C57BL/6JCya-Fbxo28em1flox/Cya
Common Name:
Fbxo28-flox
Product ID:
S-CKO-18600
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fbxo28-flox
Strain ID
CKOCMP-67948-Fbxo28-B6J-VB
Gene Name
Product ID
S-CKO-18600
Gene Alias
4833428J17Rik; 5730505P19Rik; D1Ertd578e; Fbx28; mKIAA0483
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbxo28em1flox/Cya mice (Catalog S-CKO-18600) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000051431
NCBI RefSeq
NM_175127
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Fbxo28, a member of F-box proteins, serves as the substrate receptor of SCF (SKP1, CULLIN1, F-box protein) ubiquitin ligase complexes. It is involved in numerous cellular processes through ubiquitination-mediated protein degradation, regulating pathways related to cell division, metabolism, and cancer-related processes [1,2,3,4,5,6,7]. Genetic models, such as mouse models, are valuable for studying its functions.
In mouse oocytes, depletion of Fbxo28 using morpholino oligonucleotides led to defects in spindle morphology and migration during meiosis I, disrupting cortical and cytoplasmic actin assembly, indicating its essential role in asymmetric oocyte division [2]. In hyperlipidemic mice, upregulation of Fbxo28 alleviated abnormal lipid metabolism and inflammatory responses, reducing high-fat diet-induced hyperlipidemia by promoting the ubiquitinated degradation of RAB27A [5].
In conclusion, Fbxo28 is crucial for various biological processes. Its functions in spindle migration during oocyte meiosis and lipid metabolism in hyperlipidemia are revealed through mouse models. Understanding Fbxo28 may provide insights into reproductive biology and the treatment of hyperlipidemia.
References：
1. Qiao, Xinran, Lin, Jingyu, Shen, Jiajia, Li, Pengyu, Wang, Zhen. 2023. FBXO28 suppresses liver cancer invasion and metastasis by promoting PKA-dependent SNAI2 degradation. In Oncogene, 42, 2878-2891. doi:10.1038/s41388-023-02809-0. https://pubmed.ncbi.nlm.nih.gov/37596321/
2. Chang, Haoya, Huang, Chenyang, Cheng, Siyu, Li, Jian, Wang, Xiaohong. 2024. Fbxo28 is essential for spindle migration and morphology during mouse oocyte meiosis I. In International journal of biological macromolecules, 275, 133232. doi:10.1016/j.ijbiomac.2024.133232. https://pubmed.ncbi.nlm.nih.gov/38960234/
3. Liu, Songbai, Liu, Peng, Zhu, Changhao, Wang, Xing, Pan, Yaozhen. 2023. FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination. In Aging, 15, 5381-5398. doi:10.18632/aging.204780. https://pubmed.ncbi.nlm.nih.gov/37348029/
4. Cai, Lili, Liu, Liang, Li, Lihui, Jia, Lijun. 2019. SCFFBXO28-mediated self-ubiquitination of FBXO28 promotes its degradation. In Cellular signalling, 65, 109440. doi:10.1016/j.cellsig.2019.109440. https://pubmed.ncbi.nlm.nih.gov/31678254/
5. Sun, J, Du, B, Chen, M, Wang, X, Hong, J. 2024. FBXO28 reduces high-fat diet-induced hyperlipidemia in mice by alleviating abnormal lipid metabolism and inflammatory responses. In Journal of endocrinological investigation, 47, 2757-2774. doi:10.1007/s40618-024-02376-5. https://pubmed.ncbi.nlm.nih.gov/38696123/
6. Song, Gendi, Sun, Zhengwei, Chu, Man, Wang, Zhiwei, Zhu, Xueqiong. 2024. FBXO28 promotes cell proliferation, migration and invasion via upregulation of the TGF-beta1/SMAD2/3 signaling pathway in ovarian cancer. In BMC cancer, 24, 122. doi:10.1186/s12885-024-11893-8. https://pubmed.ncbi.nlm.nih.gov/38267923/
7. Phillips, Emma, Balss, Jörg, Bethke, Frederic, Fendt, Sarah-Maria, Goidts, Violaine. 2022. PFKFB4 interacts with FBXO28 to promote HIF-1α signaling in glioblastoma. In Oncogenesis, 11, 57. doi:10.1038/s41389-022-00433-3. https://pubmed.ncbi.nlm.nih.gov/36115843/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen