, Iltzsche, Fabian, Finkernagel, Florian, Gaubatz, Stefan. 2013. LIN9, a subunit of the DREAM complex, regulates mitotic gene expression and proliferation of embryonic stem cells. In PloS one, 8, e62882. doi:10.1371/journal.pone.0062882. https://pubmed.ncbi.nlm.nih.gov/23667535/
4. Reichert, Nina, Wurster, Sebastian, Ulrich, Tanja, Rapp, Ulf, Gaubatz, Stefan. 2010. Lin9, a subunit of the mammalian DREAM complex, is essential for embryonic development, for survival of adult mice, and for tumor suppression. In Molecular and cellular biology, 30, 2896-908. doi:10.1128/MCB.00028-10. https://pubmed.ncbi.nlm.nih.gov/20404087/
5. Kleinschmidt, Markus A, Wagner, Toni U, Liedtke, Daniel, Samans, Birgit, Gaubatz, Stefan. 2009. lin9 is required for mitosis and cell survival during early zebrafish development. In The Journal of biological chemistry, 284, 13119-27. doi:10.1074/jbc.M809635200. https://pubmed.ncbi.nlm.nih.gov/19278998/
6. Hou, Qinghua, Zhong, Yanfeng, Liao, Mengying, Li, Xiaoqing, Liu, Jixian. 2024. Upregulation of the tumor suppressor gene LIN9 enhances tumorigenesis and predicts poor prognosis of lung adenocarcinoma. In Heliyon, 10, e35012. doi:10.1016/j.heliyon.2024.e35012. https://pubmed.ncbi.nlm.nih.gov/39157309/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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